Matches in SemOpenAlex for { <https://semopenalex.org/work/W4323350416> ?p ?o ?g. }
- W4323350416 abstract "Abstract Gene regulation plays essential roles in all multicellular organisms, allowing for different specialized tissue types to be generated from a complex genome. Heterochromatin-driven gene repression, associated with a physical compaction of the genome, is a pathway involving core components that are conserved from yeast to human. Posttranslational modification of chromatin is a critical component of gene regulation. Specifically, tri-methylation of the nucleosome component histone 3 at lysine 9 (H3K9me3) is a key feature of this pathway along with the hallmark heterochromatin protein 1 (HP1). Histone methyltransferases are recruited by HP1 to deposit H3K9me3 marks which nucleate and recruit more HP1 in a process that spreads from the targeting site to signal for gene repression. One of the enzymes recruited is SETDB1, a methyltransferase which putatively catalyzes posttranslational methylation marks on H3K9. To better understand the contribution of SETDB1 in heterochromatin formation, we downregulated SETDB1 through knockdown by a dCas9-KRAB system and examined heterochromatin formation in a chromatin in vivo assay (CiA-Oct4). We studied the contribution of SETDB1 to heterochromatin formation kinetics in a developmentally crucial locus, Oct4. Our data demonstrate that SETDB1 reduction led to a delay in both gene silencing and in H3K9me3 accumulation. Importantly, SETDB1 knockdown to a ∼50% level did not stop heterochromatin formation completely. Particle-based Monte Carlo simulations in 3D space with explicit representation of key molecular processes enabled the elucidation of how SETDB1 downregulation affects the individual molecular processes underlying heterochromatin formation." @default.
- W4323350416 created "2023-03-08" @default.
- W4323350416 creator A5018675233 @default.
- W4323350416 creator A5022577795 @default.
- W4323350416 creator A5055457252 @default.
- W4323350416 creator A5065677103 @default.
- W4323350416 creator A5074788536 @default.
- W4323350416 date "2023-03-07" @default.
- W4323350416 modified "2023-10-18" @default.
- W4323350416 title "Relationship between lysine methyltransferase levels and heterochromatin gene repression in living cells and in silico" @default.
- W4323350416 cites W1509466986 @default.
- W4323350416 cites W1603793127 @default.
- W4323350416 cites W1965262464 @default.
- W4323350416 cites W1966976494 @default.
- W4323350416 cites W1977621555 @default.
- W4323350416 cites W1979667448 @default.
- W4323350416 cites W1986606329 @default.
- W4323350416 cites W1989456683 @default.
- W4323350416 cites W1997388726 @default.
- W4323350416 cites W2000342325 @default.
- W4323350416 cites W2012598468 @default.
- W4323350416 cites W2012935454 @default.
- W4323350416 cites W2015757633 @default.
- W4323350416 cites W2017050785 @default.
- W4323350416 cites W2018113993 @default.
- W4323350416 cites W2020363669 @default.
- W4323350416 cites W2027933092 @default.
- W4323350416 cites W2029733458 @default.
- W4323350416 cites W2029903254 @default.
- W4323350416 cites W2040891039 @default.
- W4323350416 cites W2044917002 @default.
- W4323350416 cites W2045619208 @default.
- W4323350416 cites W2055327776 @default.
- W4323350416 cites W2055790209 @default.
- W4323350416 cites W2060446375 @default.
- W4323350416 cites W2063982921 @default.
- W4323350416 cites W2065529250 @default.
- W4323350416 cites W2066018141 @default.
- W4323350416 cites W2067487392 @default.
- W4323350416 cites W2074329146 @default.
- W4323350416 cites W2079471807 @default.
- W4323350416 cites W2081007008 @default.
- W4323350416 cites W2094221767 @default.
- W4323350416 cites W2094772864 @default.
- W4323350416 cites W2098497185 @default.
- W4323350416 cites W2105073128 @default.
- W4323350416 cites W2105617035 @default.
- W4323350416 cites W2106903510 @default.
- W4323350416 cites W2107557989 @default.
- W4323350416 cites W2111801210 @default.
- W4323350416 cites W2114088699 @default.
- W4323350416 cites W2119036640 @default.
- W4323350416 cites W2120749266 @default.
- W4323350416 cites W2126151185 @default.
- W4323350416 cites W2130350241 @default.
- W4323350416 cites W2130907138 @default.
- W4323350416 cites W2134224251 @default.
- W4323350416 cites W2135318926 @default.
- W4323350416 cites W2141452262 @default.
- W4323350416 cites W2142042705 @default.
- W4323350416 cites W2145750252 @default.
- W4323350416 cites W2148652257 @default.
- W4323350416 cites W2151026121 @default.
- W4323350416 cites W2160051211 @default.
- W4323350416 cites W2162618509 @default.
- W4323350416 cites W2163560954 @default.
- W4323350416 cites W2166164324 @default.
- W4323350416 cites W2166759130 @default.
- W4323350416 cites W2167495362 @default.
- W4323350416 cites W2180790011 @default.
- W4323350416 cites W2204875887 @default.
- W4323350416 cites W2252333393 @default.
- W4323350416 cites W2279123229 @default.
- W4323350416 cites W2288928506 @default.
- W4323350416 cites W2332009504 @default.
- W4323350416 cites W2338344824 @default.
- W4323350416 cites W2343776142 @default.
- W4323350416 cites W2411128394 @default.
- W4323350416 cites W2468725635 @default.
- W4323350416 cites W2599703488 @default.
- W4323350416 cites W2724956132 @default.
- W4323350416 cites W2741244157 @default.
- W4323350416 cites W2743033273 @default.
- W4323350416 cites W2801872537 @default.
- W4323350416 cites W2810076504 @default.
- W4323350416 cites W2884613836 @default.
- W4323350416 cites W2891961305 @default.
- W4323350416 cites W2903091331 @default.
- W4323350416 cites W2907110015 @default.
- W4323350416 cites W2944481848 @default.
- W4323350416 cites W2945583986 @default.
- W4323350416 cites W2947887186 @default.
- W4323350416 cites W2948487481 @default.
- W4323350416 cites W2952746258 @default.
- W4323350416 cites W2956020134 @default.
- W4323350416 cites W2973813155 @default.
- W4323350416 cites W2980597982 @default.
- W4323350416 cites W2985591769 @default.
- W4323350416 cites W3007432749 @default.
- W4323350416 cites W3164760726 @default.