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• W4323267235 abstract "Theiler's murine encephalomyelitis virus (TMEV) is a single-stranded RNA virus that causes encephalitis followed by chronic demyelination in SJL mice and spontaneous seizures in C57BL/6 mice. Since earlier studies indicated a critical role of type I interferon (IFN-I) signaling in the control of viral replication in the central nervous system (CNS), mouse strain-specific differences in pathways induced by the IFN-I receptor (IFNAR) might determine the outcome of TMEV infection.Data of RNA-seq analysis and immunohistochemistry were used to compare the gene and protein expression of IFN-I signaling pathway members between mock- and TMEV-infected SJL and C57BL/6 mice at 4, 7 and 14 days post-infection (dpi). To address the impact of IFNAR signaling in selected brain-resident cell types, conditional knockout mice with an IFNAR deficiency in cells of the neuroectodermal lineage (NesCre±IFNARfl/fl), neurons (Syn1Cre±IFNARfl/fl), astrocytes (GFAPCre±IFNARfl/fl), and microglia (Sall1CreER±IFNARfl/fl) on a C57BL/6 background were tested. PCR and an immunoassay were used to quantify TMEV RNA and cytokine and chemokine expression in their brain at 4 dpi.RNA-seq analysis revealed upregulation of most ISGs in SJL and C57BL/6 mice, but Ifi202b mRNA transcripts were only increased in SJL and Trim12a only in C57BL/6 mice. Immunohistochemistry showed minor differences in ISG expression (ISG15, OAS, PKR) between both mouse strains. While all immunocompetent Cre-negative control mice and the majority of mice with IFNAR deficiency in neurons or microglia survived until 14 dpi, lack of IFNAR expression in all cells (IFNAR-/-), neuroectodermal cells, or astrocytes induced lethal disease in most of the analyzed mice, which was associated with unrestricted viral replication. NesCre±IFNARfl/fl mice showed more Ifnb1, Tnfa, Il6, Il10, Il12b and Ifng mRNA transcripts than Cre-/-IFNARfl/fl mice. IFNAR-/- mice also demonstrated increased IFN-α, IFN-β, IL1-β, IL-6, and CXCL-1 protein levels, which highly correlated with viral load.Ifi202b and Trim12a expression levels likely contribute to mouse strain-specific susceptibility to TMEV-induced CNS lesions. Restriction of viral replication is strongly dependent on IFNAR signaling of neuroectodermal cells, which also controls the expression of key pro- and anti-inflammatory cytokines during viral brain infection." @default.
• W4323267235 created "2023-03-06" @default.
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• W4323267235 date "2023-03-05" @default.
• W4323267235 modified "2023-10-09" @default.
• W4323267235 title "IFNAR signaling of neuroectodermal cells is essential for the survival of C57BL/6 mice infected with Theiler’s murine encephalomyelitis virus" @default.
• W4323267235 cites W1485498049 @default.
• W4323267235 cites W1577577364 @default.
• W4323267235 cites W1659069578 @default.
• W4323267235 cites W1761323037 @default.
• W4323267235 cites W1913184799 @default.
• W4323267235 cites W1914304328 @default.
• W4323267235 cites W1923330562 @default.
• W4323267235 cites W1970160145 @default.
• W4323267235 cites W1973268693 @default.
• W4323267235 cites W1978531451 @default.
• W4323267235 cites W1980444303 @default.
• W4323267235 cites W1982004491 @default.
• W4323267235 cites W1983150631 @default.
• W4323267235 cites W1990193319 @default.
• W4323267235 cites W1997380254 @default.
• W4323267235 cites W1998488372 @default.
• W4323267235 cites W1999610187 @default.
• W4323267235 cites W2000031391 @default.
• W4323267235 cites W2000177767 @default.
• W4323267235 cites W2000728022 @default.
• W4323267235 cites W2001963721 @default.
• W4323267235 cites W2003772658 @default.
• W4323267235 cites W2003877200 @default.
• W4323267235 cites W2004829503 @default.
• W4323267235 cites W2005873273 @default.
• W4323267235 cites W2006882008 @default.
• W4323267235 cites W2009011593 @default.
• W4323267235 cites W2009904875 @default.
• W4323267235 cites W2014068440 @default.
• W4323267235 cites W2016749309 @default.
• W4323267235 cites W2016899230 @default.
• W4323267235 cites W2021661809 @default.
• W4323267235 cites W2021951044 @default.
• W4323267235 cites W2022970081 @default.
• W4323267235 cites W2027440189 @default.
• W4323267235 cites W2031913105 @default.
• W4323267235 cites W2032235021 @default.
• W4323267235 cites W2033856426 @default.
• W4323267235 cites W2035308998 @default.
• W4323267235 cites W2035509260 @default.
• W4323267235 cites W2037012882 @default.
• W4323267235 cites W2037897155 @default.
• W4323267235 cites W2037971991 @default.
• W4323267235 cites W2039423387 @default.
• W4323267235 cites W2042095978 @default.
• W4323267235 cites W2042433752 @default.
• W4323267235 cites W2043648764 @default.
• W4323267235 cites W2046413321 @default.
• W4323267235 cites W2047882003 @default.
• W4323267235 cites W2049592181 @default.
• W4323267235 cites W2051962517 @default.
• W4323267235 cites W2058135612 @default.
• W4323267235 cites W2059901677 @default.
• W4323267235 cites W2060926084 @default.
• W4323267235 cites W2061742276 @default.
• W4323267235 cites W2062623649 @default.
• W4323267235 cites W2063082801 @default.
• W4323267235 cites W2067610638 @default.
• W4323267235 cites W2072766433 @default.
• W4323267235 cites W2077808289 @default.
• W4323267235 cites W2080867472 @default.
• W4323267235 cites W2081232744 @default.
• W4323267235 cites W2089077517 @default.
• W4323267235 cites W2100155537 @default.
• W4323267235 cites W2104167354 @default.
• W4323267235 cites W2104819392 @default.
• W4323267235 cites W2106090998 @default.
• W4323267235 cites W2111807233 @default.
• W4323267235 cites W2116056936 @default.
• W4323267235 cites W2118521277 @default.
• W4323267235 cites W2123986022 @default.
• W4323267235 cites W2124034233 @default.
• W4323267235 cites W2124667416 @default.
• W4323267235 cites W2140729960 @default.
• W4323267235 cites W2149278532 @default.
• W4323267235 cites W2150098469 @default.
• W4323267235 cites W2152305936 @default.
• W4323267235 cites W2161530562 @default.
• W4323267235 cites W2164832593 @default.
• W4323267235 cites W2168876790 @default.
• W4323267235 cites W2177325722 @default.
• W4323267235 cites W2179438025 @default.

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