Matches in SemOpenAlex for { <https://semopenalex.org/work/W4324128731> ?p ?o ?g. }
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- W4324128731 abstract "The establishment of host-microbe interactions requires molecular communication between both partners, which may involve the mutual transfer of noncoding small RNAs. Previous evidence suggests that this is also true for powdery mildew disease in barley, which is caused by the fungal pathogen Blumeria hordei. However, previous studies lacked spatial resolution regarding the accumulation of small RNAs upon host infection by B. hordei. Here, we analysed site-specific small RNA repertoires in the context of the barley-B. hordei interaction. To this end, we dissected infected leaves into separate fractions representing different sites that are key to the pathogenic process: epiphytic fungal mycelium, infected plant epidermis, isolated haustoria, a vesicle-enriched fraction from infected epidermis, and extracellular vesicles. Unexpectedly, we discovered enrichment of specific 31-33-base 5'-terminal fragments of barley 5.8S ribosomal RNA in extracellular vesicles and infected epidermis, as well as particular B. hordei transfer RNA fragments in haustoria. We describe canonical small RNAs from both the plant host and the fungal pathogen that may confer cross-kingdom RNA interference activity. Interestingly, we found first evidence of phased small interfering RNAs in B. hordei, a feature usually attributed to plants, which may be associated with the posttranscriptional control of fungal coding genes, pseudogenes, and transposable elements. Our data suggest a key and possibly site-specific role for cross-kingdom RNA interference and noncoding RNA fragments in the host-pathogen communication between B. hordei and its host barley." @default.
- W4324128731 created "2023-03-15" @default.
- W4324128731 creator A5003945330 @default.
- W4324128731 creator A5019905622 @default.
- W4324128731 creator A5036048833 @default.
- W4324128731 creator A5076127425 @default.
- W4324128731 creator A5091430171 @default.
- W4324128731 date "2023-03-14" @default.
- W4324128731 modified "2023-10-18" @default.
- W4324128731 title "Site‐specific analysis reveals candidate cross‐kingdom small RNAs, tRNA and rRNA fragments, and signs of fungal RNA phasing in the barley–powdery mildew interaction" @default.
- W4324128731 cites W1639310471 @default.
- W4324128731 cites W1647075334 @default.
- W4324128731 cites W1952710215 @default.
- W4324128731 cites W1966327575 @default.
- W4324128731 cites W1967276739 @default.
- W4324128731 cites W1969353942 @default.
- W4324128731 cites W1973012451 @default.
- W4324128731 cites W1978271061 @default.
- W4324128731 cites W1979730944 @default.
- W4324128731 cites W1979979849 @default.
- W4324128731 cites W1988501806 @default.
- W4324128731 cites W1993587057 @default.
- W4324128731 cites W1996315076 @default.
- W4324128731 cites W2017014505 @default.
- W4324128731 cites W2018221572 @default.
- W4324128731 cites W2026566929 @default.
- W4324128731 cites W2036897871 @default.
- W4324128731 cites W2048586937 @default.
- W4324128731 cites W2054077259 @default.
- W4324128731 cites W2063159592 @default.
- W4324128731 cites W2089979890 @default.
- W4324128731 cites W2102619694 @default.
- W4324128731 cites W2108234281 @default.
- W4324128731 cites W2109140791 @default.
- W4324128731 cites W2111092051 @default.
- W4324128731 cites W2114104545 @default.
- W4324128731 cites W2123614057 @default.
- W4324128731 cites W2127446611 @default.
- W4324128731 cites W2131271579 @default.
- W4324128731 cites W2137526110 @default.
- W4324128731 cites W2138207763 @default.
- W4324128731 cites W2140166150 @default.
- W4324128731 cites W2144177541 @default.
- W4324128731 cites W2148419405 @default.
- W4324128731 cites W2151435150 @default.
- W4324128731 cites W2154241726 @default.
- W4324128731 cites W2161159960 @default.
- W4324128731 cites W2161479301 @default.
- W4324128731 cites W2163370673 @default.
- W4324128731 cites W2165845089 @default.
- W4324128731 cites W2170609747 @default.
- W4324128731 cites W2179438025 @default.
- W4324128731 cites W2341732483 @default.
- W4324128731 cites W2472783066 @default.
- W4324128731 cites W2521150917 @default.
- W4324128731 cites W2523875929 @default.
- W4324128731 cites W2524983888 @default.
- W4324128731 cites W2529838505 @default.
- W4324128731 cites W2553193414 @default.
- W4324128731 cites W2555190540 @default.
- W4324128731 cites W2559640639 @default.
- W4324128731 cites W2561592872 @default.
- W4324128731 cites W2584254450 @default.
- W4324128731 cites W2586349554 @default.
- W4324128731 cites W2609489517 @default.
- W4324128731 cites W2610080502 @default.
- W4324128731 cites W2729511311 @default.
- W4324128731 cites W2765185461 @default.
- W4324128731 cites W2787958654 @default.
- W4324128731 cites W2803645366 @default.
- W4324128731 cites W2887163353 @default.
- W4324128731 cites W2887353955 @default.
- W4324128731 cites W2889945294 @default.
- W4324128731 cites W2892306122 @default.
- W4324128731 cites W2904815792 @default.
- W4324128731 cites W2905137290 @default.
- W4324128731 cites W2907183438 @default.
- W4324128731 cites W2916031961 @default.
- W4324128731 cites W2940509414 @default.
- W4324128731 cites W2949076534 @default.
- W4324128731 cites W2950847039 @default.
- W4324128731 cites W2950954328 @default.
- W4324128731 cites W2962676603 @default.
- W4324128731 cites W2963067047 @default.
- W4324128731 cites W2963725094 @default.
- W4324128731 cites W2964623003 @default.
- W4324128731 cites W2973650226 @default.
- W4324128731 cites W2983828425 @default.
- W4324128731 cites W2991519939 @default.
- W4324128731 cites W2996828802 @default.
- W4324128731 cites W2997022986 @default.
- W4324128731 cites W3004267120 @default.
- W4324128731 cites W3023173231 @default.
- W4324128731 cites W3027980964 @default.
- W4324128731 cites W3056583907 @default.
- W4324128731 cites W3129117484 @default.
- W4324128731 cites W3137889167 @default.
- W4324128731 cites W3159852584 @default.