Matches in SemOpenAlex for { <https://semopenalex.org/work/W4323031536> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W4323031536 abstract "Neisseria gonorrhoeae (NG) is an urgent threat to antimicrobial resistance (AMR) worldwide. NG has acquired rapid resistance to all previously recommended treatments leaving ceftriaxone monotherapy as the first and last line of therapy for uncomplicated NG. The ability to rapidly determine susceptibility, which is currently nonexistent for NG, has been proposed as a strategy to preserve ceftriaxone by using alternative treatments. Herein, we used a DNA-intercalating dye in combination with NG-specific primers/probes to generate qPCR cycle threshold (Ct) values at different concentrations of 2 NG-relevant antimicrobials. Our proof of concept dual-antimicrobial logistic regression model based on the differential Ct measurements achieved an AUC of 0.93 with a categorical agreement for susceptibility of 84.6%. When surveying the performance against each antimicrobial separately, the model predicted 90% and 75% susceptible and resistant strains respectively to ceftriaxone and 66.7% and 83.3% susceptible and resistant strains respectively to ciprofloxacin. We further validated the model against the individual replicates and determined the accuracy of the model in classifying susceptibility agnostic of the inoculum size. We demonstrated a novel PCR-based approach to determine phenotypic ciprofloxacin and ceftriaxone susceptibility information for NG with reasonable accuracy in under 30 min, a significant improvement compared to the conventional method which takes 3 days." @default.
- W4323031536 created "2023-03-04" @default.
- W4323031536 creator A5015656765 @default.
- W4323031536 creator A5022016147 @default.
- W4323031536 creator A5026842930 @default.
- W4323031536 creator A5061361223 @default.
- W4323031536 creator A5066738610 @default.
- W4323031536 date "2023-03-01" @default.
- W4323031536 modified "2023-10-17" @default.
- W4323031536 title "Rapid molecular phenotypic antimicrobial susceptibility test for<i>Neisseria gonorrhoeae</i>based on propidium monoazide viability PCR" @default.
- W4323031536 cites W2054895460 @default.
- W4323031536 cites W2149869281 @default.
- W4323031536 cites W2157058280 @default.
- W4323031536 cites W2488728930 @default.
- W4323031536 cites W2508420029 @default.
- W4323031536 cites W2566245624 @default.
- W4323031536 cites W2758090759 @default.
- W4323031536 cites W2767382653 @default.
- W4323031536 cites W2779019546 @default.
- W4323031536 cites W2803778870 @default.
- W4323031536 cites W2808929849 @default.
- W4323031536 cites W2837231096 @default.
- W4323031536 cites W2917576966 @default.
- W4323031536 cites W2943099435 @default.
- W4323031536 cites W2949990847 @default.
- W4323031536 cites W2968926609 @default.
- W4323031536 cites W2972506161 @default.
- W4323031536 cites W3004298095 @default.
- W4323031536 cites W3014918727 @default.
- W4323031536 cites W3046985768 @default.
- W4323031536 cites W3048778046 @default.
- W4323031536 cites W3111151712 @default.
- W4323031536 cites W3124177967 @default.
- W4323031536 cites W3134149677 @default.
- W4323031536 cites W3187990339 @default.
- W4323031536 cites W3209107905 @default.
- W4323031536 cites W4210998108 @default.
- W4323031536 cites W4212923444 @default.
- W4323031536 cites W4220880554 @default.
- W4323031536 cites W4225985848 @default.
- W4323031536 doi "https://doi.org/10.1101/2023.03.01.530513" @default.
- W4323031536 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36909582" @default.
- W4323031536 hasPublicationYear "2023" @default.
- W4323031536 type Work @default.
- W4323031536 citedByCount "1" @default.
- W4323031536 countsByYear W43230315362023 @default.
- W4323031536 crossrefType "posted-content" @default.
- W4323031536 hasAuthorship W4323031536A5015656765 @default.
- W4323031536 hasAuthorship W4323031536A5022016147 @default.
- W4323031536 hasAuthorship W4323031536A5026842930 @default.
- W4323031536 hasAuthorship W4323031536A5061361223 @default.
- W4323031536 hasAuthorship W4323031536A5066738610 @default.
- W4323031536 hasBestOaLocation W43230315361 @default.
- W4323031536 hasConcept C2776520383 @default.
- W4323031536 hasConcept C2778512257 @default.
- W4323031536 hasConcept C2779166590 @default.
- W4323031536 hasConcept C4937899 @default.
- W4323031536 hasConcept C501593827 @default.
- W4323031536 hasConcept C86803240 @default.
- W4323031536 hasConcept C89423630 @default.
- W4323031536 hasConcept C94665300 @default.
- W4323031536 hasConceptScore W4323031536C2776520383 @default.
- W4323031536 hasConceptScore W4323031536C2778512257 @default.
- W4323031536 hasConceptScore W4323031536C2779166590 @default.
- W4323031536 hasConceptScore W4323031536C4937899 @default.
- W4323031536 hasConceptScore W4323031536C501593827 @default.
- W4323031536 hasConceptScore W4323031536C86803240 @default.
- W4323031536 hasConceptScore W4323031536C89423630 @default.
- W4323031536 hasConceptScore W4323031536C94665300 @default.
- W4323031536 hasLocation W43230315361 @default.
- W4323031536 hasLocation W43230315362 @default.
- W4323031536 hasLocation W43230315363 @default.
- W4323031536 hasOpenAccess W4323031536 @default.
- W4323031536 hasPrimaryLocation W43230315361 @default.
- W4323031536 hasRelatedWork W1988859956 @default.
- W4323031536 hasRelatedWork W2109230743 @default.
- W4323031536 hasRelatedWork W2207033334 @default.
- W4323031536 hasRelatedWork W2347868986 @default.
- W4323031536 hasRelatedWork W2350290426 @default.
- W4323031536 hasRelatedWork W2381344065 @default.
- W4323031536 hasRelatedWork W2393213968 @default.
- W4323031536 hasRelatedWork W2419175603 @default.
- W4323031536 hasRelatedWork W3207449696 @default.
- W4323031536 hasRelatedWork W4292552678 @default.
- W4323031536 isParatext "false" @default.
- W4323031536 isRetracted "false" @default.
- W4323031536 workType "article" @default.