Matches in SemOpenAlex for { <https://semopenalex.org/work/W4322743952> ?p ?o ?g. }
- W4322743952 endingPage "2271" @default.
- W4322743952 startingPage "2271" @default.
- W4322743952 abstract "Glypican-3 (GPC3), as an emerging biomarker, has been shown to be beneficial for the early diagnosis and treatment of hepatocellular carcinoma (HCC). In this study, an ultrasensitive electrochemical biosensor for GPC3 detection has been constructed based on the hemin-reduced graphene oxide-palladium nanoparticles (H-rGO-Pd NPs) nanozyme-enhanced silver deposition signal amplification strategy. When GPC3 specifically interacted with GPC3 antibody (GPC3Ab) and GPC3 aptamer (GPC3Apt), an H-rGO-Pd NPs-GPC3Apt/GPC3/GPC3Ab sandwich complex was formed with peroxidase-like properties which enhanced H2O2 to reduce the silver (Ag) ions in solution to metallic Ag, resulting in the deposition of silver nanoparticles (Ag NPs) on the surface of the biosensor. The amount of deposited Ag, which was derived from the amount of GPC3, was quantified by the differential pulse voltammetry (DPV) method. Under ideal circumstances, the response value was linearly correlated with GPC3 concentration at 10.0-100.0 μg/mL with R2 of 0.9715. When the GPC3 concentration was in the range from 0.01 to 10.0 μg/mL, the response value was logarithmically linear with the GPC3 concentration with R2 of 0.9941. The limit of detection was 3.30 ng/mL at a signal-to-noise ratio of three and the sensitivity was 1.535 μAμM-1cm-2. Furthermore, the electrochemical biosensor detected the GPC3 level in actual serum samples with good recoveries (103.78-106.52%) and satisfactory relative standard deviations (RSDs) (1.89-8.81%), which confirmed the applicability of the sensor in practical applications. This study provides a new analytical method for measuring the level of GPC3 in the early diagnosis of HCC." @default.
- W4322743952 created "2023-03-03" @default.
- W4322743952 creator A5002322175 @default.
- W4322743952 creator A5006069034 @default.
- W4322743952 creator A5029556258 @default.
- W4322743952 creator A5030442700 @default.
- W4322743952 creator A5043513224 @default.
- W4322743952 creator A5056168495 @default.
- W4322743952 creator A5072861069 @default.
- W4322743952 creator A5087893740 @default.
- W4322743952 creator A5089781808 @default.
- W4322743952 date "2023-02-28" @default.
- W4322743952 modified "2023-10-04" @default.
- W4322743952 title "H-rGO-Pd NPs Nanozyme Enhanced Silver Deposition Strategy for Electrochemical Detection of Glypican-3" @default.
- W4322743952 cites W1539688055 @default.
- W4322743952 cites W2023822209 @default.
- W4322743952 cites W2607466521 @default.
- W4322743952 cites W2752721286 @default.
- W4322743952 cites W2795122808 @default.
- W4322743952 cites W2795772359 @default.
- W4322743952 cites W2883291178 @default.
- W4322743952 cites W2918405227 @default.
- W4322743952 cites W2946491385 @default.
- W4322743952 cites W2950212523 @default.
- W4322743952 cites W2958814732 @default.
- W4322743952 cites W2962350738 @default.
- W4322743952 cites W2969057759 @default.
- W4322743952 cites W2969802526 @default.
- W4322743952 cites W3004580407 @default.
- W4322743952 cites W3013203098 @default.
- W4322743952 cites W3013216743 @default.
- W4322743952 cites W3021664142 @default.
- W4322743952 cites W3033757821 @default.
- W4322743952 cites W3046724981 @default.
- W4322743952 cites W3102633766 @default.
- W4322743952 cites W3123769607 @default.
- W4322743952 cites W3135831599 @default.
- W4322743952 cites W3142234919 @default.
- W4322743952 cites W3157188545 @default.
- W4322743952 cites W3163548991 @default.
- W4322743952 cites W3180890399 @default.
- W4322743952 cites W3202886577 @default.
- W4322743952 cites W3207578163 @default.
- W4322743952 cites W3211514363 @default.
- W4322743952 cites W4200254003 @default.
- W4322743952 cites W4200520158 @default.
- W4322743952 cites W4205125932 @default.
- W4322743952 cites W4205720310 @default.
- W4322743952 cites W4210385348 @default.
- W4322743952 cites W4220674279 @default.
- W4322743952 cites W4225265470 @default.
- W4322743952 cites W4229061918 @default.
- W4322743952 cites W4281871223 @default.
- W4322743952 cites W4283071493 @default.
- W4322743952 cites W4283709532 @default.
- W4322743952 cites W4284989416 @default.
- W4322743952 cites W4297201621 @default.
- W4322743952 doi "https://doi.org/10.3390/molecules28052271" @default.
- W4322743952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36903516" @default.
- W4322743952 hasPublicationYear "2023" @default.
- W4322743952 type Work @default.
- W4322743952 citedByCount "3" @default.
- W4322743952 countsByYear W43227439522023 @default.
- W4322743952 crossrefType "journal-article" @default.
- W4322743952 hasAuthorship W4322743952A5002322175 @default.
- W4322743952 hasAuthorship W4322743952A5006069034 @default.
- W4322743952 hasAuthorship W4322743952A5029556258 @default.
- W4322743952 hasAuthorship W4322743952A5030442700 @default.
- W4322743952 hasAuthorship W4322743952A5043513224 @default.
- W4322743952 hasAuthorship W4322743952A5056168495 @default.
- W4322743952 hasAuthorship W4322743952A5072861069 @default.
- W4322743952 hasAuthorship W4322743952A5087893740 @default.
- W4322743952 hasAuthorship W4322743952A5089781808 @default.
- W4322743952 hasBestOaLocation W43227439521 @default.
- W4322743952 hasConcept C10349863 @default.
- W4322743952 hasConcept C119128265 @default.
- W4322743952 hasConcept C128972844 @default.
- W4322743952 hasConcept C13965031 @default.
- W4322743952 hasConcept C147789679 @default.
- W4322743952 hasConcept C155672457 @default.
- W4322743952 hasConcept C160756335 @default.
- W4322743952 hasConcept C171250308 @default.
- W4322743952 hasConcept C17525397 @default.
- W4322743952 hasConcept C185592680 @default.
- W4322743952 hasConcept C192562407 @default.
- W4322743952 hasConcept C2777869211 @default.
- W4322743952 hasConcept C2778019345 @default.
- W4322743952 hasConcept C2779525745 @default.
- W4322743952 hasConcept C2779742787 @default.
- W4322743952 hasConcept C30080830 @default.
- W4322743952 hasConcept C31499863 @default.
- W4322743952 hasConcept C43617362 @default.
- W4322743952 hasConcept C502942594 @default.
- W4322743952 hasConcept C52859227 @default.
- W4322743952 hasConcept C54355233 @default.
- W4322743952 hasConcept C86803240 @default.
- W4322743952 hasConceptScore W4322743952C10349863 @default.
- W4322743952 hasConceptScore W4322743952C119128265 @default.