FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4324129078> ?p ?o ?g. }

• W4324129078 abstract "CgtA is an essential bacterial GTPase consisting of a highly conserved N-terminal Spo0B-associated GTP-binding protein (Obg) domain, a central GTPase domain, and a variable C-terminal domain (CTD). This study reports global changes in the proteome and transcriptome of wild-type (Wt) versus full-length CgtA-depleted Vibrio cholerae in minimal media. Comparative transcriptome sequencing (RNA-Seq), followed by comparative proteomic analyses, revealed that the knockdown of cgtA significantly altered expressions of 311 proteins involved in diverse cellular activities, many of which are associated with the survival of V. cholerae. Various intracellular functional roles of CgtA in growth, viability, motility, morphology, and persister phenotypes in V. cholerae are revealed based on subsequent confirmatory experiments. Furthermore, a more sustained mRNA expression pattern of cgtA in a minimal medium than in a rich medium was also observed for Wt V. cholerae, where the highest level of mRNA expression of cgtA was observed during the logarithmic growth phase. Thereby, we propose that minimal medium-associated reduced growth rate coupled with cgtA depletion aggravates the intracellular stress in V. cholerae, interrupting vital cellular processes. The functional role of the CTD in V. cholerae is not fully understood. Hence, to specifically investigate the intracellular role of the 57-amino-acid-long CTD of CgtAVC, the CTD-only portion of CgtA was deleted. Subsequent proteomics studies revealed an altered expression of 240 proteins in the CgtA(ΔCTD) mutant, having major overlap with the full-length cgtA-deleted condition. Overall, our study reveals an alternative facet of the survival mechanism of V. cholerae during nutritional downshift as per the concomitant consequences of cgtA depletion. IMPORTANCE It is very important that we must find new drug target proteins from multidrug-resistant human-pathogenic organisms like V. cholerae. CgtA is among such potential candidates, and here, we are reporting about some newly identified cellular roles of it that are important for the survival of V. cholerae. Briefly, we knocked down the full-length cgtA gene, as well as did a partial deletion of this gene from the V. cholerae genome followed by RNA-Seq and proteomics studies. Results from our study revealed up- and downregulation of several known and unknown genes and proteins as the effect of the cgtA knockdown experiment. Also, we have presented some interesting observations that are linked with cgtA for growth, viability, motility, morphology, and persister phenotypes in V. cholerae. Our study enhances the importance of CgtA and paves the way for further exploration based on our provided data." @default.
• W4324129078 created "2023-03-15" @default.
• W4324129078 creator A5018540639 @default.
• W4324129078 creator A5034666842 @default.
• W4324129078 creator A5042439991 @default.
• W4324129078 date "2023-04-13" @default.
• W4324129078 modified "2023-10-18" @default.
• W4324129078 title "Knockdown Experiment Reveals an Essential GTPase CgtA’s Involvement in Growth, Viability, Motility, Morphology, and Persister Phenotypes in Vibrio cholerae" @default.
• W4324129078 cites W1554952163 @default.
• W4324129078 cites W1950350632 @default.
• W4324129078 cites W1980210900 @default.
• W4324129078 cites W2009900253 @default.
• W4324129078 cites W2019365934 @default.
• W4324129078 cites W2026437584 @default.
• W4324129078 cites W2027358876 @default.
• W4324129078 cites W2028999572 @default.
• W4324129078 cites W2046901711 @default.
• W4324129078 cites W2068375963 @default.
• W4324129078 cites W2073573730 @default.
• W4324129078 cites W2075532221 @default.
• W4324129078 cites W2076783814 @default.
• W4324129078 cites W2094513524 @default.
• W4324129078 cites W2100821807 @default.
• W4324129078 cites W2102789355 @default.
• W4324129078 cites W2109947154 @default.
• W4324129078 cites W2111329583 @default.
• W4324129078 cites W2122543489 @default.
• W4324129078 cites W2141378551 @default.
• W4324129078 cites W2148417715 @default.
• W4324129078 cites W2153800679 @default.
• W4324129078 cites W2294572471 @default.
