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• W4322766557 abstract "Abstract It is essential to establish a baseline in studies using stable isotopes to interpret trophic relationships across ecosystems and through time. Studies in freshwater ecosystems struggle to quantify baseline stable isotopes due to difficulties collecting representative samples, particularly from pelagic habitats. We assessed temporal and spatial variation in δ 13 C, δ 15 N, and δ 34 S in a commonly used pelagic baseline, seston ( n = 156), in Lake Erie to understand mechanisms that correlate with baseline stable isotope dynamics in large lakes. Seston contains a wide range of material which can confound stable isotope interpretation, and we examined the utility of element content and ratios to account for variation in sample source. Seston was collected in each of the three basins of Lake Erie from May to October in 2017–2019 at nearshore (<10 m depth) and offshore (>10 m depth) sites. General linear models were conducted on each stable isotope (δ 15 N, δ 13 C, and δ 34 S) and sample composition (variables: %N, %C, %S, C:N, C:S, and N:S) to assess how basin, month, and collection year influenced seston stable isotopes and composition. Sample composition (variables: %N, %C, %S, C:N, C:S, and N:S), which is rarely reported for organisms in stable isotope studies, was constant throughout the sample period with no temporal or spatial trends except for small variations in %C, C:N, C:S, and N:S. This indicated that the temporal and spatial trends observed within the stable isotopes were related to seasonal changes in system processes and plankton community dynamics, with few or minimal changes in the amount of detrital and inorganic material within seston. Values and trends of δ 15 N, δ 13 C, and δ 34 S in seston were comparable to those measured previously in Lake Erie and other Laurentian Great Lakes. All three isotopes increased from May to October of each sample year and varied spatially, δ 15 N was higher, δ 34 S was lower, and δ 13 C was the same in the west basin compared to the central and eastern basins of Lake Erie, which did not differ. These trends probably reflect seasonal changes in plankton community composition and nutrient cycling throughout the lake and are potentially linked to the presence of Microcystis blooms in the western basin during the late summer and autumn. Seston turns over quickly, as shown by the rapid changes in stable isotope values throughout the study, which confounds the investigation of stable isotopes in upper trophic levels, and especially in organisms that have slower tissue turnover and move throughout the lake seasonally. Additionally, the variable composition of seston (e.g., % C, % N, % S, C:N, C:S, N:S) necessitates analysing sample composition to determine the degree of abiotic (e.g., detritus, sediment, particulate organic matter) and biotic (e.g., phytoplankton, zooplankton) content in it." @default.
• W4322766557 created "2023-03-03" @default.
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• W4322766557 date "2023-03-02" @default.
• W4322766557 modified "2023-10-08" @default.
• W4322766557 title "Influence of spatial and temporal variation on establishing stable isotope baselines of <scp>δ¹⁵N</scp>, <scp>δ¹³C</scp>, and <scp>δ³⁴S</scp> in a large freshwater lake" @default.
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• W4322766557 doi "https://doi.org/10.1111/fwb.14065" @default.
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