Matches in SemOpenAlex for { <https://semopenalex.org/work/W4323036828> ?p ?o ?g. }
- W4323036828 abstract "Abstract Cell reprogramming involves time-intensive, costly processes that ultimately produce low numbers of reprogrammed cells of variable quality. By screening a range of polyacrylamide hydrogels (pAAm gels) of varying stiffness (1 kPA – 1.3 MPa) we found that a gel of medium stiffness significantly increases the overall number of reprogrammed cells by up to ten-fold with accelerated reprogramming kinetics, as compared to the standard Tissue Culture PolyStyrene (TCPS)-based protocol. We observe that though the gel improves both early and late phases of reprogramming, improvement in the late (reprogramming prone population maturation) phase is more pronounced and produces iPSCs having different characteristics and lower remnant transgene expression than those produced on TCPS. Comparative RNA-Seq analyses coupled with experimental validation reveals that modulation of Bone Morphogenic Protein (BMP) signalling by a novel reprogramming regulator, Phactr3, upregulated in the gel at an earliest time-point without the influence of transcription factors used for reprogramming, plays a crucial role in the improvement in the early reprogramming kinetics and overall reprogramming outcomes. This study provides new insights into the mechanism via which substrate stiffness modulates reprogramming kinetics and iPSC quality outcomes, opening new avenues for producing higher numbers of quality iPSCs or other reprogrammed cells at shorter timescales." @default.
- W4323036828 created "2023-03-04" @default.
- W4323036828 creator A5007328021 @default.
- W4323036828 creator A5012662669 @default.
- W4323036828 creator A5012942304 @default.
- W4323036828 creator A5020029453 @default.
- W4323036828 creator A5020230003 @default.
- W4323036828 creator A5025645526 @default.
- W4323036828 creator A5051164414 @default.
- W4323036828 creator A5066605681 @default.
- W4323036828 creator A5083371097 @default.
- W4323036828 date "2023-03-01" @default.
- W4323036828 modified "2023-10-18" @default.
- W4323036828 title "Substrate stiffness facilitates improved induced pluripotent stem cell production through modulation of both early and late phases of cell reprogramming" @default.
- W4323036828 cites W1721928266 @default.
- W4323036828 cites W1969015262 @default.
- W4323036828 cites W1994760449 @default.
- W4323036828 cites W2003315801 @default.
- W4323036828 cites W2004355850 @default.
- W4323036828 cites W2010672807 @default.
- W4323036828 cites W2017182831 @default.
- W4323036828 cites W2024438127 @default.
- W4323036828 cites W2032647105 @default.
- W4323036828 cites W2034093376 @default.
- W4323036828 cites W2037693206 @default.
- W4323036828 cites W2039986807 @default.
- W4323036828 cites W2040739802 @default.
- W4323036828 cites W2044830131 @default.
- W4323036828 cites W2047684973 @default.
- W4323036828 cites W2054066197 @default.
- W4323036828 cites W2058738554 @default.
- W4323036828 cites W2060613703 @default.
- W4323036828 cites W2060889734 @default.
- W4323036828 cites W2081205276 @default.
- W4323036828 cites W2082736420 @default.
- W4323036828 cites W2092613163 @default.
- W4323036828 cites W2100455753 @default.
- W4323036828 cites W2112664126 @default.
- W4323036828 cites W2126409708 @default.
- W4323036828 cites W2146978133 @default.
- W4323036828 cites W2148328620 @default.
- W4323036828 cites W2159814867 @default.
- W4323036828 cites W2169456326 @default.
- W4323036828 cites W2178456244 @default.
- W4323036828 cites W2180144971 @default.
- W4323036828 cites W2256191215 @default.
- W4323036828 cites W2273560121 @default.
- W4323036828 cites W2275366028 @default.
- W4323036828 cites W2434261529 @default.
- W4323036828 cites W2468655965 @default.
- W4323036828 cites W2479215566 @default.
- W4323036828 cites W2542225422 @default.
- W4323036828 cites W2546362712 @default.
- W4323036828 cites W2554038030 @default.
- W4323036828 cites W2577758672 @default.
- W4323036828 cites W2594273098 @default.
- W4323036828 cites W2602584217 @default.
- W4323036828 cites W2652333723 @default.
- W4323036828 cites W2752405877 @default.
- W4323036828 cites W2765444297 @default.
- W4323036828 cites W2767569544 @default.
- W4323036828 cites W2771828348 @default.
- W4323036828 cites W2782971933 @default.
- W4323036828 cites W2790448745 @default.
- W4323036828 cites W2791520226 @default.
- W4323036828 cites W2792957590 @default.
- W4323036828 cites W2796750766 @default.
- W4323036828 cites W2799633834 @default.
- W4323036828 cites W2802195803 @default.
- W4323036828 cites W2942799604 @default.
- W4323036828 cites W2944698121 @default.
- W4323036828 cites W2971278280 @default.
- W4323036828 cites W2974854894 @default.
- W4323036828 cites W2981198288 @default.
- W4323036828 cites W3088983895 @default.
- W4323036828 cites W3090442253 @default.
- W4323036828 cites W3102494221 @default.
- W4323036828 cites W3138927870 @default.
- W4323036828 cites W3166417300 @default.
- W4323036828 cites W4205553448 @default.
- W4323036828 cites W4224046406 @default.
- W4323036828 doi "https://doi.org/10.1101/2023.02.27.530366" @default.
- W4323036828 hasPublicationYear "2023" @default.
- W4323036828 type Work @default.
- W4323036828 citedByCount "0" @default.
- W4323036828 crossrefType "posted-content" @default.
- W4323036828 hasAuthorship W4323036828A5007328021 @default.
- W4323036828 hasAuthorship W4323036828A5012662669 @default.
- W4323036828 hasAuthorship W4323036828A5012942304 @default.
- W4323036828 hasAuthorship W4323036828A5020029453 @default.
- W4323036828 hasAuthorship W4323036828A5020230003 @default.
- W4323036828 hasAuthorship W4323036828A5025645526 @default.
- W4323036828 hasAuthorship W4323036828A5051164414 @default.
- W4323036828 hasAuthorship W4323036828A5066605681 @default.
- W4323036828 hasAuthorship W4323036828A5083371097 @default.
- W4323036828 hasBestOaLocation W43230368281 @default.
- W4323036828 hasConcept C104317684 @default.
- W4323036828 hasConcept C107459253 @default.
- W4323036828 hasConcept C127561419 @default.
- W4323036828 hasConcept C145103041 @default.