FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4327738971> ?p ?o ?g. }

• W4327738971 abstract "Tumor cell density is a basic pathological feature of solid tumors. Chemotherapy, radiotherapy, and targeted therapy reduce tumor cell density, whereas unrestricted tumor cell proliferation promotes this feature. The impact of tumor cells on the microenvironment following changes in tumor cell density is still unclear. In this study, we focused on the response of key immune cell subsets to tumor cell density in hepatocellular carcinoma (HCC).We determined the density of tumor and immune cells in the same area by section staining. We then identified potential mediators using polymerase chain reaction (PCR), enzyme-linked immunofluorescence assay (ELISA), 3D and co-culture, flow cytometry, and lentivirus intervention. The mechanism of lactate promotion was verified using lactate tests, bioinformatics, western blotting, and the above methods. The IL-8/DAPK1/lactate/regulatory T cell (Treg) axis was verified using a mouse liver cancer model. Tumor mutation burden was calculated using maftools in R.We found that the Treg/CD8 + T cell ratio is not consistent with tumor cell density in HCC, and a decreased Treg/CD8 + T cell ratio in the range of 5000-6000 cells/mm2 may elicit the possibility for immunotherapy in an immunosuppressive microenvironment. We showed that IL-8 mediates this immune fluctuation and promotes the infiltration of Tregs through the DAPK1/pyruvate kinase activity/lactate axis in HCC. Based on tumor ploidy and mutation burden data, we discussed the potential significance of immune fluctuation in the homeostasis of HCC mutation burden and proposed a density checkpoint and entropy model to describe this phenomenon.In summary, we report the mode of infiltration of Tregs/CD8 + T cells in response to tumor cell density and provide a new theoretical basis for IL-8 as a therapeutic target and the selection of an immunotherapy window in HCC." @default.
• W4327738971 created "2023-03-18" @default.
• W4327738971 creator A5015640756 @default.
• W4327738971 creator A5025484377 @default.
• W4327738971 creator A5030929563 @default.
• W4327738971 creator A5035026050 @default.
• W4327738971 creator A5049974343 @default.
• W4327738971 creator A5051742732 @default.
• W4327738971 creator A5067123604 @default.
• W4327738971 creator A5069001172 @default.
• W4327738971 creator A5079876311 @default.
• W4327738971 date "2023-03-17" @default.
• W4327738971 modified "2023-10-16" @default.
• W4327738971 title "Tumor cell density dependent IL-8 secretion induces the fluctuation of tregs/CD8 + T cells infiltration in hepatocellular carcinoma: one prompt for the existence of density checkpoint" @default.
• W4327738971 cites W1539219439 @default.
• W4327738971 cites W1964167504 @default.
• W4327738971 cites W1965065360 @default.
• W4327738971 cites W1970915918 @default.
• W4327738971 cites W1985357425 @default.
• W4327738971 cites W1996646041 @default.
• W4327738971 cites W2004762679 @default.
• W4327738971 cites W2015207840 @default.
• W4327738971 cites W2023006686 @default.
• W4327738971 cites W2025140437 @default.
• W4327738971 cites W2039558597 @default.
• W4327738971 cites W2059821930 @default.
• W4327738971 cites W2107336310 @default.
• W4327738971 cites W2111130690 @default.
• W4327738971 cites W2122656254 @default.
• W4327738971 cites W2143172913 @default.
• W4327738971 cites W2155892126 @default.
• W4327738971 cites W2205830781 @default.
• W4327738971 cites W2288773605 @default.
• W4327738971 cites W2298626553 @default.
• W4327738971 cites W2344995323 @default.
• W4327738971 cites W2396860769 @default.
• W4327738971 cites W2468308090 @default.
• W4327738971 cites W2512931560 @default.
• W4327738971 cites W2587610309 @default.
• W4327738971 cites W2606156013 @default.
• W4327738971 cites W2620210866 @default.
• W4327738971 cites W2768908212 @default.
• W4327738971 cites W2791378117 @default.
• W4327738971 cites W2810661705 @default.
• W4327738971 cites W2887408433 @default.
• W4327738971 cites W2911918039 @default.
• W4327738971 cites W2912815018 @default.
• W4327738971 cites W3089199226 @default.
• W4327738971 cites W3097531118 @default.
• W4327738971 cites W3097803258 @default.
• W4327738971 cites W3106842458 @default.
• W4327738971 cites W3109260033 @default.
• W4327738971 cites W3128646645 @default.
• W4327738971 cites W3133076643 @default.
• W4327738971 cites W3139219703 @default.
• W4327738971 cites W3156292065 @default.
• W4327738971 cites W3157850464 @default.
• W4327738971 cites W3197187837 @default.
• W4327738971 cites W4298146998 @default.
• W4327738971 doi "https://doi.org/10.1186/s12967-023-04060-3" @default.
• W4327738971 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36932390" @default.
• W4327738971 hasPublicationYear "2023" @default.
• W4327738971 type Work @default.
• W4327738971 citedByCount "0" @default.
• W4327738971 crossrefType "journal-article" @default.
• W4327738971 hasAuthorship W4327738971A5015640756 @default.
• W4327738971 hasAuthorship W4327738971A5025484377 @default.
• W4327738971 hasAuthorship W4327738971A5030929563 @default.
• W4327738971 hasAuthorship W4327738971A5035026050 @default.
• W4327738971 hasAuthorship W4327738971A5049974343 @default.
• W4327738971 hasAuthorship W4327738971A5051742732 @default.
• W4327738971 hasAuthorship W4327738971A5067123604 @default.
• W4327738971 hasAuthorship W4327738971A5069001172 @default.
• W4327738971 hasAuthorship W4327738971A5079876311 @default.
• W4327738971 hasBestOaLocation W43277389711 @default.
• W4327738971 hasConcept C1491633281 @default.
• W4327738971 hasConcept C167672396 @default.
• W4327738971 hasConcept C185592680 @default.
• W4327738971 hasConcept C203014093 @default.
• W4327738971 hasConcept C2776090121 @default.
• W4327738971 hasConcept C2776107976 @default.
• W4327738971 hasConcept C2777701055 @default.
• W4327738971 hasConcept C2778019345 @default.
• W4327738971 hasConcept C502942594 @default.
• W4327738971 hasConcept C54355233 @default.
• W4327738971 hasConcept C553184892 @default.
• W4327738971 hasConcept C86803240 @default.
• W4327738971 hasConcept C8891405 @default.
• W4327738971 hasConceptScore W4327738971C1491633281 @default.
• W4327738971 hasConceptScore W4327738971C167672396 @default.
• W4327738971 hasConceptScore W4327738971C185592680 @default.
• W4327738971 hasConceptScore W4327738971C203014093 @default.
• W4327738971 hasConceptScore W4327738971C2776090121 @default.
• W4327738971 hasConceptScore W4327738971C2776107976 @default.
• W4327738971 hasConceptScore W4327738971C2777701055 @default.
• W4327738971 hasConceptScore W4327738971C2778019345 @default.
• W4327738971 hasConceptScore W4327738971C502942594 @default.
• W4327738971 hasConceptScore W4327738971C54355233 @default.
• W4327738971 hasConceptScore W4327738971C553184892 @default.
• W4327738971 hasConceptScore W4327738971C86803240 @default.

• next