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• W4327702781 abstract "Alkaline phosphatase (ALP), as a crucial enzyme involved in many physiological activities, is always used as one of the significant biomarkers in clinical diagnosis. Herein, a novel, simple, and effective photothermal quantitative method based on the etching of MnO2-coated gold nanoparticles (Au@MnO2 NPs) was established for ALP activity assay with a household thermometer-based visual readout. The photothermal effect of Au@MnO2 NPs is much higher than that of MnO2 NPs or Au NPs. The MnO2 shell of Au@MnO2 NPs can be etched by ascorbic acid, a product of ALP-catalyzed hydrolysis of 2-phospho-l-ascorbic acid. With the etching of Au@MnO2 NPs, the photothermal conversion efficiency decreased gradually, causing the decrease of the temperature increment of the solutions by degrees. A household thermometer, instead of large-scale and professional instruments, was used as a signal reader to realize the visual quantitative detection. The photothermal platform was used successfully for the determination of ALP with a wide linear range from 2.0 to 50 U/L and a detection limit as low as 0.75 U/L. Moreover, the inhibition efficiency of sodium vanadate for ALP activity was investigated, proving the photothermal quantitative method will be a potential platform for screening enzyme inhibitors. Such a sensitive, facile, and low-cost sensing assay provides a new prospect to develop platforms for point-of-care testing." @default.
• W4327702781 created "2023-03-18" @default.
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• W4327702781 date "2023-07-01" @default.
• W4327702781 modified "2023-10-18" @default.
• W4327702781 title "Photothermal visual sensing of alkaline phosphatase based on the etching of Au@MnO2 core-shell nanoparticles" @default.
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• W4327702781 doi "https://doi.org/10.1016/j.jcis.2023.03.091" @default.
• W4327702781 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36963250" @default.
• W4327702781 hasPublicationYear "2023" @default.
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