Matches in SemOpenAlex for { <https://semopenalex.org/work/W4322488467> ?p ?o ?g. }
- W4322488467 abstract "Hepatitis E virus (HEV) is an RNA virus responsible for over 20 million infections annually. HEV’s open reading frame (ORF)1 polyprotein is essential for genome replication, though it is unknown how the different subdomains function within a structural context. Our data show that ORF1 operates as a multifunctional protein, which is not subject to proteolytic processing. Supporting this model, scanning mutagenesis performed on the putative papain-like cysteine protease (pPCP) domain revealed six cysteines essential for viral replication. Our data are consistent with their role in divalent metal ion coordination, which governs local and interdomain interactions that are critical for the overall structure of ORF1; furthermore, the ‘pPCP’ domain can only rescue viral genome replication in trans when expressed in the context of the full-length ORF1 protein but not as an individual subdomain. Taken together, our work provides a comprehensive model of the structure and function of HEV ORF1." @default.
- W4322488467 created "2023-02-28" @default.
- W4322488467 creator A5019050816 @default.
- W4322488467 creator A5029895540 @default.
- W4322488467 creator A5036004411 @default.
- W4322488467 creator A5049329527 @default.
- W4322488467 creator A5058080042 @default.
- W4322488467 creator A5087997570 @default.
- W4322488467 creator A5091225024 @default.
- W4322488467 date "2023-02-28" @default.
- W4322488467 modified "2023-10-16" @default.
- W4322488467 title "Structural features stabilized by divalent cation coordination within hepatitis E virus ORF1 are critical for viral replication" @default.
- W4322488467 cites W1551265504 @default.
- W4322488467 cites W168725157 @default.
- W4322488467 cites W1963538134 @default.
- W4322488467 cites W1964519906 @default.
- W4322488467 cites W1971703154 @default.
- W4322488467 cites W1977655940 @default.
- W4322488467 cites W1987962702 @default.
- W4322488467 cites W2003144438 @default.
- W4322488467 cites W2003719005 @default.
- W4322488467 cites W2008294207 @default.
- W4322488467 cites W2012808888 @default.
- W4322488467 cites W2029274089 @default.
- W4322488467 cites W2030376227 @default.
- W4322488467 cites W2032567331 @default.
- W4322488467 cites W2033125798 @default.
- W4322488467 cites W2039620306 @default.
- W4322488467 cites W2045058866 @default.
- W4322488467 cites W2047910097 @default.
- W4322488467 cites W2063348935 @default.
- W4322488467 cites W2065263745 @default.
- W4322488467 cites W2067431728 @default.
- W4322488467 cites W2067488895 @default.
- W4322488467 cites W2069835701 @default.
- W4322488467 cites W2070633240 @default.
- W4322488467 cites W2075659454 @default.
- W4322488467 cites W2079145977 @default.
- W4322488467 cites W2082439356 @default.
- W4322488467 cites W2084208175 @default.
- W4322488467 cites W2086004174 @default.
- W4322488467 cites W2098587625 @default.
- W4322488467 cites W2102245393 @default.
- W4322488467 cites W2111627216 @default.
- W4322488467 cites W2121313939 @default.
- W4322488467 cites W2122994445 @default.
- W4322488467 cites W2125079066 @default.
- W4322488467 cites W2132629607 @default.
- W4322488467 cites W2133706960 @default.
- W4322488467 cites W2138892620 @default.
- W4322488467 cites W2139079446 @default.
- W4322488467 cites W2148853951 @default.
- W4322488467 cites W2152178770 @default.
- W4322488467 cites W2154627118 @default.
- W4322488467 cites W2156661615 @default.
- W4322488467 cites W2158290219 @default.
- W4322488467 cites W2168479171 @default.
- W4322488467 cites W2528181301 @default.
- W4322488467 cites W2573091838 @default.
- W4322488467 cites W2578489854 @default.
- W4322488467 cites W2606381431 @default.
- W4322488467 cites W2784305745 @default.
- W4322488467 cites W2800061958 @default.
- W4322488467 cites W2807766837 @default.
- W4322488467 cites W2809050289 @default.
- W4322488467 cites W2898674143 @default.
- W4322488467 cites W2950873526 @default.
- W4322488467 cites W2951367475 @default.
- W4322488467 cites W2962634872 @default.
- W4322488467 cites W2967681833 @default.
- W4322488467 cites W3001821095 @default.
- W4322488467 cites W3112039569 @default.
- W4322488467 cites W3177828909 @default.
- W4322488467 cites W3183475563 @default.
- W4322488467 cites W3190214202 @default.
- W4322488467 cites W4213149192 @default.
- W4322488467 cites W4213445308 @default.
- W4322488467 doi "https://doi.org/10.7554/elife.80529" @default.
- W4322488467 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36852909" @default.
- W4322488467 hasPublicationYear "2023" @default.
- W4322488467 type Work @default.
- W4322488467 citedByCount "4" @default.
- W4322488467 countsByYear W43224884672023 @default.
- W4322488467 crossrefType "journal-article" @default.
- W4322488467 hasAuthorship W4322488467A5019050816 @default.
- W4322488467 hasAuthorship W4322488467A5029895540 @default.
- W4322488467 hasAuthorship W4322488467A5036004411 @default.
- W4322488467 hasAuthorship W4322488467A5049329527 @default.
- W4322488467 hasAuthorship W4322488467A5058080042 @default.
- W4322488467 hasAuthorship W4322488467A5087997570 @default.
- W4322488467 hasAuthorship W4322488467A5091225024 @default.
- W4322488467 hasBestOaLocation W43224884671 @default.
- W4322488467 hasConcept C104317684 @default.
- W4322488467 hasConcept C135763542 @default.
- W4322488467 hasConcept C140704245 @default.
- W4322488467 hasConcept C151730666 @default.
- W4322488467 hasConcept C159047783 @default.
- W4322488467 hasConcept C167625842 @default.
- W4322488467 hasConcept C185592680 @default.
- W4322488467 hasConcept C2522874641 @default.