FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4324017502> ?p ?o ?g. }

• W4324017502 endingPage "e0282963" @default.
• W4324017502 startingPage "e0282963" @default.
• W4324017502 abstract "Ninety-one elastase-producing bacterial isolates were recovered from different localities of the Eastern Province of Saudi Arabia. Elastase from the best isolate Priestia megaterium gasm32, from luncheon samples was purified to electrophoretic homogeneity using DEAE-Sepharose CL-6B and Sephadex G-100 chromatographic techniques. The recovery was 17.7%, the purification fold was 11.7 x , and the molecular mass was 30 kDa. Enzymatic activity was highly repressed by Ba 2+ and almost completely lost by EDTA, but it was greatly stimulated by Cu 2+ ions, suggesting a metalloprotease type. The enzyme was stable at 45°C and pH 6.0–10.0 for 2 hours. Ca 2+ ions considerably enhanced the stability of the heat-treated enzyme. The V max and K m against the synthetic substrate elastin–Congo red were 6.03 mg/mL, and 8.82 U/mg, respectively. Interestingly, the enzyme showed potent antibacterial activity against many bacterial pathogens. Under SEM, most bacterial cells showed loss of integrity, damage, and perforation. SEM micrographs also showed a time-dependent gradual breakdown of elastin fibers exposed to elastase. After 3 hours, intact elastin fibers disappeared, leaving irregular pieces. Given these good features, this elastase may be a promising candidate for treating damaged skin fibers with the inhibition of contaminating bacteria." @default.
• W4324017502 created "2023-03-14" @default.
• W4324017502 creator A5047655088 @default.
• W4324017502 creator A5051920874 @default.
• W4324017502 creator A5072754592 @default.
• W4324017502 creator A5087485806 @default.
• W4324017502 date "2023-03-13" @default.
• W4324017502 modified "2023-10-06" @default.
• W4324017502 title "A survey of elastase-producing bacteria and characteristics of the most potent producer, Priestia megaterium gasm32" @default.
• W4324017502 cites W1722214742 @default.
• W4324017502 cites W1874248752 @default.
• W4324017502 cites W1969731069 @default.
• W4324017502 cites W1974185456 @default.
• W4324017502 cites W1975273371 @default.
• W4324017502 cites W1978330686 @default.
• W4324017502 cites W1985090646 @default.
• W4324017502 cites W1987320941 @default.
• W4324017502 cites W2001966182 @default.
• W4324017502 cites W2006807779 @default.
• W4324017502 cites W2009852809 @default.
• W4324017502 cites W2015656398 @default.
• W4324017502 cites W2018289835 @default.
• W4324017502 cites W2038187462 @default.
• W4324017502 cites W2055455181 @default.
• W4324017502 cites W2059636296 @default.
• W4324017502 cites W2072373029 @default.
• W4324017502 cites W2072836683 @default.
• W4324017502 cites W2076168807 @default.
• W4324017502 cites W2077175699 @default.
• W4324017502 cites W2078323831 @default.
• W4324017502 cites W2080912121 @default.
• W4324017502 cites W2089349513 @default.
• W4324017502 cites W2095545972 @default.
• W4324017502 cites W2100837269 @default.
• W4324017502 cites W2145918400 @default.
• W4324017502 cites W2150816166 @default.
• W4324017502 cites W2154401636 @default.
• W4324017502 cites W2160862080 @default.
• W4324017502 cites W2163998847 @default.
• W4324017502 cites W2413850493 @default.
• W4324017502 cites W2558941103 @default.
• W4324017502 cites W2763465043 @default.
• W4324017502 cites W2801801636 @default.
• W4324017502 cites W2802056344 @default.
• W4324017502 cites W2809214793 @default.
• W4324017502 cites W2913043040 @default.
• W4324017502 cites W2949817856 @default.
• W4324017502 cites W2991502597 @default.
• W4324017502 cites W3112234213 @default.
• W4324017502 cites W3155557995 @default.
• W4324017502 cites W4211253901 @default.
• W4324017502 cites W4239845879 @default.
• W4324017502 cites W1993014359 @default.
• W4324017502 doi "https://doi.org/10.1371/journal.pone.0282963" @default.
• W4324017502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36913358" @default.
• W4324017502 hasPublicationYear "2023" @default.
• W4324017502 type Work @default.
• W4324017502 citedByCount "2" @default.
• W4324017502 countsByYear W43240175022023 @default.
• W4324017502 crossrefType "journal-article" @default.
• W4324017502 hasAuthorship W4324017502A5047655088 @default.
• W4324017502 hasAuthorship W4324017502A5051920874 @default.
• W4324017502 hasAuthorship W4324017502A5072754592 @default.
• W4324017502 hasAuthorship W4324017502A5087485806 @default.
• W4324017502 hasBestOaLocation W43240175021 @default.
• W4324017502 hasConcept C163994747 @default.
• W4324017502 hasConcept C181199279 @default.
• W4324017502 hasConcept C185592680 @default.
• W4324017502 hasConcept C2776326425 @default.
• W4324017502 hasConcept C2779869378 @default.
• W4324017502 hasConcept C2780989563 @default.
• W4324017502 hasConcept C2781044401 @default.
• W4324017502 hasConcept C43617362 @default.
• W4324017502 hasConcept C523546767 @default.
• W4324017502 hasConcept C54355233 @default.
• W4324017502 hasConcept C55493867 @default.
• W4324017502 hasConcept C86803240 @default.
• W4324017502 hasConcept C89423630 @default.
• W4324017502 hasConceptScore W4324017502C163994747 @default.
• W4324017502 hasConceptScore W4324017502C181199279 @default.
• W4324017502 hasConceptScore W4324017502C185592680 @default.
• W4324017502 hasConceptScore W4324017502C2776326425 @default.
• W4324017502 hasConceptScore W4324017502C2779869378 @default.
• W4324017502 hasConceptScore W4324017502C2780989563 @default.
• W4324017502 hasConceptScore W4324017502C2781044401 @default.
• W4324017502 hasConceptScore W4324017502C43617362 @default.
• W4324017502 hasConceptScore W4324017502C523546767 @default.
• W4324017502 hasConceptScore W4324017502C54355233 @default.
• W4324017502 hasConceptScore W4324017502C55493867 @default.
• W4324017502 hasConceptScore W4324017502C86803240 @default.
• W4324017502 hasConceptScore W4324017502C89423630 @default.
• W4324017502 hasIssue "3" @default.
• W4324017502 hasLocation W43240175021 @default.
• W4324017502 hasLocation W43240175022 @default.
• W4324017502 hasLocation W43240175023 @default.
• W4324017502 hasOpenAccess W4324017502 @default.
• W4324017502 hasPrimaryLocation W43240175021 @default.
• W4324017502 hasRelatedWork W1982544494 @default.

• next