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- W4323670060 endingPage "110225" @default.
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- W4323670060 abstract "L-Phosphinothricin (L-PPT) is the effective constituent in racemic PPT (a high-efficiency and broad-spectrum herbicide), and the exploitation of green and sustainable synthesis route for L-PPT has always been the focus in pesticide industry. In recent years, one-pot, two-step enzyme-mediated cascade strategy is a mainstream pathway to obtain L-PPT. Herein, RgDAAO and BsLeuDH were applied to expand one-pot, two-step process. Notably, a NADH-dependent leucine dehydrogenase from Bacillus subtilis (BsLeuDH) was firstly characterized and attempted to generate L-PPT, achieving an excellent enantioselectivity (99.9% ee). Meanwhile, a formate dehydrogenase from Pichia pastoris (PpFDH) was utilized to implement NADH cofactor regeneration and only CO2 was by-product. Sufficient amount of the corresponding keto acid precursor PPO was obtained by oxidation of D-PPT relying on a D-amino acid oxidase from Rhodotorula gracilis (RgDAAO) with content conversion (46.1%). L-PPT was ultimately prepared from racemized PPT via oxidative deamination catalyzed by RgDAAO and reductive amination catalyzed by BsLeuDH, achieving 80.3% overall yield and > 99.9% ee value." @default.
- W4323670060 created "2023-03-10" @default.
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- W4323670060 date "2023-05-01" @default.
- W4323670060 modified "2023-10-16" @default.
- W4323670060 title "A novel NADH-dependent leucine dehydrogenase for multi-step cascade synthesis of L-phosphinothricin" @default.
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- W4323670060 doi "https://doi.org/10.1016/j.enzmictec.2023.110225" @default.
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