• W4324129078 cites W2345945462 @default.
• W4324129078 cites W2557306438 @default.
• W4324129078 cites W2591182649 @default.
• W4324129078 cites W2606713195 @default.
• W4324129078 cites W2740650521 @default.
• W4324129078 cites W2791125030 @default.
• W4324129078 cites W2803711611 @default.
• W4324129078 cites W2808258704 @default.
• W4324129078 cites W2890219238 @default.
• W4324129078 cites W2921268587 @default.
• W4324129078 cites W2967400239 @default.
• W4324129078 cites W3019439774 @default.
• W4324129078 cites W3047350338 @default.
• W4324129078 cites W3094868867 @default.
• W4324129078 cites W3200345170 @default.
• W4324129078 cites W3208691473 @default.
• W4324129078 doi "https://doi.org/10.1128/spectrum.03181-22" @default.
• W4324129078 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36916969" @default.
• W4324129078 hasPublicationYear "2023" @default.
• W4324129078 type Work @default.
• W4324129078 citedByCount "0" @default.
• W4324129078 crossrefType "journal-article" @default.
• W4324129078 hasAuthorship W4324129078A5018540639 @default.
• W4324129078 hasAuthorship W4324129078A5034666842 @default.
• W4324129078 hasAuthorship W4324129078A5042439991 @default.
• W4324129078 hasBestOaLocation W43241290781 @default.
• W4324129078 hasConcept C104317684 @default.
• W4324129078 hasConcept C104397665 @default.
• W4324129078 hasConcept C127716648 @default.
• W4324129078 hasConcept C150194340 @default.
• W4324129078 hasConcept C162317418 @default.
• W4324129078 hasConcept C173396325 @default.
• W4324129078 hasConcept C207332259 @default.
• W4324129078 hasConcept C2778643871 @default.
• W4324129078 hasConcept C2780298669 @default.
• W4324129078 hasConcept C523546767 @default.
• W4324129078 hasConcept C54355233 @default.
• W4324129078 hasConcept C58207958 @default.
• W4324129078 hasConcept C62478195 @default.
• W4324129078 hasConcept C79879829 @default.
• W4324129078 hasConcept C86803240 @default.
• W4324129078 hasConcept C95444343 @default.
• W4324129078 hasConceptScore W4324129078C104317684 @default.
• W4324129078 hasConceptScore W4324129078C104397665 @default.
• W4324129078 hasConceptScore W4324129078C127716648 @default.
• W4324129078 hasConceptScore W4324129078C150194340 @default.
• W4324129078 hasConceptScore W4324129078C162317418 @default.
• W4324129078 hasConceptScore W4324129078C173396325 @default.
• W4324129078 hasConceptScore W4324129078C207332259 @default.
• W4324129078 hasConceptScore W4324129078C2778643871 @default.
• W4324129078 hasConceptScore W4324129078C2780298669 @default.
• W4324129078 hasConceptScore W4324129078C523546767 @default.
• W4324129078 hasConceptScore W4324129078C54355233 @default.
• W4324129078 hasConceptScore W4324129078C58207958 @default.
• W4324129078 hasConceptScore W4324129078C62478195 @default.
• W4324129078 hasConceptScore W4324129078C79879829 @default.
• W4324129078 hasConceptScore W4324129078C86803240 @default.
• W4324129078 hasConceptScore W4324129078C95444343 @default.
• W4324129078 hasFunder F4320334771 @default.
• W4324129078 hasIssue "2" @default.
• W4324129078 hasLocation W43241290781 @default.
• W4324129078 hasLocation W43241290782 @default.
• W4324129078 hasLocation W43241290783 @default.
• W4324129078 hasOpenAccess W4324129078 @default.
• W4324129078 hasPrimaryLocation W43241290781 @default.
• W4324129078 hasRelatedWork W1152271471 @default.
• W4324129078 hasRelatedWork W2042678520 @default.
• W4324129078 hasRelatedWork W2080146774 @default.
• W4324129078 hasRelatedWork W2084295528 @default.

• next