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• W4323256961 endingPage "108409" @default.
• W4323256961 startingPage "108409" @default.
• W4323256961 abstract "This study aimed to prepare a novel “self-regenerative” electrochemical biosensor by successively modifying gold nanoparticles, four-arm polyethylene glycol-NH2, and NH2-MIL-53 (Al) (MOF) on the glassy carbon electrode interface. A hairpin G-triplex-mediated DNA (G3 probe) as a part of the mycoplasma ovine pneumonia (MO) gene was loosely adsorbed to MOF. Based on the mechanism of hybridization induction, the G3 probe could effectively detach from the MOF only after introducing the target DNA. Subsequently, its guanine-rich nucleic acid sequences were exposed to solution of methylene blue. As a result, the diffusion current of the sensor system showed a sharp decline. The developed biosensor showed excellent selectivity, and the concentration of target DNA exhibited a good correlation in the range 10–10 to 10–6 M with a detection limit of 1.00 pM (S/N = 3), even in 10% goat serum. Most interestingly, this biosensor interface automatically started the regeneration program. Moreover, regeneration could be effectively achieved at least seven times, and the recovery rate of the electrode interface and sensing efficiency was up to 90%. Additionally, this platform could be used for other clinical assays in various systems by simply changing the DNA sequence of the probe." @default.
• W4323256961 created "2023-03-06" @default.
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• W4323256961 date "2023-08-01" @default.
• W4323256961 modified "2023-10-01" @default.
• W4323256961 title "Construction of a novel “self-regenerative” electrochemical biosensor based on metal–organic frameworks and its application to the detection of Mycoplasma ovine pneumonia" @default.
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• W4323256961 doi "https://doi.org/10.1016/j.bioelechem.2023.108409" @default.
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• W4323256961 hasPublicationYear "2023" @default.
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