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- W4323517773 abstract "A novel chemoenzymatic synthesis of UDP-N-acetyl-α-d-galactosamine starting from uridine 5′-monophosphate (UMP) and sucrose is reported. In an enzymatic repetitive batch mode UDP-glucose was generated in situ from UMP and sucrose by the combination of nucleoside monophosphate kinase (EC 2.7.7.4) and sucrose synthase (EC 2.4.1.13). The transfer of UMP from UDP-glucose by galactose-1-phosphate uridyltransferase (EC 2.7.7.12) yielded UDP-α-d-galactosamine. The equilibrium of the synthesis was forced to the product side by the addition of phosphoglucomutase (EC 2.7.5.1) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49). Pyruvate kinase (EC 2.7.1.40) and lactate dehydrogenase (EC 1.1.1.27) were used to regenerate UTP and the cofactor NAD. The yield for the enzymatic step was 42%. Finally, UDP-α-d-galactosamine was acetylated chemically with N-acetoxysuccinimide. Product isolation was accomplished by anion-exchange chromatography and gel filtration. The overall yield was 34% and 82 mg UDP-N-acetyl-α-d-galactosamine were isolated." @default.
- W4323517773 created "2023-03-09" @default.
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- W4323517773 date "1963-01-01" @default.
- W4323517773 modified "2023-09-26" @default.
- W4323517773 title "N-Acetyl-galactosamine α-1-phosphate and uridine-diphospho-N-acetyl galactosamine" @default.
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- W4323517773 doi "https://doi.org/10.1016/0926-6550(63)90317-7" @default.
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