Matches in SemOpenAlex for { <https://semopenalex.org/work/W4324149400> ?p ?o ?g. }
- W4324149400 endingPage "888" @default.
- W4324149400 startingPage "888" @default.
- W4324149400 abstract "Tracheal reconstruction represents a challenge when primary anastomosis is not feasible. Within this scenario, the study aim was to develop a new pig-derived decellularized trachea (DecellT) to be compared with the cryopreserved counterpart (CryoT) for a close predictive analysis. Tracheal segments underwent decellularization by a physical + enzymatic + chemical method (12 cycles); in parallel, cryopreserved samples were also prepared. Once decellularized (histology/DNA quantification), the two groups were characterized for Alpha-Gal epitopes/structural proteins (immunohistochemistry/histology/biochemical assays/second harmonic generation microscopy)/ultrastructure (Scanning Electron Microscopy (SEM))/mechanical behaviour. Cytotoxicity absence was assessed in vitro (extract-test assay/direct seeding, HM1SV40 cell line) while biocompatibility was verified in BALB/c mice, followed by histological/immunohistochemical analyses and SEM (14 days). Decellularization effectively removed Alpha-Gal epitopes; cartilage histoarchitecture was retained in both groups, showing chondrocytes only in the CryoT. Cryopreservation maintained few respiratory epithelium sparse cilia, not detectable in DecellT. Focusing on ECM, preserved structural/ultrastructural organization and collagen content were observed in the cartilage of both; conversely, the GAGs were significantly reduced in DecellT, as confirmed by mechanical study results. No cytotoxicity was highlighted by CryoT/DecellT in vitro, as they were also corroborated by a biocompatibility assay. Despite some limitations (cells presence/GAGs reduction), CryoT/DecellT are both appealing options, which warrant further investigation in comparative in vivo studies." @default.
- W4324149400 created "2023-03-15" @default.
- W4324149400 creator A5000666523 @default.
- W4324149400 creator A5010380702 @default.
- W4324149400 creator A5018896366 @default.
- W4324149400 creator A5019076220 @default.
- W4324149400 creator A5027463490 @default.
- W4324149400 creator A5027680474 @default.
- W4324149400 creator A5030053187 @default.
- W4324149400 creator A5030838370 @default.
- W4324149400 creator A5034239187 @default.
- W4324149400 creator A5040267840 @default.
- W4324149400 creator A5043808387 @default.
- W4324149400 creator A5044171254 @default.
- W4324149400 creator A5050377290 @default.
- W4324149400 creator A5054097399 @default.
- W4324149400 creator A5063956956 @default.
- W4324149400 creator A5070141661 @default.
- W4324149400 creator A5072679139 @default.
- W4324149400 creator A5086819522 @default.
- W4324149400 date "2023-03-13" @default.
- W4324149400 modified "2023-10-01" @default.
- W4324149400 title "Development and In Vitro/In Vivo Comparative Characterization of Cryopreserved and Decellularized Tracheal Grafts" @default.
- W4324149400 cites W131947895 @default.
- W4324149400 cites W1485298061 @default.
- W4324149400 cites W1509119336 @default.
- W4324149400 cites W1583945186 @default.
- W4324149400 cites W1849328966 @default.
- W4324149400 cites W1965083810 @default.
- W4324149400 cites W1965783971 @default.
- W4324149400 cites W1967543597 @default.
- W4324149400 cites W1968905776 @default.
- W4324149400 cites W1969393486 @default.
- W4324149400 cites W1970801820 @default.
- W4324149400 cites W1971661372 @default.
- W4324149400 cites W1977295503 @default.
- W4324149400 cites W1984133400 @default.
- W4324149400 cites W1986821124 @default.
- W4324149400 cites W1989928065 @default.
- W4324149400 cites W1993104226 @default.
- W4324149400 cites W1993269625 @default.
- W4324149400 cites W1993799451 @default.
- W4324149400 cites W1994257897 @default.
- W4324149400 cites W1994643050 @default.
- W4324149400 cites W1994649980 @default.
- W4324149400 cites W1998258100 @default.
- W4324149400 cites W1999492481 @default.
- W4324149400 cites W2000487674 @default.
- W4324149400 cites W2003270898 @default.
- W4324149400 cites W2011159943 @default.
- W4324149400 cites W2013001762 @default.
- W4324149400 cites W2015155184 @default.
- W4324149400 cites W2017466137 @default.
- W4324149400 cites W2020315602 @default.
- W4324149400 cites W2021029563 @default.
- W4324149400 cites W2022790312 @default.
- W4324149400 cites W2023186131 @default.
- W4324149400 cites W2024100316 @default.
- W4324149400 cites W2032622734 @default.
- W4324149400 cites W2032636386 @default.
- W4324149400 cites W2034746004 @default.
- W4324149400 cites W2045224926 @default.
- W4324149400 cites W2045974392 @default.
- W4324149400 cites W2054250836 @default.
- W4324149400 cites W2059486645 @default.
- W4324149400 cites W2060031017 @default.
- W4324149400 cites W2068363612 @default.
- W4324149400 cites W2073572965 @default.
- W4324149400 cites W2076603866 @default.
- W4324149400 cites W2082108397 @default.
- W4324149400 cites W2083631540 @default.
- W4324149400 cites W2083640507 @default.
- W4324149400 cites W2085289337 @default.
- W4324149400 cites W2086230834 @default.
- W4324149400 cites W2087521379 @default.
- W4324149400 cites W2094258709 @default.
- W4324149400 cites W2106584678 @default.
- W4324149400 cites W2112658126 @default.
- W4324149400 cites W2124221116 @default.
- W4324149400 cites W2125962661 @default.
- W4324149400 cites W2132795383 @default.
- W4324149400 cites W2140503670 @default.
- W4324149400 cites W2147277269 @default.
- W4324149400 cites W2149258342 @default.
- W4324149400 cites W2150126124 @default.
- W4324149400 cites W2166804282 @default.
- W4324149400 cites W2167279371 @default.
- W4324149400 cites W2168775089 @default.
- W4324149400 cites W2169258235 @default.
- W4324149400 cites W2170666164 @default.
- W4324149400 cites W2171850360 @default.
- W4324149400 cites W2252889477 @default.
- W4324149400 cites W2288817082 @default.
- W4324149400 cites W2319795507 @default.
- W4324149400 cites W2330270756 @default.
- W4324149400 cites W2337672429 @default.
- W4324149400 cites W2345284931 @default.
- W4324149400 cites W2409559862 @default.