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- W4324142004 abstract "Purpose: Isolation and cultivation of human articular chondrocytes (ACs) has been a widely used research technique for the study of the pathogenesis of osteoarthritis (OA), the screening OA drug candidates, and articular cartilage repair using autologous chondrocytes. Cartilage tissue engineering or autologous chondrocyte implantation (ACI) for cartilage repair requires large numbers of primary ACs for further expansions because repeated passages of cultivated ACs may result in cell “dedifferentiation” which describes the conversion of chondrocytes into fibroblast-like cells with the shift of extracellular matrix production from type II collagen to type I collagen. Since human autologous ACs used for cartilage repair are usually isolated from a non-weight bearing or non-lesion area of articular cartilage from the same joint with OA or focal cartilage lesions, it is paramount to optimize the methods for isolating and harvesting human primary chondrocytes from OA joints. The purpose of this study was to explore the best time point to obtain the maximum number of healthy ACs from a limited amount of articular cartilage in a non-lesion area of human OA joints." @default.
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- W4324142004 date "2023-03-01" @default.
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- W4324142004 title "Time-Dependent Cell Release Rate And Gene Expression Profile In Human Articular Chondrocyte Isolation" @default.
- W4324142004 doi "https://doi.org/10.1016/j.joca.2023.01.335" @default.
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