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- W4324332937 abstract "This 30-color full spectrum flow cytometry panel was developed and optimized for in-depth analysis T cells immunophenotype in tumor microenvironment and peripheral lymphoid organs. The panel presented here first identify the main cell subsets including myeloid cells, B cells, NKT cells, γδ T cells, CD4+ T cells and CD8+ T cells. For CD4+ T cells or CD8+ T cells, the panel includes markers for further characterization by including a selection of activation status(CD44, CD62L, CD69, Ki67, CD127, KLRG1 and CXCR3), costimulatory/co-inhibitory molecules (ICOS, OX-40, PD-1, LAG3, TIM-3, CTLA-4 and TIGIT), pro-inflammatory/anti-inflammatory cytokines (IFN-γ, TNF-α and IL-10) and cytotoxic molecules (Perforin, Granzymes B and CD107a). The panel has been tested on the tumor infiltrating T cells and corresponding spleen T cells in B16-F10 murine melanoma models." @default.
- W4324332937 created "2023-03-16" @default.
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- W4324332937 date "2023-05-01" @default.
- W4324332937 modified "2023-09-30" @default.
- W4324332937 title "30-color full spectrum flow cytometry panel for deep immunophenotyping of T cell subsets in murine tumor tissue" @default.
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- W4324332937 doi "https://doi.org/10.1016/j.jim.2023.113459" @default.
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