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• W4324018032 abstract "Abstract Introduction This study evaluated the feasibility of the Sysmex XN‐3000 automated hematology analyzer for the assessment of total nucleated cells (TNC) and bone marrow (BM) cell density in routine bone marrow aspiration (BMA) samples. Methods A total of 54 BMA samples from 39 hematological patients were evaluated. The number of megakaryocytes was calculated by a specific gating algorithm using the body fluid mode of the WBC differential (WDF) channel. Lipid contents were calculated through a newly developed algorithm utilizing the WDF channel. The ratio of lipid particles over TNCs by the WNR channel was compared with the BM cellularity assessed by the BM biopsy. The myeloid/erythroid ( M / E ) ratio was calculated by measuring the number of myeloid cells in the WDF channel and the number of nucleated red blood cells (NRBCs) in the WNR channel. Results XN‐3000 counts and microscopic results showed a linear correlation in TNC ( R 2 = .98, p < .001), megakaryocytes ( R 2 = .59, p = .002), NRBC ( R 2 = .84, p < .001), and M / E ratio ( R 2 = .59, p < .001). There were significant differences in the lipid/TNC ratios of hypercellular, normocellular, and hypocellular BMs measured by XN‐3000 ( p < .001). Receiver‐operating characteristic analysis detected cut‐off values of the lipid/TNC ratio of >0.4054 for hypoplasia and <0.157 for hyperplasia. The sensitivity and specificity for hypoplasia were 100% and 88%, and for hyperplasia were 89% and 86%, respectively. Conclusion XN‐3000 provides a quantitative assessment of BM cellularity, supporting the qualitative assessment by myelogram and BM biopsy." @default.
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• W4324018032 date "2023-03-12" @default.
• W4324018032 modified "2023-10-14" @default.
• W4324018032 title "Evaluation of bone marrow aspirates using the automated hematology analyzer Sysmex <scp>XN</scp>‐3000" @default.
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• W4324018032 doi "https://doi.org/10.1111/ijlh.14058" @default.
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