Matches in SemOpenAlex for { <https://semopenalex.org/work/W4324087295> ?p ?o ?g. }
- W4324087295 abstract "Penicillin G acylase plays an important role in the biocatalytic process of semi-synthetic penicillin. In order to overcome the disadvantages of free enzymes and improve the catalytic performance of enzymes, it is a new method to immobilize enzymes on carrier materials. And magnetic materials have the characteristics of easy separation. In the present study, the Magnetic Ni0.3Mg0.4Zn0.3Fe2O4 nanoparticles were successfully prepared by a rapid-combustion method and calcined at 400°C for 2 h. The surface of the nanoparticles was modified with sodium silicate hydrate, and the PGA was covalently bound to the carrier particles through the cross-linking of glutaraldehyde. The results showed that the activity of immobilized PGA reached 7121.00 U/g. The optimum pH for immobilized PGA was 8 and the optimum temperature was 45°C, the immobilized PGA exhibited higher stability against changes in pH and temperature. The Michaelis-Menten constant (Km) values of the free and immobilized PGA were 0.00387 and 0.0101 mol/L and the maximum rate (Vmax) values were 0.387 and 0.129 μmol/min. Besides, the immobilized PGA revealed excellent cycling performance. The immobilization strategy presented PGA had the advantages of reuse, good stability, cost saving and had considerable practical significance for the commercial application of PGA." @default.
- W4324087295 created "2023-03-14" @default.
- W4324087295 creator A5017116708 @default.
- W4324087295 creator A5020376217 @default.
- W4324087295 creator A5021561750 @default.
- W4324087295 creator A5067796934 @default.
- W4324087295 creator A5075877965 @default.
- W4324087295 creator A5090068965 @default.
- W4324087295 date "2023-03-13" @default.
- W4324087295 modified "2023-09-28" @default.
- W4324087295 title "Immobilization and property of penicillin G acylase on amino functionalized magnetic Ni0.3Mg0.4Zn0.3Fe2O4 nanoparticles prepared via the rapid combustion process" @default.
- W4324087295 cites W1768451682 @default.
- W4324087295 cites W1988270240 @default.
- W4324087295 cites W1993832123 @default.
- W4324087295 cites W2003892115 @default.
- W4324087295 cites W2005476581 @default.
- W4324087295 cites W2012484827 @default.
- W4324087295 cites W2044430464 @default.
- W4324087295 cites W2073016734 @default.
- W4324087295 cites W2074139440 @default.
- W4324087295 cites W2094738631 @default.
- W4324087295 cites W2158261322 @default.
- W4324087295 cites W2417467445 @default.
- W4324087295 cites W2520985447 @default.
- W4324087295 cites W2765560714 @default.
- W4324087295 cites W2792956883 @default.
- W4324087295 cites W2801250674 @default.
- W4324087295 cites W2894969919 @default.
- W4324087295 cites W2902461467 @default.
- W4324087295 cites W2931537435 @default.
- W4324087295 cites W2936182651 @default.
- W4324087295 cites W2945698650 @default.
- W4324087295 cites W2987860459 @default.
- W4324087295 cites W3044431631 @default.
- W4324087295 cites W3046849762 @default.
- W4324087295 cites W3105724871 @default.
- W4324087295 cites W3129036857 @default.
- W4324087295 cites W3129804030 @default.
- W4324087295 cites W3212971464 @default.
- W4324087295 cites W4205179774 @default.
- W4324087295 cites W4206763398 @default.
- W4324087295 cites W4210302899 @default.
- W4324087295 cites W4210359703 @default.
- W4324087295 cites W4220777354 @default.
- W4324087295 cites W4224879756 @default.
- W4324087295 cites W4281492533 @default.
- W4324087295 cites W4284965515 @default.
- W4324087295 cites W4285400597 @default.
- W4324087295 cites W4285801616 @default.
- W4324087295 cites W4286425576 @default.
- W4324087295 cites W4286565940 @default.
- W4324087295 cites W4289259949 @default.
- W4324087295 cites W4289546912 @default.
- W4324087295 cites W4291134821 @default.
- W4324087295 cites W4292866427 @default.
- W4324087295 cites W4297995761 @default.
- W4324087295 doi "https://doi.org/10.3389/fbioe.2023.1108820" @default.
- W4324087295 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36994365" @default.
- W4324087295 hasPublicationYear "2023" @default.
- W4324087295 type Work @default.
- W4324087295 citedByCount "0" @default.
- W4324087295 crossrefType "journal-article" @default.
- W4324087295 hasAuthorship W4324087295A5017116708 @default.
- W4324087295 hasAuthorship W4324087295A5020376217 @default.
- W4324087295 hasAuthorship W4324087295A5021561750 @default.
- W4324087295 hasAuthorship W4324087295A5067796934 @default.
- W4324087295 hasAuthorship W4324087295A5075877965 @default.
- W4324087295 hasAuthorship W4324087295A5090068965 @default.
- W4324087295 hasBestOaLocation W43240872951 @default.
- W4324087295 hasConcept C127413603 @default.
- W4324087295 hasConcept C13965031 @default.
- W4324087295 hasConcept C155672457 @default.
- W4324087295 hasConcept C161790260 @default.
- W4324087295 hasConcept C171250308 @default.
- W4324087295 hasConcept C178790620 @default.
- W4324087295 hasConcept C180577832 @default.
- W4324087295 hasConcept C181199279 @default.
- W4324087295 hasConcept C185592680 @default.
- W4324087295 hasConcept C192562407 @default.
- W4324087295 hasConcept C2777451570 @default.
- W4324087295 hasConcept C2777529624 @default.
- W4324087295 hasConcept C33633552 @default.
- W4324087295 hasConcept C42360764 @default.
- W4324087295 hasConcept C43617362 @default.
- W4324087295 hasConcept C7082614 @default.
- W4324087295 hasConcept C82776694 @default.
- W4324087295 hasConcept C87023908 @default.
- W4324087295 hasConceptScore W4324087295C127413603 @default.
- W4324087295 hasConceptScore W4324087295C13965031 @default.
- W4324087295 hasConceptScore W4324087295C155672457 @default.
- W4324087295 hasConceptScore W4324087295C161790260 @default.
- W4324087295 hasConceptScore W4324087295C171250308 @default.
- W4324087295 hasConceptScore W4324087295C178790620 @default.
- W4324087295 hasConceptScore W4324087295C180577832 @default.
- W4324087295 hasConceptScore W4324087295C181199279 @default.
- W4324087295 hasConceptScore W4324087295C185592680 @default.
- W4324087295 hasConceptScore W4324087295C192562407 @default.
- W4324087295 hasConceptScore W4324087295C2777451570 @default.
- W4324087295 hasConceptScore W4324087295C2777529624 @default.
- W4324087295 hasConceptScore W4324087295C33633552 @default.