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- W4322758315 abstract "Amino acid metabolism by microorganisms is a new but important pathway for the formation of NDMA precursors in water. We investigated the properties of nitrosamine precursors produced through microbial metabolism of amino acids by polarity rapid assessment method and molecular weight fractionation by ultrafiltration method. The PRAM results showed that the positively charged fraction and the non-polar fraction accounted for most (45 %-79 % and 6 %-82 %, respectively) of the NDMA precursors. The MW fractionation results also indicate the dominant precursors had MWs <1 kDa or over 10 kDa. NDMA precursors produced through amino acid metabolism were identified and quantified. Dimethylamine, N-methyl-alanine and alanine methyl ester were produced during the metabolism of alanine and peptone. Together, N-methyl-alanine and dimethylamine averagely contributed 24 % (12 %-44 %) of the NDMA precursors in the alanine medium. The NDMA precursor formation pathway during alanine metabolism involves the methylation of alanine to form N-methyl-alanine and the decomposition of alanine anabolism products to form dimethylamine. Nitrosamine precursors are generally formed through anabolism or methylation, but biogenic amines or NH3 can be produced through catabolism before nitrosamine precursor synthesis. Microbial community analysis was performed and Ralstonia was found to be a likely key genus contributing to NDMA precursor formation during alanine metabolism." @default.
- W4322758315 created "2023-03-03" @default.
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- W4322758315 date "2023-05-01" @default.
- W4322758315 modified "2023-10-02" @default.
- W4322758315 title "Study on characteristic and mechanism involved in the formation of N-nitrosodimethylamine precursors during microbial metabolism of amino acids" @default.
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- W4322758315 doi "https://doi.org/10.1016/j.scitotenv.2023.162469" @default.
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