Matches in SemOpenAlex for { <https://semopenalex.org/work/W4327559550> ?p ?o ?g. }
- W4327559550 endingPage "168048" @default.
- W4327559550 startingPage "168048" @default.
- W4327559550 abstract "Knr4/Smi1 proteins are specific to the fungal kingdom and their deletion in the model yeast Saccharomyces cerevisiae and the human pathogen Candida albicans results in hypersensitivity to specific antifungal agents and a wide range of parietal stresses. In S. cerevisiae, Knr4 is located at the crossroads of several signalling pathways, including the conserved cell wall integrity and calcineurin pathways. Knr4 interacts genetically and physically with several protein members of those pathways. Its sequence suggests that it contains large intrinsically disordered regions. Here, a combination of small-angle X-ray scattering (SAXS) and crystallographic analysis led to a comprehensive structural view of Knr4. This experimental work unambiguously showed that Knr4 comprises two large intrinsically disordered regions flanking a central globular domain whose structure has been established. The structured domain is itself interrupted by a disordered loop. Using the CRISPR/Cas9 genome editing technique, strains expressing KNR4 genes deleted from different domains were constructed. The N-terminal domain and the loop are essential for optimal resistance to cell wall-binding stressors. The C-terminal disordered domain, on the other hand, acts as a negative regulator of this function of Knr4. The identification of molecular recognition features, the possible presence of secondary structure in these disordered domains and the functional importance of the disordered domains revealed here designate these domains as putative interacting spots with partners in either pathway. Targeting these interacting regions is a promising route to the discovery of inhibitory molecules that could increase the susceptibility of pathogens to the antifungals currently in clinical use." @default.
- W4327559550 created "2023-03-17" @default.
- W4327559550 creator A5003178027 @default.
- W4327559550 creator A5006237770 @default.
- W4327559550 creator A5013235410 @default.
- W4327559550 creator A5017563952 @default.
- W4327559550 creator A5037282118 @default.
- W4327559550 creator A5044516864 @default.
- W4327559550 creator A5053108307 @default.
- W4327559550 creator A5065745586 @default.
- W4327559550 creator A5066210282 @default.
- W4327559550 date "2023-05-01" @default.
- W4327559550 modified "2023-10-18" @default.
- W4327559550 title "The Conserved Yeast Protein Knr4 Involved in Cell Wall Integrity Is a Multi-domain Intrinsically Disordered Protein" @default.
- W4327559550 cites W1481725570 @default.
- W4327559550 cites W1587918801 @default.
- W4327559550 cites W1740871519 @default.
- W4327559550 cites W1781742152 @default.
- W4327559550 cites W1797012724 @default.
- W4327559550 cites W1903792504 @default.
- W4327559550 cites W19446119 @default.
- W4327559550 cites W1975121208 @default.
- W4327559550 cites W1975395464 @default.
- W4327559550 cites W1979246102 @default.
- W4327559550 cites W1981346588 @default.
- W4327559550 cites W1984084102 @default.
- W4327559550 cites W2000639895 @default.
- W4327559550 cites W2000959229 @default.
- W4327559550 cites W2001843051 @default.
- W4327559550 cites W2005293003 @default.
- W4327559550 cites W2010412567 @default.
- W4327559550 cites W2015115860 @default.
- W4327559550 cites W2020940102 @default.
- W4327559550 cites W2021033050 @default.
- W4327559550 cites W2024912628 @default.
- W4327559550 cites W2035503835 @default.
- W4327559550 cites W2041728208 @default.
- W4327559550 cites W2042658485 @default.
- W4327559550 cites W2042745266 @default.
- W4327559550 cites W2053278954 @default.
- W4327559550 cites W2065304353 @default.
- W4327559550 cites W2066158357 @default.
- W4327559550 cites W2068501150 @default.
- W4327559550 cites W2072654958 @default.
- W4327559550 cites W2085277871 @default.
- W4327559550 cites W2097891553 @default.
- W4327559550 cites W2102186874 @default.
- W4327559550 cites W2102701612 @default.
- W4327559550 cites W2107620449 @default.
- W4327559550 cites W2108921801 @default.
- W4327559550 cites W2119489910 @default.
- W4327559550 cites W2124026197 @default.
- W4327559550 cites W2125668668 @default.
- W4327559550 cites W2130479394 @default.
- W4327559550 cites W2133102806 @default.
- W4327559550 cites W2138910991 @default.
- W4327559550 cites W2142351659 @default.
- W4327559550 cites W2143399071 @default.
- W4327559550 cites W2144363281 @default.
- W4327559550 cites W2150172960 @default.
- W4327559550 cites W2152812100 @default.
- W4327559550 cites W2157937554 @default.
- W4327559550 cites W2159460045 @default.
- W4327559550 cites W2165271176 @default.
- W4327559550 cites W2165581033 @default.
- W4327559550 cites W2170764510 @default.
- W4327559550 cites W2172196619 @default.
- W4327559550 cites W2178290447 @default.
- W4327559550 cites W2180229411 @default.
- W4327559550 cites W2300545781 @default.
- W4327559550 cites W2319761515 @default.
- W4327559550 cites W2346627216 @default.
- W4327559550 cites W2404834517 @default.
- W4327559550 cites W2515213957 @default.
- W4327559550 cites W2707646777 @default.
- W4327559550 cites W2766823907 @default.
- W4327559550 cites W2781927107 @default.
- W4327559550 cites W2793630394 @default.
- W4327559550 cites W2899330257 @default.
- W4327559550 cites W2899332527 @default.
- W4327559550 cites W2961108524 @default.
- W4327559550 cites W2969401397 @default.
- W4327559550 cites W2972974767 @default.
- W4327559550 cites W2977366709 @default.
- W4327559550 cites W3045283081 @default.
- W4327559550 cites W4224270926 @default.
- W4327559550 cites W4224312417 @default.
- W4327559550 cites W4248872320 @default.
- W4327559550 cites W4294316928 @default.
- W4327559550 doi "https://doi.org/10.1016/j.jmb.2023.168048" @default.
- W4327559550 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36933821" @default.
- W4327559550 hasPublicationYear "2023" @default.
- W4327559550 type Work @default.
- W4327559550 citedByCount "0" @default.
- W4327559550 crossrefType "journal-article" @default.
- W4327559550 hasAuthorship W4327559550A5003178027 @default.
- W4327559550 hasAuthorship W4327559550A5006237770 @default.
- W4327559550 hasAuthorship W4327559550A5013235410 @default.