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• W4323913997 abstract "Processing of newly synthesized polypeptides is essential for protein homeostasis and cell viability. In bacteria and eukaryotic organelles, all proteins are synthesized with formylmethionine at their N-terminus. As the nascent peptide emerges from the ribosome during translation, the formyl group is removed by peptide deformylase (PDF), an enzyme that belongs to the family of ribosome-associated protein biogenesis factors (RPBs). Because PDF is essential in bacteria but not in humans (except for the PDF homolog acting in mitochondria), the bacterial enzyme is a promising antimicrobial drug target. While much of the mechanistic work on PDF was carried out using model peptides in solution, understanding the mechanism of PDF in cells and developing effective PDF inhibitors requires experiments with its native cellular substrates, i.e., ribosome-nascent chain complexes. Here, we describe protocols to purify PDF from Escherichia coli and to test its deformylation activity on the ribosome in multiple-turnover and single-round kinetic regimes as well as in binding assays. These protocols can be used to test PDF inhibitors, to study the peptide specificity of PDF and its interplay with other RPBs, as well as to compare the activity and specificity of bacterial and mitochondrial PDFs." @default.
• W4323913997 created "2023-03-12" @default.
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• W4323913997 date "2023-01-01" @default.
• W4323913997 modified "2023-10-18" @default.
• W4323913997 title "Deformylation of nascent peptide chains on the ribosome" @default.
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• W4323913997 doi "https://doi.org/10.1016/bs.mie.2023.02.010" @default.
• W4323913997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37230593" @default.
• W4323913997 hasPublicationYear "2023" @default.
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