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- W4322731639 abstract "We propose an oil-sealed arginine-glycine-aspartate (RGD)-modified hydrogel microwell array for analyzing exosomes secreted from single cells. Our device realizes the collection of exosomes from single cells in parallel. The size-selective permeation of the RGD-modified alginate hydrogel allows both stable cell culturing of single or few cells in closed wells and the confinement of exosomes secreted in each space. The nutrients (< 20 nm) that are necessary for cell culture can pass through the hydrogel, while exosomes (30-150 nm) do not permeate the walls of microwells. The cell culture property and the permeability of our device were examined, showing the capability to collect exosomes from single cells. We believe that our device would contribute to understanding the mechanisms of various diseases." @default.
- W4322731639 created "2023-03-03" @default.
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- W4322731639 date "2023-01-15" @default.
- W4322731639 modified "2023-09-24" @default.
- W4322731639 title "Oil-Sealed Rgd-Modified Hydrogel Microwell Array with Size-Selective Permeation for Analysis on Exosomes from Single Cells" @default.
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- W4322731639 doi "https://doi.org/10.1109/mems49605.2023.10052318" @default.
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