FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4324349380> ?p ?o ?g. }

• W4324349380 abstract "ABSTRACT The CLC-ec1 chloride/proton antiporter is a membrane embedded homodimer where subunits can dissociate and associate, but the thermodynamic driving forces favor the assembled form at biological densities. Yet, the physical reasons for this stability are confounding since binding occurs via the burial of hydrophobic protein interfaces yet the hydrophobic effect should not apply since there is little water within the membrane. To investigate this further, we quantified the thermodynamic changes associated with CLC dimerization in membranes by carrying out a van ′t Hoff analysis of the temperature dependency of the free energy of dimerization, ΔG° . To ensure that the reaction reached equilibrium under changing conditions, we utilized a Förster Resonance Energy Transfer based assay to report on the relaxation kinetics of subunit exchange as a function of temperature. These equilibration times were then applied to measure CLC-ec1 dimerization isotherms as a function of temperature using the single-molecule subunit-capture photobleaching analysis approach. The results demonstrate that the dimerization free energy of CLC in E. coli membranes exhibits a non-linear temperature dependency corresponding to a large, negative change in heat capacity, a signature of solvent ordering effects including the hydrophobic effect. Consolidating this with our previous molecular analyses suggests that the non-bilayer defect required to solvate the monomeric state is the molecular source of this large change in heat capacity and is a major and generalizable driving force for protein association in membranes." @default.
• W4324349380 created "2023-03-16" @default.
• W4324349380 creator A5006202510 @default.
• W4324349380 creator A5022192946 @default.
• W4324349380 creator A5026527193 @default.
• W4324349380 creator A5035851309 @default.
• W4324349380 creator A5078483539 @default.
• W4324349380 date "2023-03-15" @default.
• W4324349380 modified "2023-10-05" @default.
• W4324349380 title "A thermodynamic analysis of CLC transporter dimerization in lipid bilayers" @default.
• W4324349380 cites W187801720 @default.
• W4324349380 cites W1965335626 @default.
• W4324349380 cites W1997034145 @default.
• W4324349380 cites W1997501696 @default.
• W4324349380 cites W1999383818 @default.
• W4324349380 cites W2012218514 @default.
• W4324349380 cites W2017015733 @default.
• W4324349380 cites W2027711565 @default.
• W4324349380 cites W2037894649 @default.
• W4324349380 cites W2049097938 @default.
• W4324349380 cites W2052351570 @default.
• W4324349380 cites W2061510625 @default.
• W4324349380 cites W2068948441 @default.
• W4324349380 cites W2070732379 @default.
• W4324349380 cites W2074252493 @default.
• W4324349380 cites W2075705506 @default.
• W4324349380 cites W2080861274 @default.
• W4324349380 cites W2094462399 @default.
• W4324349380 cites W2151769926 @default.
• W4324349380 cites W2164797647 @default.
• W4324349380 cites W2278941722 @default.
• W4324349380 cites W2334577103 @default.
• W4324349380 cites W2475459768 @default.
• W4324349380 cites W2558276532 @default.
• W4324349380 cites W2950089318 @default.
• W4324349380 cites W3111500885 @default.
• W4324349380 cites W3117538715 @default.
• W4324349380 cites W3131658164 @default.
• W4324349380 cites W3146791856 @default.
• W4324349380 cites W380346485 @default.
• W4324349380 doi "https://doi.org/10.1101/2023.03.14.532678" @default.
• W4324349380 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36993257" @default.
• W4324349380 hasPublicationYear "2023" @default.
• W4324349380 type Work @default.
• W4324349380 citedByCount "1" @default.
• W4324349380 countsByYear W43243493802023 @default.
• W4324349380 crossrefType "posted-content" @default.
• W4324349380 hasAuthorship W4324349380A5006202510 @default.
• W4324349380 hasAuthorship W4324349380A5022192946 @default.
• W4324349380 hasAuthorship W4324349380A5026527193 @default.
• W4324349380 hasAuthorship W4324349380A5035851309 @default.
• W4324349380 hasAuthorship W4324349380A5078483539 @default.
• W4324349380 hasBestOaLocation W43243493801 @default.
• W4324349380 hasConcept C104292427 @default.
• W4324349380 hasConcept C104317684 @default.
• W4324349380 hasConcept C121332964 @default.
• W4324349380 hasConcept C12554922 @default.
• W4324349380 hasConcept C148898269 @default.
• W4324349380 hasConcept C15744967 @default.
• W4324349380 hasConcept C159467904 @default.
• W4324349380 hasConcept C166940927 @default.
• W4324349380 hasConcept C178790620 @default.
• W4324349380 hasConcept C185592680 @default.
• W4324349380 hasConcept C192157962 @default.
• W4324349380 hasConcept C2776029896 @default.
• W4324349380 hasConcept C32909587 @default.
• W4324349380 hasConcept C39944091 @default.
• W4324349380 hasConcept C41625074 @default.
• W4324349380 hasConcept C521977710 @default.
• W4324349380 hasConcept C55493867 @default.
• W4324349380 hasConcept C62520636 @default.
• W4324349380 hasConcept C77805123 @default.
• W4324349380 hasConcept C86803240 @default.
• W4324349380 hasConceptScore W4324349380C104292427 @default.
• W4324349380 hasConceptScore W4324349380C104317684 @default.
• W4324349380 hasConceptScore W4324349380C121332964 @default.
• W4324349380 hasConceptScore W4324349380C12554922 @default.
• W4324349380 hasConceptScore W4324349380C148898269 @default.
• W4324349380 hasConceptScore W4324349380C15744967 @default.
• W4324349380 hasConceptScore W4324349380C159467904 @default.
• W4324349380 hasConceptScore W4324349380C166940927 @default.
• W4324349380 hasConceptScore W4324349380C178790620 @default.
• W4324349380 hasConceptScore W4324349380C185592680 @default.
• W4324349380 hasConceptScore W4324349380C192157962 @default.
• W4324349380 hasConceptScore W4324349380C2776029896 @default.
• W4324349380 hasConceptScore W4324349380C32909587 @default.
• W4324349380 hasConceptScore W4324349380C39944091 @default.
• W4324349380 hasConceptScore W4324349380C41625074 @default.
• W4324349380 hasConceptScore W4324349380C521977710 @default.
• W4324349380 hasConceptScore W4324349380C55493867 @default.
• W4324349380 hasConceptScore W4324349380C62520636 @default.
• W4324349380 hasConceptScore W4324349380C77805123 @default.
• W4324349380 hasConceptScore W4324349380C86803240 @default.
• W4324349380 hasLocation W43243493801 @default.
• W4324349380 hasLocation W43243493802 @default.
• W4324349380 hasOpenAccess W4324349380 @default.
• W4324349380 hasPrimaryLocation W43243493801 @default.
• W4324349380 hasRelatedWork W1993362267 @default.
• W4324349380 hasRelatedWork W2021006840 @default.
• W4324349380 hasRelatedWork W2052772349 @default.

• next