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• W4324377422 abstract "Insertion sequences (IS) are compact and pervasive transposable elements found in bacteria, which encode only the genes necessary for their mobilization and maintenance. IS 200 /IS 605 elements undergo ‘peel-and-paste’ transposition catalyzed by a TnpA transposase, but intriguingly, they also encode diverse, TnpB- and IscB-family proteins that are evolutionarily related to the CRISPR-associated effectors Cas12 and Cas9, respectively. Recent studies demonstrated that TnpB-family enzymes function as RNA-guided DNA endonucleases, but the broader biological role of this activity has remained enigmatic. Here we show that TnpB/IscB are essential to prevent permanent transposon loss as a consequence of the TnpA transposition mechanism. We selected a family of related IS elements from Geobacillus stearothermophilus that encode diverse TnpB/IscB orthologs, and showed that a single TnpA transposase was active for transposon excision. The donor joints formed upon religation of IS-flanking sequences were efficiently targeted for cleavage by RNA-guided TnpB/IscB nucleases, and co-expression of TnpB together with TnpA led to significantly greater transposon retention, relative to conditions in which TnpA was expressed alone. Remarkably, TnpA and TnpB/IscB recognize the same AT-rich transposon-adjacent motif (TAM) during transposon excision and RNA-guided DNA cleavage, respectively, revealing a striking convergence in the evolution of DNA sequence specificity between collaborating transposase and nuclease proteins. Collectively, our study reveals that RNA-guided DNA cleavage is a primal biochemical activity that arose to bias the selfish inheritance and spread of transposable elements, which was later co-opted during the evolution of CRISPR-Cas adaptive immunity for antiviral defense." @default.
• W4324377422 created "2023-03-16" @default.
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• W4324377422 date "2023-03-14" @default.
• W4324377422 modified "2023-10-15" @default.
• W4324377422 title "Transposon-encoded nucleases use guide RNAs to selfishly bias their inheritance" @default.
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• W4324377422 cites W1975751378 @default.
• W4324377422 cites W1976907618 @default.
• W4324377422 cites W1988515582 @default.
• W4324377422 cites W2003845426 @default.
• W4324377422 cites W2005634944 @default.
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• W4324377422 doi "https://doi.org/10.1101/2023.03.14.532601" @default.
• W4324377422 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36993599" @default.
• W4324377422 hasPublicationYear "2023" @default.
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