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• W4323656266 abstract "We report the first bioconjugation of Au25 nanocluster to a monoclonal antibody at scarcely exposed tryptophan (Trp) residues toward the development of high-resolution probes for cryogenic electron microscopy (cryo-EM) and tomography (cryo-ET). To achieve this, we improved the Trp-selective bioconjugation using hydroxylamine (ABNOH) reagents instead of previously developed N-oxyl radicals (ABNO). This new protocol allowed for the application of Trp-selective bioconjugation to acid-sensitive proteins such as antibodies. We found that a two-step procedure utilizing first Trp-selective bioconjugation for the introduction of azide groups to the protein and then strain-promoted azide-alkyne cycloaddition (SPAAC) to attach a bicyclononyne (BCN)-presenting redox-sensitive Au25 nanocluster was essential for a scalable procedure. Covalent labeling of the antibody with gold nanoclusters was confirmed by various analytical methods, including cryo-EM analysis of the Au25 nanocluster conjugates." @default.
• W4323656266 created "2023-03-10" @default.
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• W4323656266 date "2023-03-09" @default.
• W4323656266 modified "2023-10-18" @default.
• W4323656266 title "Bioconjugation of Au₂₅ Nanocluster to Monoclonal Antibody at Tryptophan" @default.
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• W4323656266 doi "https://doi.org/10.1021/acs.bioconjchem.3c00069" @default.
• W4323656266 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36893358" @default.
• W4323656266 hasPublicationYear "2023" @default.
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