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• W4324154057 abstract "Early detection of rare mutations through liquid biopsy can provide real-time information related to cancer diagnosis, prognosis, and treatment outcomes. Cell-free DNA samples used in liquid biopsies contain single-nucleotide variants (SNVs) with a variant allele frequency (VAF) of approximately ≤1%. Droplet digital polymerase chain reaction (ddPCR) is considered the gold standard of sequencing using liquid samples, generating amplicons from samples containing mutations with 0.001-0.005% VAF; however, it requires expensive equipment and time-consuming protocols. Therefore, various PCR methods for discriminating SNVs have been developed; nonetheless, non-specific amplification cannot be avoided even in the absence of mutations, which hampers the accurate diagnosis of SNVs. In this study, we introduce single-nucleotide variant on-off discrimination-PCR (Soo-PCR), a highly accurate and practical method that uses a 3'-end tailing primer for the on-off discrimination of low-abundance mutant-type targets, including SNVs. Soo-PCR minimizes the chance of incorrect judgments owing to its high discriminating power. Cancer markers, such as KRAS G12D, EGFR L858R, and EGFR T790M mutations, containing 0.1% VAF, were clearly detected in under 2 h with a high reliability comparable with that of ddPCR. This new method serves as a practical approach to accurately detect and evaluate low-abundance mutations in a user-friendly manner." @default.
• W4324154057 created "2023-03-15" @default.
• W4324154057 creator A5063610227 @default.
• W4324154057 creator A5066112386 @default.
• W4324154057 date "2023-03-13" @default.
• W4324154057 modified "2023-09-26" @default.
• W4324154057 title "Improving the Accuracy of Single-Nucleotide Variant Diagnosis Using On–Off Discriminating Primers" @default.
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• W4324154057 doi "https://doi.org/10.3390/bios13030380" @default.
• W4324154057 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36979592" @default.
• W4324154057 hasPublicationYear "2023" @default.
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