Matches in SemOpenAlex for { <https://semopenalex.org/work/W4322753699> ?p ?o ?g. }
- W4322753699 endingPage "2176" @default.
- W4322753699 startingPage "2169" @default.
- W4322753699 abstract "Opsins are photosensitive G protein-coupled receptor proteins and are classified into visual and nonvisual receptors. Opn5L1 is a nonvisual opsin that binds all-trans retinal as a chromophore. A unique feature of Opn5L1 is that the protein exhibits a photocyclic reaction upon photoexcitation. Determining the chromophore structures of intermediates in the photocycle is essential for understanding the functional mechanism of Opn5L1. A previous study revealed that a long-lived intermediate in the photocycle cannot activate the G protein and forms a covalent bond between the retinal chromophore and a nearby cysteine residue. However, the position of this covalent bond in the chromophore remains undetermined. Here, we report a resonance Raman study on isotopically labeled samples in combination with density functional theory calculations and reveal that the 11th carbon atom of the chromophore of the intermediate forms a covalent linkage to the cysteine residue. Furthermore, vibrational assignments based on the isotopic substitutions and density functional theory calculations suggested that the Schiff base of the intermediate is deprotonated. The chromophore structure determined in the present study well explains the mechanism of the photocyclic reaction, which is crucial to the photobiological function of Opn5L1." @default.
- W4322753699 created "2023-03-03" @default.
- W4322753699 creator A5001842284 @default.
- W4322753699 creator A5008838993 @default.
- W4322753699 creator A5012095999 @default.
- W4322753699 creator A5019857622 @default.
- W4322753699 creator A5026074190 @default.
- W4322753699 creator A5033376423 @default.
- W4322753699 creator A5048875279 @default.
- W4322753699 creator A5065043759 @default.
- W4322753699 creator A5084042493 @default.
- W4322753699 creator A5086925320 @default.
- W4322753699 date "2023-03-01" @default.
- W4322753699 modified "2023-10-18" @default.
- W4322753699 title "Chromophore Structure in an Inactive State of a Novel Photosensor Protein Opn5L1: Resonance Raman Evidence for the Formation of a Deprotonated Adduct at the 11th Carbon Atom" @default.
- W4322753699 cites W1553520004 @default.
- W4322753699 cites W1969291352 @default.
- W4322753699 cites W1979013617 @default.
- W4322753699 cites W1981905570 @default.
- W4322753699 cites W1996468364 @default.
- W4322753699 cites W1997554177 @default.
- W4322753699 cites W1998270900 @default.
- W4322753699 cites W2005286485 @default.
- W4322753699 cites W2014290285 @default.
- W4322753699 cites W2016716827 @default.
- W4322753699 cites W2021676361 @default.
- W4322753699 cites W2028648518 @default.
- W4322753699 cites W2032182961 @default.
- W4322753699 cites W2033504728 @default.
- W4322753699 cites W2048162805 @default.
- W4322753699 cites W2051976570 @default.
- W4322753699 cites W2060153272 @default.
- W4322753699 cites W2061918195 @default.
- W4322753699 cites W2062704670 @default.
- W4322753699 cites W2073204622 @default.
- W4322753699 cites W2079504903 @default.
- W4322753699 cites W2080372016 @default.
- W4322753699 cites W2085160935 @default.
- W4322753699 cites W2100223135 @default.
- W4322753699 cites W2121570154 @default.
- W4322753699 cites W2138006564 @default.
- W4322753699 cites W2150792218 @default.
- W4322753699 cites W2331743987 @default.
- W4322753699 cites W2376955355 @default.
- W4322753699 cites W2794593985 @default.
- W4322753699 cites W2898486967 @default.
- W4322753699 cites W2933924315 @default.
- W4322753699 cites W3009104368 @default.
- W4322753699 cites W4206151110 @default.
- W4322753699 cites W4221096970 @default.
- W4322753699 doi "https://doi.org/10.1021/acs.jpcb.2c08780" @default.
- W4322753699 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36857774" @default.
- W4322753699 hasPublicationYear "2023" @default.
- W4322753699 type Work @default.
- W4322753699 citedByCount "0" @default.
- W4322753699 crossrefType "journal-article" @default.
- W4322753699 hasAuthorship W4322753699A5001842284 @default.
- W4322753699 hasAuthorship W4322753699A5008838993 @default.
- W4322753699 hasAuthorship W4322753699A5012095999 @default.
- W4322753699 hasAuthorship W4322753699A5019857622 @default.
- W4322753699 hasAuthorship W4322753699A5026074190 @default.
- W4322753699 hasAuthorship W4322753699A5033376423 @default.
- W4322753699 hasAuthorship W4322753699A5048875279 @default.
- W4322753699 hasAuthorship W4322753699A5065043759 @default.
- W4322753699 hasAuthorship W4322753699A5084042493 @default.
- W4322753699 hasAuthorship W4322753699A5086925320 @default.
- W4322753699 hasConcept C108204754 @default.
- W4322753699 hasConcept C109214941 @default.
- W4322753699 hasConcept C118629725 @default.
- W4322753699 hasConcept C119599485 @default.
- W4322753699 hasConcept C120665830 @default.
- W4322753699 hasConcept C121332964 @default.
- W4322753699 hasConcept C127413603 @default.
- W4322753699 hasConcept C139210041 @default.
- W4322753699 hasConcept C145148216 @default.
- W4322753699 hasConcept C147597530 @default.
- W4322753699 hasConcept C152365726 @default.
- W4322753699 hasConcept C155220765 @default.
- W4322753699 hasConcept C178790620 @default.
- W4322753699 hasConcept C180577832 @default.
- W4322753699 hasConcept C181199279 @default.
- W4322753699 hasConcept C185592680 @default.
- W4322753699 hasConcept C192468462 @default.
- W4322753699 hasConcept C202033177 @default.
- W4322753699 hasConcept C2779201268 @default.
- W4322753699 hasConcept C2780827179 @default.
- W4322753699 hasConcept C2781340169 @default.
- W4322753699 hasConcept C30028475 @default.
- W4322753699 hasConcept C40003534 @default.
- W4322753699 hasConcept C55493867 @default.
- W4322753699 hasConcept C68381374 @default.
- W4322753699 hasConcept C71240020 @default.
- W4322753699 hasConcept C75473681 @default.
- W4322753699 hasConcept C83581075 @default.
- W4322753699 hasConceptScore W4322753699C108204754 @default.
- W4322753699 hasConceptScore W4322753699C109214941 @default.
- W4322753699 hasConceptScore W4322753699C118629725 @default.
- W4322753699 hasConceptScore W4322753699C119599485 @default.