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- W4323543513 abstract "ABSTRACT P. malariae is found worldwide and causes chronic parasitism in its human hosts. We developed a P. malariae (Pm) diagnostic assay that uses rapid, isothermal recombinase polymerase amplification (RPA) and lateral-flow-strip detection. Using 18S rRNA plasmid DNA, the assay demonstrates a detection limit of 10 copies /µL (∼1.7 genome equivalents) and 100% analytical specificity. Testing in field samples showed 95% clinical sensitivity and 88% specificity compared to qPCR. Total assay time was 35 minutes. Combined with simplified DNA extraction methods, the assay has potential for future field-deployable point-of-care use to detect a parasite species that remains largely undiagnosed." @default.
- W4323543513 created "2023-03-09" @default.
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- W4323543513 date "2023-03-01" @default.
- W4323543513 modified "2023-10-14" @default.
- W4323543513 title "Detection of<i>P. malariae</i>using a new rapid isothermal amplification lateral flow assay" @default.
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- W4323543513 doi "https://doi.org/10.1101/2023.02.26.23286371" @default.
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