FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4324055458> ?p ?o ?g. }

• W4324055458 endingPage "706" @default.
• W4324055458 startingPage "706" @default.
• W4324055458 abstract "Intracellular hydrogen peroxide (H2O2) levels can oscillate from low, physiological concentrations, to intermediate, signaling ones, and can participate in toxic reactions when overcoming certain thresholds. Fluorescent protein-based reporters to measure intracellular H2O2 have been developed in recent decades. In particular, the redox-sensitive green fluorescent protein (roGFP)-based proteins fused to peroxiredoxins are among the most sensitive H2O2 biosensors. Using fission yeast as a model system, we recently demonstrated that the gradient of extracellular-to-intracellular peroxides through the plasma membrane is around 300:1, and that the concentration of physiological H2O2 is in the low nanomolar range. Here, we have expressed the very sensitive probe roGFP2-Tpx1.C169S in two other model systems, budding yeast and human Jurkat cells. As in fission yeast, the biosensor is ~40–50% oxidized in these cell types, suggesting similar peroxide steady-state levels. Furthermore, probe oxidation upon the addition of extracellular peroxides is also quantitatively similar, suggesting comparable plasma membrane H2O2 gradients. Finally, as a proof of concept, we have applied different concentrations of zinc to all three model systems and have detected probe oxidation, demonstrating that an excess of this metal can cause fluctuations of peroxides, which are moderate in yeasts and severe in mammalian cells. We conclude that the principles governing H2O2 fluxes are very similar in different model organisms." @default.
• W4324055458 created "2023-03-14" @default.
• W4324055458 creator A5008368828 @default.
• W4324055458 creator A5036724867 @default.
• W4324055458 creator A5044391775 @default.
• W4324055458 creator A5044576329 @default.
• W4324055458 creator A5068666021 @default.
• W4324055458 creator A5077470696 @default.
• W4324055458 date "2023-03-13" @default.
• W4324055458 modified "2023-09-26" @default.
• W4324055458 title "Expression of the H2O2 Biosensor roGFP-Tpx1.C169S in Fission and Budding Yeasts and Jurkat Cells to Compare Intracellular H2O2 Levels, Transmembrane Gradients, and Response to Metals" @default.
• W4324055458 cites W1985962374 @default.
• W4324055458 cites W1990265320 @default.
• W4324055458 cites W2002132602 @default.
• W4324055458 cites W2019903373 @default.
• W4324055458 cites W2028053818 @default.
• W4324055458 cites W2036760275 @default.
• W4324055458 cites W2038931212 @default.
• W4324055458 cites W2043831708 @default.
• W4324055458 cites W2066739566 @default.
• W4324055458 cites W2069271483 @default.
• W4324055458 cites W2070085673 @default.
• W4324055458 cites W2074643442 @default.
• W4324055458 cites W2088053630 @default.
• W4324055458 cites W2088161712 @default.
• W4324055458 cites W2090055520 @default.
• W4324055458 cites W2091375521 @default.
• W4324055458 cites W2103195551 @default.
• W4324055458 cites W2112645157 @default.
• W4324055458 cites W2115273208 @default.
• W4324055458 cites W2119942926 @default.
• W4324055458 cites W2139923289 @default.
• W4324055458 cites W2145624692 @default.
• W4324055458 cites W2194986544 @default.
• W4324055458 cites W2273745031 @default.
• W4324055458 cites W2342179630 @default.
• W4324055458 cites W2613209849 @default.
• W4324055458 cites W2622794576 @default.
• W4324055458 cites W2805762229 @default.
• W4324055458 cites W2895930884 @default.
• W4324055458 cites W2904575974 @default.
• W4324055458 cites W2937511191 @default.
• W4324055458 cites W2941338208 @default.
• W4324055458 cites W2977278662 @default.
• W4324055458 cites W2996354046 @default.
• W4324055458 cites W3009127249 @default.
• W4324055458 cites W3009623318 @default.
• W4324055458 cites W3011833671 @default.
• W4324055458 cites W3013352992 @default.
• W4324055458 cites W3143347955 @default.
• W4324055458 cites W3158224783 @default.
• W4324055458 cites W3175484948 @default.
• W4324055458 cites W3196953889 @default.
• W4324055458 cites W3208161795 @default.
• W4324055458 cites W3210875053 @default.
• W4324055458 cites W4221006855 @default.
• W4324055458 doi "https://doi.org/10.3390/antiox12030706" @default.
• W4324055458 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36978953" @default.
• W4324055458 hasPublicationYear "2023" @default.
• W4324055458 type Work @default.
• W4324055458 citedByCount "0" @default.
• W4324055458 crossrefType "journal-article" @default.
• W4324055458 hasAuthorship W4324055458A5008368828 @default.
• W4324055458 hasAuthorship W4324055458A5036724867 @default.
• W4324055458 hasAuthorship W4324055458A5044391775 @default.
• W4324055458 hasAuthorship W4324055458A5044576329 @default.
• W4324055458 hasAuthorship W4324055458A5068666021 @default.
• W4324055458 hasAuthorship W4324055458A5077470696 @default.
• W4324055458 hasBestOaLocation W43240554581 @default.
• W4324055458 hasConcept C12554922 @default.
• W4324055458 hasConcept C160756335 @default.
• W4324055458 hasConcept C179464577 @default.
• W4324055458 hasConcept C185592680 @default.
• W4324055458 hasConcept C203014093 @default.
• W4324055458 hasConcept C2776090121 @default.
• W4324055458 hasConcept C2779222958 @default.
• W4324055458 hasConcept C28406088 @default.
• W4324055458 hasConcept C533411734 @default.
• W4324055458 hasConcept C55493867 @default.
• W4324055458 hasConcept C79879829 @default.
• W4324055458 hasConcept C86803240 @default.
• W4324055458 hasConcept C8891405 @default.
• W4324055458 hasConcept C95444343 @default.
• W4324055458 hasConceptScore W4324055458C12554922 @default.
• W4324055458 hasConceptScore W4324055458C160756335 @default.
• W4324055458 hasConceptScore W4324055458C179464577 @default.
• W4324055458 hasConceptScore W4324055458C185592680 @default.
• W4324055458 hasConceptScore W4324055458C203014093 @default.
• W4324055458 hasConceptScore W4324055458C2776090121 @default.
• W4324055458 hasConceptScore W4324055458C2779222958 @default.
• W4324055458 hasConceptScore W4324055458C28406088 @default.
• W4324055458 hasConceptScore W4324055458C533411734 @default.
• W4324055458 hasConceptScore W4324055458C55493867 @default.
• W4324055458 hasConceptScore W4324055458C79879829 @default.
• W4324055458 hasConceptScore W4324055458C86803240 @default.
• W4324055458 hasConceptScore W4324055458C8891405 @default.
• W4324055458 hasConceptScore W4324055458C95444343 @default.
• W4324055458 hasIssue "3" @default.

• next