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- W4323075863 abstract "Abstract Transgene-free genome editing of plants in the T0 generation is highly desirable but challenging, especially in perennials and vegetatively propagated plants. Here, we investigated the co-editing strategy for generating transgene-free, gene-edited plants via Agrobacterium -mediated transient expression of cytosine base editor (CBE)/gRNA-Cas12a/crRNA-GFP in planta . Specifically, CBE/gRNA was used to base edit the ALS gene to confer resistance to herbicide chlorsulfuron as a selection marker, which has no negative effects on plant phenotypes; Cas12a/crRNA was used for editing genes(s) of interest; GFP was used for selecting transgene-free transformants. Using this approach, transgene-free genome-edited plants were efficiently generated for various genes (either individual or multiplex) in tomato, tobacco, potato, and citrus in the T0 generation. The biallelic/homozygous transgene-free mutation rates for target genes among herbicide-resistant transformants ranged from 8% to 50%. Whole genome sequencing further confirmed transgene-free and absence of off-target mutations in the edited plants. The co-editing strategy is efficient for generating transgene-free, genome-edited plants in the T0 generation, thus being a potent tool for plant genetic improvement." @default.
- W4323075863 created "2023-03-05" @default.
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- W4323075863 date "2023-03-02" @default.
- W4323075863 modified "2023-09-26" @default.
- W4323075863 title "Efficient transgene-free genome editing in plants in the T0 generation based on a co-editing strategy" @default.
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- W4323075863 doi "https://doi.org/10.1101/2023.03.02.530790" @default.
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