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• W4323034966 abstract "Activation of hepatic stellate cells (HSCs) is considered as the central event during liver fibrosis.1, 2 H19 is an imprinted transcript and is upregulated during cholestatic liver fibrosis.3-6 However, few studies have explored the regulatory network of H19 during HSC activation. Here, we report that c-Jun, H19, miR-19a/b-3p and JNK1 form a feedback loop to promote HSC activation and hepatic fibrosis. Through fluorescence in situ hybridisation (FISH) and immunofluorescence (IF) assays, we observed the overexpression of H19 in both BDL-induced and CCL4-induced fibrotic livers (Figure S1) and the colocalisation of H19 with α-smooth muscle actin (α-SMA), a protein marker of activated HSCs (Figure 1B). In addition, H19 was upregulated in the transforming growth factor β1 (TGFβ1)-activated human HSC cell line LX-2 cells (Figure 1C). Upregulation of H19 further exacerbated LX-2 activation (Figure 1D–F and Figure S2A–C), whereas downregulation of H19 reduced the protein levels of α-SMA and collagen 1 in activated LX-2 cells (Figure S2D–F). To explore how H19 is upregulated in activated HSCs, we searched for upstream transcription factors (TFs) that potentially target the H19 gene (Figure 2A). c-Jun was found to be positively correlated with H19 (Figure S3) and has two potential binding sites at the promoter region of the H19 gene (Figure 2B). Luciferase reporter assays and ChIP assays verified the direct binding between the c-Jun protein and the H19 promoter region (Figure 2C,D). Increased c-Jun protein levels (Figure 2E–G) were also determined to be positively correlated with H19 levels in both BDL-induced and CCL4-induced fibrotic livers (Figure 2H). Overexpression of c-Jun increased H19 levels in LX-2 cells and vice versa (Figure 2I). These results suggested that c-Jun could trigger H19 transcription by binding to the promoter region during HSC activation. Given that H19 was mainly located in the cytoplasm of LX-2 cells (Figure 3A), we speculated that H19 might function as a competitive endogenous RNA (ceRNA). We screened eight miRNAs potentially sponged by H19 using bioinformatic analysis (Figure 3B). Among them, significant enrichment of miR-19a/b-3p was observed in the RNA samples pulled down by biotin-labelled H19 probe (Figure 3C and Figure S4A), indicating direct binding between miR-19a/b-3p and H19, which was also confirmed by a luciferase reporter assay (Figure 3D,E). However, H19 expression did not inhibit miR-19a/b-3p levels in LX-2 cells (Figure S5), suggesting that H19 might hijack miR-19a/b-3p to affect its function but not its expression. Although miR-19b has been reported to display an inhibitory effect in HSC-mediated fibrogenesis,7 the underlying mechanism is yet to be fully explained. We found that c-Jun N-terminal kinases 1 (JNK1) is potential target gene of miR-19a/b-3p. Up to eight bases of complementary pairing were found between the 3′ untranslated region (3′UTR) of JNK1 and the seed sequences of miR-19a/b-3p (Figure 3F). A luciferase reporter assay confirmed that miR-19a/b-3p directly targeted the JNK1 3′UTR (Figure 3G). RNA immunoprecipitation (RIP) assay detected that the AGO2 protein binds both miR-19a/b-3p and JNK1 mRNA (Figure 3H). In LX-2 cells, JNK1 protein expression was suppressed by miR-19a/b-3p overexpression and enhanced by miR-19a/b-3p knockdown (Figure S4B,C). In both BDL-induced and CCL4-induced fibrotic livers, the levels of JNK1 protein and its phosphorylation substrate,8, 9 and phosphorylated c-Jun (p-c-Jun) were significantly enhanced (Figure 3I and Figure S4D). JNK1 protein levels were inversely correlated with miR-19a/b-3p (Figure 3J). Previous studies have reported that JNK signaling is crucial for cell death, survival, differentiation, proliferation and tumorigenesis in the liver.10 The activation of JNK1 in HSCs is an essential fibrogenic event during hepatic fibrosis.8 Our data suggested that miR-19a/b-3p played an inhibitory role in liver fibrosis at least partly by negatively regulating JNK1 in HSCs. Next, we confirmed the downregulation of miR-19a/b-3p in clinical liver diseases by analysing sequencing data from the GEO database, including hepatitis C virus (HCV)-infected livers, hepatitis B virus (HBV)-infected livers, alcoholic steatohepatitis and nonalcoholic steatohepatitis (NASH) (Figure 4A). Reduced miR-19a/b-3p levels were also detected in the mouse fibrotic livers (Figure 4B). We further determined the inhibitory role of miR-19a/b-3p during hepatic fibrosis by lentivirus-mediated miR-19a/b-3p overexpression (Figure S6A). Compared to the control lentivirus groups, the miR-19a/b-3p-overexpressing lentivirus significantly restored the fibrotic phenotypes and the hepatic function indices in both BDL-induced and CCL4-induced livers (Figure 4C–E and Figure S6B,C). Herein, we identified a regulatory loop consisting of c-Jun/H19/miR-19a/b-3p/JNK1/c-Jun during HSC activation. In detail, when the liver is injured, H19 levels in HSCs were increased along with rapid propagation of HSCs. Previous research showed that both quiescent HSCs and activated HSCs could absorb H19 derived from cholangiocytes in cholestatic liver disease (Figure S7).6 Our study demonstrated that overexpression of H19 in HSCs, at least partly, also depends on c-Jun-triggered transcription during liver fibrosis. In turn, increased H19 in HSCs can relieve the inhibition of miR-19a/b-3p on JNK1 and thereby enable JNK1 to activate c-Jun, which will further promote the expression of H19. This cascade points out a new mechanism of HSC activation and transition from the initial activation state to the permanent activation state, and reinforces the value of epigenetic regulation during hepatic fibrosis. The in vivo results of lentivirus-mediated miR-19a/b-3p overexpression restoring the fibrotic phenotypes of both BDL and CCL4 mouse models also provide proof for miRNA-targeted therapies as novel therapeutic strategies for the prevention and treatment of liver fibrosis. We thank the following funding agencies: Natural Science Foundation of the Jiangsu Province (BK20200572), Postdoctoral Research Foundation of Jiangsu Province (1412000013), China Postdoctoral Science Foundation (No. 2018M642211) and Research Project of Jiangsu Health Committee (No. H2019046). The authors declare that they have no conflicts of interest. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article." @default.
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• W4323034966 date "2023-03-01" @default.
• W4323034966 modified "2023-10-01" @default.
• W4323034966 title "Identification of the c‐Jun/H19/miR‐19/JNK1 cascade during hepatic stellate cell activation" @default.
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• W4323034966 doi "https://doi.org/10.1002/ctm2.1106" @default.
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