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- W4322707227 abstract "High molecular weight causes chitosan to have low solubility and high viscosity, limiting its use in the food and medical fields, especially at pH above seven. Therefore, increasing solubility and decreasing viscosity are important steps in chitosan application. One way to reduce the molecular weight of chitosan is through the enzymatic hydrolysis process. Microwave is an electromagnetic wave with frequencies of 300 MHz to 300,000 MHz. It is generally used for telecommunications, radar, and heating sources for both domestic cooking and industrial processes. Microwaves have been shown to increase the yield and selectivity of chemical reactions by reducing reaction times. The effect of microwave irradiation as a pretreatment process to increase the reaction rate and its hydrolysis products characteristics would be observed in this research. After pretreatment, enzymatic hydrolysis of chitosan was carried out using cellulase enzymes and added by surfactant span 80. The total reducing sugars of hydrolysis products were then measured as well as the viscosity and molecular weight. FTIR analysis was conducted to determine the functional groups within the hydrolysis products. Microwave irradiation greatly increased the hydrolysis reaction rate, shown by the increasing total reducing sugars. Higher microwave power usage and longer irradiation time also increased the total reducing sugars. The optimum condition of the microwave irradiation process found was at 270 watts for 7 minutes. The microwave pretreatment and the surfactant addition could reduce the viscosity and molecular weight of hydrolized chitosan product. It also did not chemically change the structure of the hydrolysis products." @default.
- W4322707227 created "2023-03-02" @default.
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- W4322707227 date "2023-01-01" @default.
- W4322707227 modified "2023-09-25" @default.
- W4322707227 title "Microwave-assisted pretreatment and span 80 addition to improve the enzymatic hydrolysis of chitosan" @default.
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- W4322707227 doi "https://doi.org/10.1063/5.0129783" @default.
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