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- W4327624477 endingPage "598" @default.
- W4327624477 startingPage "598" @default.
- W4327624477 abstract "The presence of a worn surface in the implanted material, as in the case of a replacement of a damaged osteoarticular joint, is the normal condition after implantation. This manuscript focuses precisely on the comparative study of the cellular behavior on worn CoCr surfaces, analyzing the effect of different surface modifications on macrophages’ responses. CoCr surfaces were modified by the deposition of electrochemically reduced graphene oxide (CoCrErGO), followed by additional surface functionalization with hyaluronic acid (CoCrErGOHA). After the wear corrosion processes, the macrophage response was studied. In addition, macrophage supernatants exposed to the surfaces, before and after wear, were also evaluated for osteoblast response through the analysis of the metabolic activity, plasma membrane damage, and phosphatase alkaline activity (ALP). The proteomic analysis and the quantitative TNF-α/IL-10 ratios of the J774A.1 macrophages exposed to the surfaces under study showed a polarization shift from M0 (basal state) to M1, associated with the pro-inflammatory response of all surfaces. A lower M1 polarization was observed upon exposure to the surface modification with ErGO, whereas posterior HA functionalization attenuated, even more, the M1 polarization. The wear corrosion process contributed to inflammation and exacerbated the M1 polarization response on macrophages to CoCr, which was diminished for the ErGO and attenuated the most for the ErGOHA surfaces. Comparative proteomics showed that the pathways related to M1 polarization were downregulated on the surfaces of CoCrErGOHA, which suggests mechanisms for the observed attenuation of M1 polarization. The suitable immuno-modulatory potential induced by the ErGOHA surface, with and without wear, together with the stimulation of ALP activity in osteoblasts induced by macrophage supernatants, promotes the mineralization processes necessary for bone repair. This makes it feasible to consider the adsorption of ErGOHA on CoCr as a recommended surface treatment for the use of biomaterials in osseous joint applications." @default.
- W4327624477 created "2023-03-17" @default.
- W4327624477 creator A5004279761 @default.
- W4327624477 creator A5006636225 @default.
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- W4327624477 creator A5077918173 @default.
- W4327624477 date "2023-03-15" @default.
- W4327624477 modified "2023-10-14" @default.
- W4327624477 title "Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages" @default.
- W4327624477 cites W1149703769 @default.
- W4327624477 cites W1521439356 @default.
- W4327624477 cites W1674731755 @default.
- W4327624477 cites W1797422316 @default.
- W4327624477 cites W1849819493 @default.
- W4327624477 cites W1977334935 @default.
- W4327624477 cites W1978363136 @default.
- W4327624477 cites W2005200593 @default.
- W4327624477 cites W2006315282 @default.
- W4327624477 cites W2008962117 @default.
- W4327624477 cites W2019286572 @default.
- W4327624477 cites W2023863818 @default.
- W4327624477 cites W2030730002 @default.
- W4327624477 cites W2032048503 @default.
- W4327624477 cites W2041539020 @default.
- W4327624477 cites W2042877041 @default.
- W4327624477 cites W2044020235 @default.
- W4327624477 cites W2053943711 @default.
- W4327624477 cites W2054789114 @default.
- W4327624477 cites W2076935230 @default.
- W4327624477 cites W2077637800 @default.
- W4327624477 cites W2083175247 @default.
- W4327624477 cites W2083415995 @default.
- W4327624477 cites W2083883672 @default.
- W4327624477 cites W2085783349 @default.
- W4327624477 cites W2088113721 @default.
- W4327624477 cites W2097732899 @default.
- W4327624477 cites W2103018087 @default.
- W4327624477 cites W2103907900 @default.
- W4327624477 cites W2126647999 @default.
- W4327624477 cites W2138469955 @default.
- W4327624477 cites W2140313746 @default.
- W4327624477 cites W2145237811 @default.
- W4327624477 cites W2156414024 @default.
- W4327624477 cites W2167760404 @default.
- W4327624477 cites W2195285929 @default.
- W4327624477 cites W2288852195 @default.
- W4327624477 cites W2308058717 @default.
- W4327624477 cites W2319967189 @default.
- W4327624477 cites W2400239628 @default.
- W4327624477 cites W2409248716 @default.
- W4327624477 cites W2471800028 @default.
- W4327624477 cites W2512119505 @default.
- W4327624477 cites W2547069175 @default.
- W4327624477 cites W2589392019 @default.
- W4327624477 cites W2602055674 @default.
- W4327624477 cites W2624317658 @default.
- W4327624477 cites W2735527928 @default.
- W4327624477 cites W2772588876 @default.
- W4327624477 cites W2775854361 @default.
- W4327624477 cites W2784042879 @default.
- W4327624477 cites W2790990555 @default.
- W4327624477 cites W2793818171 @default.
- W4327624477 cites W2794818311 @default.
- W4327624477 cites W2795915345 @default.
- W4327624477 cites W2802486617 @default.
- W4327624477 cites W2804707443 @default.
- W4327624477 cites W2888450103 @default.
- W4327624477 cites W2892543629 @default.
- W4327624477 cites W2941157893 @default.
- W4327624477 cites W2945428791 @default.
- W4327624477 cites W2952534170 @default.
- W4327624477 cites W2970944213 @default.
- W4327624477 cites W2990398858 @default.
- W4327624477 cites W2993054314 @default.
- W4327624477 cites W2997022986 @default.
- W4327624477 cites W2998098744 @default.
- W4327624477 cites W3007280959 @default.
- W4327624477 cites W3010418854 @default.
- W4327624477 cites W3014735844 @default.
- W4327624477 cites W3016881059 @default.
- W4327624477 cites W3025502520 @default.
- W4327624477 cites W3041758973 @default.
- W4327624477 cites W3044606159 @default.
- W4327624477 cites W3086022822 @default.
- W4327624477 cites W3089296243 @default.
- W4327624477 cites W3091534581 @default.
- W4327624477 cites W3105411011 @default.
- W4327624477 cites W3117742513 @default.
- W4327624477 cites W3146582890 @default.
- W4327624477 cites W3146754033 @default.
- W4327624477 cites W3152719213 @default.
- W4327624477 cites W3157638995 @default.
- W4327624477 cites W3165615241 @default.
- W4327624477 cites W3178354155 @default.