Matches in SemOpenAlex for { <https://semopenalex.org/work/W4322503788> ?p ?o ?g. }
- W4322503788 endingPage "226" @default.
- W4322503788 startingPage "226" @default.
- W4322503788 abstract "Strain ST182Gu, isolated from fresh guava fruit, was identified as Enterococcus casseliflavus on the basis of biochemical tests, sugar fermentation reactions (API20Strip), PCR with genus-specific primers, and 16S rRNA sequencing. This appears to be the first documentation of the presence of this species in guava. E. casseliflavus ST182Gu was shown to produce a 4.8 kDa class IIa bacteriocin, active against various lactic acid bacteria including Enterococcus spp. and Streptococcus spp., and Staphylococcus aureus, and different serotypes of Listeria spp. The activity of the peptide was reduced by treatment with 0.1 mg/mL proteolytic enzymes, but not by α-amylase, catalase, lipase, and 1% (w/v) sodium dodecyl sulphate (SDS), Tween-20, Tween-80, urea, NaCl, and EDTA. No change in activity was recorded after adjustment to pH values of between 2.0 and 12.0 for 2 h, and after treatment at 100 °C for 120 min or 121°C for 20 min, compared with non-treated antimicrobial peptide. The mode of action against representative susceptible bacteria was shown to be bactericidal and associated with cell lysis and enzyme- and DNA-leakage. These susceptible bacteria, Listeria ivanovii subsp. ivanovii ATCC 19119, Listeria monocytogenes ATCC 15313, and Enterococcus faecalis ATCC 19443 differed however in their sensitivity to bacteriocin ST182Gu (6,553,600 AU/mL, 102,400 AU/mL, and 51,200 AU/mL, respectively). No significant differences were detected in cell growth and bacteriocin production when strain ST182Gu was grown in MRS broth at 26 °C, 30 °C, and 37 °C for 24 h. Bacteriocin ST182Gu recovery from the surface of the producer cells showed different activity, dependent of the applied test organisms (3200, 800 and 400 AU/mL, evaluated versus L. ivanovii subsp. ivanovii ATCC 19119, L. monocytogenes ATCC 15313 and E. faecalis ATCC 19443, respectively), however, with proportional values with the activity recorded in cell free supernatant versus same test microorganisms. When bacteriocin ST182Gu was combined with sublethal doses of ciprofloxacin, synergistic inhibition of L. ivanovii subsp. ivanovii ATCC 19119 was demonstrated. This increase in ciprofloxacin sensitivity may be due to the dissipation of the proton gradient in the cell membrane of the target organism associated with exposure to bacteriocin ST182Gu. Apart from reducing the MIC of classical therapeutic antibiotics, bacteriocins such as ST182Gu may also play an important role in the treatment of multidrug resistant strains." @default.
- W4322503788 created "2023-02-28" @default.
- W4322503788 creator A5023355690 @default.
- W4322503788 creator A5032975513 @default.
- W4322503788 creator A5086931633 @default.
- W4322503788 date "2023-02-27" @default.
- W4322503788 modified "2023-10-14" @default.
- W4322503788 title "Bacteriocinogenic Enterococcus casseliflavus Isolated from Fresh Guava Fruit (Psidium guajava): Characterization of Bacteriocin ST192Gu and Some Aspects of Its Mode of Action on Listeria spp. and Enterococcus spp." @default.
- W4322503788 cites W1518315904 @default.
- W4322503788 cites W1900333136 @default.
- W4322503788 cites W1966420655 @default.
- W4322503788 cites W1983421127 @default.
- W4322503788 cites W1988194112 @default.
- W4322503788 cites W1994653485 @default.
- W4322503788 cites W2002679554 @default.
- W4322503788 cites W2003093218 @default.
- W4322503788 cites W2005119687 @default.
- W4322503788 cites W2011437992 @default.
- W4322503788 cites W2014587515 @default.
- W4322503788 cites W2018530742 @default.
- W4322503788 cites W2024957020 @default.
- W4322503788 cites W2030943800 @default.
- W4322503788 cites W2038443193 @default.
- W4322503788 cites W2039908600 @default.
