Matches in SemOpenAlex for { <https://semopenalex.org/work/W4323021033> ?p ?o ?g. }
- W4323021033 endingPage "44" @default.
- W4323021033 startingPage "34" @default.
- W4323021033 abstract "Protein synthesis in Corynebacterium glutamicum is critical for applications in biotechnology and medicine. However, the use of C. glutamicum for protein production is limited by its low expression and aggregation. To overcome these limitations, a molecular chaperone plasmid system was developed in this study to improve the efficiency of recombinant protein synthesis in C. glutamicum. The effect of molecular chaperones on target protein synthesis (Single-chain variable fragment, Scfv) under three different promoter strengths was tested. In addition, the plasmid containing the molecular chaperone and target protein was verified for growth stability and plasmid stability. This expression model was further validated using two recombinant proteins, human interferon-beta (Hifn) and hirudin variant III (Rhv3). Finally, the Rhv3 protein was purified, and analysis of Rhv3 activity confirmed that the use of a molecular chaperone led to an improvement in test protein synthesis. Thus, the use of molecular chaperones is believed to will improve recombinant proteins synthesis in C. glutamicum." @default.
- W4323021033 created "2023-03-04" @default.
- W4323021033 creator A5000044349 @default.
- W4323021033 creator A5005756384 @default.
- W4323021033 creator A5008015122 @default.
- W4323021033 creator A5012152127 @default.
- W4323021033 creator A5013973657 @default.
- W4323021033 creator A5025431883 @default.
- W4323021033 creator A5056210370 @default.
- W4323021033 creator A5069724772 @default.
- W4323021033 date "2023-01-01" @default.
- W4323021033 modified "2023-09-30" @default.
- W4323021033 title "Enhanced production of recombinant proteins in <i>Corynebacterium glutamicum </i>using a molecular chaperone" @default.
- W4323021033 cites W1963770237 @default.
- W4323021033 cites W1968612981 @default.
- W4323021033 cites W1985161570 @default.
- W4323021033 cites W1993990155 @default.
- W4323021033 cites W2003791922 @default.
- W4323021033 cites W2036746284 @default.
- W4323021033 cites W2044780366 @default.
- W4323021033 cites W2055815318 @default.
- W4323021033 cites W2069573895 @default.
- W4323021033 cites W2072744250 @default.
- W4323021033 cites W2077054423 @default.
- W4323021033 cites W2080729278 @default.
- W4323021033 cites W2088550184 @default.
- W4323021033 cites W2097359698 @default.
- W4323021033 cites W2122833860 @default.
- W4323021033 cites W2133587231 @default.
- W4323021033 cites W2140373206 @default.
- W4323021033 cites W2141426247 @default.
- W4323021033 cites W2156573353 @default.
- W4323021033 cites W2214850930 @default.
- W4323021033 cites W2220864613 @default.
- W4323021033 cites W2473711714 @default.
- W4323021033 cites W2607798272 @default.
- W4323021033 cites W2734651258 @default.
- W4323021033 cites W2763427197 @default.
- W4323021033 cites W2780354290 @default.
- W4323021033 cites W2898576133 @default.
- W4323021033 cites W2913872601 @default.
- W4323021033 cites W2920948751 @default.
- W4323021033 cites W2921296962 @default.
- W4323021033 cites W2943019094 @default.
- W4323021033 cites W2945203504 @default.
- W4323021033 cites W2946196249 @default.
- W4323021033 cites W2986477971 @default.
- W4323021033 cites W2999551925 @default.
- W4323021033 cites W3004507833 @default.
- W4323021033 cites W3009080586 @default.
- W4323021033 cites W3010500104 @default.
- W4323021033 cites W3033720731 @default.
- W4323021033 cites W3081418767 @default.
- W4323021033 cites W3093855154 @default.
- W4323021033 doi "https://doi.org/10.2323/jgam.2022.10.002" @default.
- W4323021033 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36878578" @default.
- W4323021033 hasPublicationYear "2023" @default.
- W4323021033 type Work @default.
- W4323021033 citedByCount "0" @default.
- W4323021033 crossrefType "journal-article" @default.
- W4323021033 hasAuthorship W4323021033A5000044349 @default.
- W4323021033 hasAuthorship W4323021033A5005756384 @default.
- W4323021033 hasAuthorship W4323021033A5008015122 @default.
- W4323021033 hasAuthorship W4323021033A5012152127 @default.
- W4323021033 hasAuthorship W4323021033A5013973657 @default.
- W4323021033 hasAuthorship W4323021033A5025431883 @default.
- W4323021033 hasAuthorship W4323021033A5056210370 @default.
- W4323021033 hasAuthorship W4323021033A5069724772 @default.
- W4323021033 hasBestOaLocation W43230210331 @default.
- W4323021033 hasConcept C104317684 @default.
- W4323021033 hasConcept C142724271 @default.
- W4323021033 hasConcept C153911025 @default.
- W4323021033 hasConcept C185592680 @default.
- W4323021033 hasConcept C22744801 @default.
- W4323021033 hasConcept C2775962898 @default.
- W4323021033 hasConcept C2776260651 @default.
- W4323021033 hasConcept C2780430158 @default.
- W4323021033 hasConcept C33161422 @default.
- W4323021033 hasConcept C3675279 @default.
- W4323021033 hasConcept C40767141 @default.
- W4323021033 hasConcept C55493867 @default.
- W4323021033 hasConcept C71924100 @default.
- W4323021033 hasConcept C86803240 @default.
- W4323021033 hasConceptScore W4323021033C104317684 @default.
- W4323021033 hasConceptScore W4323021033C142724271 @default.
- W4323021033 hasConceptScore W4323021033C153911025 @default.
- W4323021033 hasConceptScore W4323021033C185592680 @default.
- W4323021033 hasConceptScore W4323021033C22744801 @default.
- W4323021033 hasConceptScore W4323021033C2775962898 @default.
- W4323021033 hasConceptScore W4323021033C2776260651 @default.
- W4323021033 hasConceptScore W4323021033C2780430158 @default.
- W4323021033 hasConceptScore W4323021033C33161422 @default.
- W4323021033 hasConceptScore W4323021033C3675279 @default.
- W4323021033 hasConceptScore W4323021033C40767141 @default.
- W4323021033 hasConceptScore W4323021033C55493867 @default.
- W4323021033 hasConceptScore W4323021033C71924100 @default.
- W4323021033 hasConceptScore W4323021033C86803240 @default.
- W4323021033 hasIssue "1" @default.