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- W4323857515 abstract "ABSTRACT Potato brown rot, caused by Ralstonia solanacearum , is one of the most destructive diseases of potatoes. The pathogen could hide in the tuber, leading to the rotting tubers. However, few mechanisms of pathogenesis in tubers caused by brown rot were reported. Here, we identified a highly virulent type III effector RipBH, which is not only required for the pathogenesis of potato brown rot but also displays strong cell toxicity in yeast and tobacco. We found RipBH is a novel structural cysteine protease with a large ankyrin repeat domain that contains 10 ankyrin repeats, we named it as an ankyrin cysteine protease. Biochemical analysis showed that all the ankyrin repeats are required for virulence, and the first five ankyrin repeats are indispensable for auto-cleavage site recognition. Further analysis showed that RipBH triggered autophagy-associated cell death. The ankyrin cysteine protease effector existed extensively in plant and animal pathogens suggesting the ankyrin cysteine protease effectors are functionally essential for pathogen pathogenesis. Our study enhances our understanding of this type of cysteine protease and illustrates the pathogenesis of cysteine protease in potato brown rot." @default.
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- W4323857515 date "2023-03-09" @default.
- W4323857515 modified "2023-10-16" @default.
- W4323857515 title "A toxic ankyrin cysteine protease effector RipBH of brown rot triggered autophagy-associated cell death" @default.
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- W4323857515 doi "https://doi.org/10.1101/2023.03.08.531657" @default.
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