Matches in SemOpenAlex for { <https://semopenalex.org/work/W4323818910> ?p ?o ?g. }
- W4323818910 abstract "Age-related macular degeneration (AMD) is a debilitating retinal disorder in aging populations. It is widely believed that dysfunction of the retinal pigmented epithelium (RPE) is a key pathobiological event in AMD. To understand the mechanisms that lead to RPE dysfunction, mouse models can be utilized by researchers. It has been established by previous studies that mice can develop RPE pathologies, some of which are observed in the eyes of individuals diagnosed with AMD. Here, we describe a phenotyping protocol to assess RPE pathologies in mice. This protocol includes the preparation and evaluation of retinal cross-sections using light microscopy and transmission electron microscopy, as well as that of RPE flat mounts by confocal microscopy. We detail the common types of murine RPE pathologies observed by these techniques and ways to quantify them through unbiased methods for statistical testing. As proof of concept, we use this RPE phenotyping protocol to quantify the RPE pathologies observed in mice overexpressing transmembrane protein 135 (Tmem135) and aged wild-type C57BL/6J mice. The main goal of this protocol is to present standard RPE phenotyping methods with unbiased quantitative assessments for scientists using mouse models of AMD." @default.
- W4323818910 created "2023-03-11" @default.
- W4323818910 creator A5003171029 @default.
- W4323818910 creator A5013830528 @default.
- W4323818910 creator A5042881885 @default.
- W4323818910 creator A5051860617 @default.
- W4323818910 creator A5070186809 @default.
- W4323818910 creator A5082640513 @default.
- W4323818910 date "2023-03-10" @default.
- W4323818910 modified "2023-10-08" @default.
- W4323818910 title "A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration" @default.
- W4323818910 cites W2007014682 @default.
- W4323818910 cites W2022073599 @default.
- W4323818910 cites W2028550618 @default.
- W4323818910 cites W2038364869 @default.
- W4323818910 cites W2043444804 @default.
- W4323818910 cites W2045620761 @default.
- W4323818910 cites W2072082451 @default.
- W4323818910 cites W2073078178 @default.
- W4323818910 cites W2081232564 @default.
- W4323818910 cites W2082578764 @default.
- W4323818910 cites W2094971722 @default.
- W4323818910 cites W2104426232 @default.
- W4323818910 cites W2115932454 @default.
- W4323818910 cites W2127507482 @default.
- W4323818910 cites W2160226180 @default.
- W4323818910 cites W2275799577 @default.
- W4323818910 cites W2412346009 @default.
- W4323818910 cites W2523178255 @default.
- W4323818910 cites W2783283549 @default.
- W4323818910 cites W2792284163 @default.
- W4323818910 cites W2794100172 @default.
- W4323818910 cites W2799426743 @default.
- W4323818910 cites W2801804301 @default.
- W4323818910 cites W2889983606 @default.
- W4323818910 cites W2898549572 @default.
- W4323818910 cites W2903438898 @default.
- W4323818910 cites W2914052007 @default.
- W4323818910 cites W2959132662 @default.
- W4323818910 cites W2966044133 @default.
- W4323818910 cites W3007934773 @default.
- W4323818910 cites W3044033155 @default.
- W4323818910 cites W3093204350 @default.
- W4323818910 cites W3104313195 @default.
- W4323818910 cites W3122999042 @default.
- W4323818910 cites W3193777758 @default.
- W4323818910 cites W3196897371 @default.
- W4323818910 cites W3213284275 @default.
- W4323818910 cites W4229011566 @default.
- W4323818910 cites W4285023693 @default.
- W4323818910 cites W4292367342 @default.
- W4323818910 doi "https://doi.org/10.3791/64927" @default.
- W4323818910 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36971449" @default.
- W4323818910 hasPublicationYear "2023" @default.
- W4323818910 type Work @default.
- W4323818910 citedByCount "0" @default.
- W4323818910 crossrefType "journal-article" @default.
- W4323818910 hasAuthorship W4323818910A5003171029 @default.
- W4323818910 hasAuthorship W4323818910A5013830528 @default.
- W4323818910 hasAuthorship W4323818910A5042881885 @default.
- W4323818910 hasAuthorship W4323818910A5051860617 @default.
- W4323818910 hasAuthorship W4323818910A5070186809 @default.
- W4323818910 hasAuthorship W4323818910A5082640513 @default.
- W4323818910 hasConcept C118487528 @default.
- W4323818910 hasConcept C142724271 @default.
- W4323818910 hasConcept C169760540 @default.
- W4323818910 hasConcept C2776403814 @default.
- W4323818910 hasConcept C2778024200 @default.
- W4323818910 hasConcept C2779178360 @default.
- W4323818910 hasConcept C2780827179 @default.
- W4323818910 hasConcept C2781040256 @default.
- W4323818910 hasConcept C71924100 @default.
- W4323818910 hasConcept C86803240 @default.
- W4323818910 hasConcept C95444343 @default.
- W4323818910 hasConceptScore W4323818910C118487528 @default.
- W4323818910 hasConceptScore W4323818910C142724271 @default.
- W4323818910 hasConceptScore W4323818910C169760540 @default.
- W4323818910 hasConceptScore W4323818910C2776403814 @default.
- W4323818910 hasConceptScore W4323818910C2778024200 @default.
- W4323818910 hasConceptScore W4323818910C2779178360 @default.
- W4323818910 hasConceptScore W4323818910C2780827179 @default.
- W4323818910 hasConceptScore W4323818910C2781040256 @default.
- W4323818910 hasConceptScore W4323818910C71924100 @default.
- W4323818910 hasConceptScore W4323818910C86803240 @default.
- W4323818910 hasConceptScore W4323818910C95444343 @default.
- W4323818910 hasIssue "193" @default.
- W4323818910 hasLocation W43238189101 @default.
- W4323818910 hasLocation W43238189102 @default.
- W4323818910 hasOpenAccess W4323818910 @default.
- W4323818910 hasPrimaryLocation W43238189101 @default.
- W4323818910 hasRelatedWork W1592825584 @default.
- W4323818910 hasRelatedWork W1977015089 @default.
- W4323818910 hasRelatedWork W2052947772 @default.
- W4323818910 hasRelatedWork W2081118860 @default.
- W4323818910 hasRelatedWork W2081998461 @default.
- W4323818910 hasRelatedWork W2413042731 @default.
- W4323818910 hasRelatedWork W2416017562 @default.
- W4323818910 hasRelatedWork W3202892161 @default.
- W4323818910 hasRelatedWork W3203428352 @default.
- W4323818910 hasRelatedWork W4360991425 @default.