Matches in SemOpenAlex for { <https://semopenalex.org/work/W4327737133> ?p ?o ?g. }
- W4327737133 endingPage "5692" @default.
- W4327737133 startingPage "5692" @default.
- W4327737133 abstract "Scaffolds made of degradable polymers, such as collagen, polyesters or polysaccharides, are promising matrices for fabrication of bioartificial vascular grafts or patches. In this study, collagen isolated from porcine skin was processed into a gel, reinforced with collagen particles and with incorporated adipose tissue-derived stem cells (ASCs). The cell-material constructs were then incubated in a DMEM medium with 2% of FS (DMEM_part), with added polyvinylalcohol nanofibers (PVA_part sample), and for ASCs differentiation towards smooth muscle cells (SMCs), the medium was supplemented either with human platelet lysate released from PVA nanofibers (PVA_PL_part) or with TGF-β1 + BMP-4 (TGF + BMP_part). The constructs were further endothelialised with human umbilical vein endothelial cells (ECs). The immunofluorescence staining of alpha-actin and calponin, and von Willebrand factor, was performed. The proteins involved in cell differentiation, the extracellular matrix (ECM) proteins, and ECM remodelling proteins were evaluated by mass spectrometry on day 12 of culture. Mechanical properties of the gels with ASCs were measured via an unconfined compression test on day 5. Gels evinced limited planar shrinkage, but it was higher in endothelialised TGF + BMP_part gel. Both PVA_PL_part samples and TGF + BMP_part samples supported ASC growth and differentiation towards SMCs, but only PVA_PL_part supported homogeneous endothelialisation. Young modulus of elasticity increased in all samples compared to day 0, and PVA_PL_part gel evinced a slightly higher ratio of elastic energy. The results suggest that PVA_PL_part collagen construct has the highest potential to remodel into a functional vascular wall." @default.
- W4327737133 created "2023-03-18" @default.
- W4327737133 creator A5002185322 @default.
- W4327737133 creator A5004657631 @default.
- W4327737133 creator A5009609479 @default.
- W4327737133 creator A5018302877 @default.
- W4327737133 creator A5021100757 @default.
- W4327737133 creator A5024442513 @default.
- W4327737133 creator A5027971675 @default.
- W4327737133 creator A5029973675 @default.
- W4327737133 creator A5036721132 @default.
- W4327737133 creator A5043945805 @default.
- W4327737133 creator A5045679649 @default.
- W4327737133 creator A5053410783 @default.
- W4327737133 creator A5053848773 @default.
- W4327737133 creator A5056911997 @default.
- W4327737133 creator A5062530795 @default.
- W4327737133 creator A5068418207 @default.
- W4327737133 creator A5070693848 @default.
- W4327737133 creator A5089655445 @default.
- W4327737133 creator A5090293257 @default.
- W4327737133 date "2023-03-16" @default.
- W4327737133 modified "2023-10-18" @default.
- W4327737133 title "Adipose-Derived Stem Cells in Reinforced Collagen Gel: A Comparison between Two Approaches to Differentiation towards Smooth Muscle Cells" @default.
- W4327737133 cites W1905619727 @default.
- W4327737133 cites W1965942829 @default.
- W4327737133 cites W1967561815 @default.
- W4327737133 cites W1972468602 @default.
- W4327737133 cites W1976604464 @default.
- W4327737133 cites W1976742584 @default.
- W4327737133 cites W1977615915 @default.
- W4327737133 cites W1982019332 @default.
- W4327737133 cites W1983341185 @default.
- W4327737133 cites W1986743279 @default.
- W4327737133 cites W1988613013 @default.
- W4327737133 cites W1994979643 @default.
- W4327737133 cites W1996887121 @default.
- W4327737133 cites W1999596056 @default.
- W4327737133 cites W2001766687 @default.
- W4327737133 cites W2018028722 @default.
- W4327737133 cites W2025023666 @default.
- W4327737133 cites W2026100597 @default.
- W4327737133 cites W2046656259 @default.
- W4327737133 cites W2052426301 @default.
- W4327737133 cites W2065264912 @default.
- W4327737133 cites W2077833708 @default.
- W4327737133 cites W2080727386 @default.
- W4327737133 cites W2081146929 @default.
- W4327737133 cites W2089572048 @default.
- W4327737133 cites W2111806914 @default.
- W4327737133 cites W2130477118 @default.
- W4327737133 cites W2147647689 @default.
- W4327737133 cites W2154018739 @default.
- W4327737133 cites W2234536314 @default.
- W4327737133 cites W2347012456 @default.
- W4327737133 cites W2467927036 @default.
- W4327737133 cites W2519829326 @default.
- W4327737133 cites W2530528982 @default.
- W4327737133 cites W2554900211 @default.
- W4327737133 cites W2582816205 @default.
- W4327737133 cites W2586129172 @default.
- W4327737133 cites W2591256387 @default.
- W4327737133 cites W2620631111 @default.
- W4327737133 cites W2717381632 @default.
- W4327737133 cites W2740397159 @default.
- W4327737133 cites W2754921291 @default.
- W4327737133 cites W2769245037 @default.
- W4327737133 cites W2769533324 @default.
- W4327737133 cites W2789930460 @default.
- W4327737133 cites W2800659616 @default.
- W4327737133 cites W2801929954 @default.
- W4327737133 cites W2803531651 @default.
- W4327737133 cites W2884506035 @default.
- W4327737133 cites W2888206054 @default.
- W4327737133 cites W2904329190 @default.
- W4327737133 cites W2918000376 @default.
- W4327737133 cites W2926970134 @default.
- W4327737133 cites W2954403770 @default.
- W4327737133 cites W2958862454 @default.
- W4327737133 cites W3004878409 @default.
- W4327737133 cites W3007051837 @default.
- W4327737133 cites W3031901586 @default.
- W4327737133 cites W3034819588 @default.
- W4327737133 cites W3046673147 @default.
- W4327737133 cites W3048125906 @default.
- W4327737133 cites W3083032305 @default.
- W4327737133 cites W3088444589 @default.
- W4327737133 cites W3090525150 @default.
- W4327737133 cites W3111095888 @default.
- W4327737133 cites W3119987757 @default.
- W4327737133 cites W3125316908 @default.
- W4327737133 cites W3133825917 @default.
- W4327737133 cites W3156690633 @default.
- W4327737133 cites W3156832860 @default.
- W4327737133 cites W3181869496 @default.
- W4327737133 cites W3213164535 @default.
- W4327737133 cites W4200182990 @default.
- W4327737133 cites W4200462317 @default.