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• W4324143779 abstract "Summary Staphylococcus aureus frequently produces biofilm and causes human infections associated with the ingestion of contaminated food. Based on the acknowledgement that the antibiofilm agents for methicillin‐resistant S . aureus (MRSA) have not been developed yet, this study investigated the potentiality of the JM01 bacteriocin produced by Pediococcus acidilactici JM01 as an effective antibiofilm agent for MRSA. To investigate the antibiofilm activity of bacteriocin JM01 and its mechanism, antibacterial and anti‐adhesive property assays were performed against MRSA. The effect of JM01 bacteriocin on MRSA biofilm formation was examined by crystal violet staining, the 2,3‐bis‐[2‐methoxy‐4‐nitro‐5‐sulphophenyl]‐2H‐tetrazolium‐5‐carboxanilide reduction assay and scanning electron microscopy analysis. The inhibition effect of JM01 bacteriocin on MRSA growth was examined by spot plate assay and spectrophotometry. Confocal laser scanning microscopy and the bicinchoninic acid method were conducted to determine the adhesion of JM01 bacteriocin for preventing MRSA adhesion to the polystyrene surface. The results revealed that JM01 bacteriocin significantly reduced the biofilm formation of MRSA by inhibiting its growth and adhesion to a polystyrene surface. These results demonstrate that JM01 bacteriocin could be a potential candidate for the regulation of biofilm formation by MRSA." @default.
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• W4324143779 date "2023-03-26" @default.
• W4324143779 modified "2023-09-26" @default.
• W4324143779 title "Antibiofilm activity of crude bacteriocin JM01 produced by <i>Pediococcus acidilactici</i> against methicillin‐resistant <i>Staphylococcus aureus</i> (MRSA)" @default.
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• W4324143779 doi "https://doi.org/10.1111/ijfs.16407" @default.
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