- W4322503788 cites W2040538609 @default.
- W4322503788 cites W2053872745 @default.
- W4322503788 cites W2062716714 @default.
- W4322503788 cites W2066360431 @default.
- W4322503788 cites W2072005570 @default.
- W4322503788 cites W2084785831 @default.
- W4322503788 cites W2098586412 @default.
- W4322503788 cites W2112795866 @default.
- W4322503788 cites W2114274249 @default.
- W4322503788 cites W2118910851 @default.
- W4322503788 cites W2128965279 @default.
- W4322503788 cites W2130332666 @default.
- W4322503788 cites W2144633410 @default.
- W4322503788 cites W2151627786 @default.
- W4322503788 cites W2160918628 @default.
- W4322503788 cites W2161322062 @default.
- W4322503788 cites W2176673138 @default.
- W4322503788 cites W2189603610 @default.
- W4322503788 cites W2233236537 @default.
- W4322503788 cites W2743823731 @default.
- W4322503788 cites W2916766366 @default.
- W4322503788 cites W3000476497 @default.
- W4322503788 cites W3003091937 @default.
- W4322503788 cites W3095760790 @default.
- W4322503788 cites W3104614903 @default.
- W4322503788 cites W3161206131 @default.
- W4322503788 cites W3181372096 @default.
- W4322503788 cites W3200485663 @default.
- W4322503788 cites W4206912081 @default.
- W4322503788 cites W4220856982 @default.
- W4322503788 cites W4244089954 @default.
- W4322503788 cites W4290642912 @default.
- W4322503788 cites W95054765 @default.
- W4322503788 doi "https://doi.org/10.3390/fermentation9030226" @default.
- W4322503788 hasPublicationYear "2023" @default.
- W4322503788 type Work @default.
- W4322503788 citedByCount "1" @default.
- W4322503788 countsByYear W43225037882023 @default.
- W4322503788 crossrefType "journal-article" @default.
- W4322503788 hasAuthorship W4322503788A5023355690 @default.
- W4322503788 hasAuthorship W4322503788A5032975513 @default.
- W4322503788 hasAuthorship W4322503788A5086931633 @default.
- W4322503788 hasBestOaLocation W43225037881 @default.
- W4322503788 hasConcept C115574571 @default.
- W4322503788 hasConcept C185592680 @default.
- W4322503788 hasConcept C2777677444 @default.
- W4322503788 hasConcept C2778682378 @default.
- W4322503788 hasConcept C2779489039 @default.
- W4322503788 hasConcept C2779895986 @default.
- W4322503788 hasConcept C2780585763 @default.
- W4322503788 hasConcept C2781350384 @default.
- W4322503788 hasConcept C4937899 @default.
- W4322503788 hasConcept C501593827 @default.
- W4322503788 hasConcept C523546767 @default.
- W4322503788 hasConcept C54355233 @default.
- W4322503788 hasConcept C86803240 @default.
- W4322503788 hasConcept C89423630 @default.
- W4322503788 hasConceptScore W4322503788C115574571 @default.
- W4322503788 hasConceptScore W4322503788C185592680 @default.
- W4322503788 hasConceptScore W4322503788C2777677444 @default.
- W4322503788 hasConceptScore W4322503788C2778682378 @default.
- W4322503788 hasConceptScore W4322503788C2779489039 @default.
- W4322503788 hasConceptScore W4322503788C2779895986 @default.
- W4322503788 hasConceptScore W4322503788C2780585763 @default.
- W4322503788 hasConceptScore W4322503788C2781350384 @default.
- W4322503788 hasConceptScore W4322503788C4937899 @default.
- W4322503788 hasConceptScore W4322503788C501593827 @default.
- W4322503788 hasConceptScore W4322503788C523546767 @default.
- W4322503788 hasConceptScore W4322503788C54355233 @default.
- W4322503788 hasConceptScore W4322503788C86803240 @default.
- W4322503788 hasConceptScore W4322503788C89423630 @default.
- W4322503788 hasFunder F4320323339 @default.
- W4322503788 hasIssue "3" @default.
- W4322503788 hasLocation W43225037881 @default.