Matches in SemOpenAlex for { <https://semopenalex.org/work/W4324058001> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W4324058001 endingPage "647" @default.
- W4324058001 startingPage "647" @default.
- W4324058001 abstract "Non-small cell lung cancer (NSCLC) is a major cause of cancer-related deaths around the world. Targeting the sensitized epidermal growth factor receptor (EGFR) caused by gene mutation through the tyrosine kinase inhibitor is an effective therapeutic strategy for NSCLC. Hence, the individualized therapeutic strategy has highlighted the demand for a simple, fast, and intelligent strategy for the genetic decoding of EGFR to cater to the popularization of precision medicine. In this research, a one-pot assay for EGFR identification is established by combining a loop-mediated isothermal amplification and amplification refractory mutation system. By optimizing the component and condition of the nucleic acid amplification system, a sensitive and specific distinguishability is achieved for tracing target variant (60 copies, 0.1%) identification under a strong interferential background within 40 min. Moreover, complex operation and time-consuming data processing, as well as the aerosol contamination, are avoided owing to the whole process for intelligent genetic decoding being performed in a sealed tube. As a demonstration, L858R, the primary point mutation for the sensitization of EGFR, has been accurately decoded using this assay with highly heterogeneous cancerous tissue. In addition, this method can be easily extended for other genetic information decoding using a tailor-made primer set. Thus, we propose that this straightforward strategy may serve as a promising tool for NSCLC diagnosis in clinical practice." @default.
- W4324058001 created "2023-03-14" @default.
- W4324058001 creator A5014463358 @default.
- W4324058001 creator A5018222948 @default.
- W4324058001 creator A5031568192 @default.
- W4324058001 date "2023-03-12" @default.
- W4324058001 modified "2023-10-18" @default.
- W4324058001 title "Intelligent Genetic Decoding System Based on Nucleic Acid Isothermal Amplification for Non-Small Cell Lung Cancer Diagnosis" @default.
- W4324058001 cites W1967394241 @default.
- W4324058001 cites W2007528734 @default.
- W4324058001 cites W2043696829 @default.
- W4324058001 cites W2048661839 @default.
- W4324058001 cites W2064063117 @default.
- W4324058001 cites W2105275554 @default.
- W4324058001 cites W2139236349 @default.
- W4324058001 cites W2171139285 @default.
- W4324058001 cites W2234519782 @default.
- W4324058001 cites W2268740575 @default.
- W4324058001 cites W2302518824 @default.
- W4324058001 cites W2327437619 @default.
- W4324058001 cites W2905253584 @default.
- W4324058001 cites W2976516489 @default.
- W4324058001 cites W2991555561 @default.
- W4324058001 cites W2993503316 @default.
- W4324058001 cites W3000719793 @default.
- W4324058001 cites W3009906937 @default.
- W4324058001 cites W3122039575 @default.
- W4324058001 cites W3127053396 @default.
- W4324058001 cites W3128646645 @default.
- W4324058001 cites W4243265752 @default.
- W4324058001 cites W4384455562 @default.
- W4324058001 doi "https://doi.org/10.3390/mi14030647" @default.
- W4324058001 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36985054" @default.
- W4324058001 hasPublicationYear "2023" @default.
- W4324058001 type Work @default.
- W4324058001 citedByCount "0" @default.
- W4324058001 crossrefType "journal-article" @default.
- W4324058001 hasAuthorship W4324058001A5014463358 @default.
- W4324058001 hasAuthorship W4324058001A5018222948 @default.
- W4324058001 hasAuthorship W4324058001A5031568192 @default.
- W4324058001 hasBestOaLocation W43240580011 @default.
- W4324058001 hasConcept C104317684 @default.
- W4324058001 hasConcept C121608353 @default.
- W4324058001 hasConcept C176944494 @default.
- W4324058001 hasConcept C24107716 @default.
- W4324058001 hasConcept C2779438470 @default.
- W4324058001 hasConcept C41008148 @default.
- W4324058001 hasConcept C501734568 @default.
- W4324058001 hasConcept C502942594 @default.
- W4324058001 hasConcept C54355233 @default.
- W4324058001 hasConcept C552990157 @default.
- W4324058001 hasConcept C60635243 @default.
- W4324058001 hasConcept C86803240 @default.
- W4324058001 hasConceptScore W4324058001C104317684 @default.
- W4324058001 hasConceptScore W4324058001C121608353 @default.
- W4324058001 hasConceptScore W4324058001C176944494 @default.
- W4324058001 hasConceptScore W4324058001C24107716 @default.
- W4324058001 hasConceptScore W4324058001C2779438470 @default.
- W4324058001 hasConceptScore W4324058001C41008148 @default.
- W4324058001 hasConceptScore W4324058001C501734568 @default.
- W4324058001 hasConceptScore W4324058001C502942594 @default.
- W4324058001 hasConceptScore W4324058001C54355233 @default.
- W4324058001 hasConceptScore W4324058001C552990157 @default.
- W4324058001 hasConceptScore W4324058001C60635243 @default.
- W4324058001 hasConceptScore W4324058001C86803240 @default.
- W4324058001 hasFunder F4320335777 @default.
- W4324058001 hasIssue "3" @default.
- W4324058001 hasLocation W43240580011 @default.
- W4324058001 hasLocation W43240580012 @default.
- W4324058001 hasLocation W43240580013 @default.
- W4324058001 hasOpenAccess W4324058001 @default.
- W4324058001 hasPrimaryLocation W43240580011 @default.
- W4324058001 hasRelatedWork W1480986388 @default.
- W4324058001 hasRelatedWork W1597038121 @default.
- W4324058001 hasRelatedWork W1993884661 @default.
- W4324058001 hasRelatedWork W2006505028 @default.
- W4324058001 hasRelatedWork W2047961713 @default.
- W4324058001 hasRelatedWork W2060452880 @default.
- W4324058001 hasRelatedWork W3122039575 @default.
- W4324058001 hasRelatedWork W3161671396 @default.
- W4324058001 hasRelatedWork W4318678602 @default.
- W4324058001 hasRelatedWork W2173258789 @default.
- W4324058001 hasVolume "14" @default.
- W4324058001 isParatext "false" @default.
- W4324058001 isRetracted "false" @default.
- W4324058001 workType "article" @default.