FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4327898714> ?p ?o ?g. }

• W4327898714 endingPage "2418" @default.
• W4327898714 startingPage "2403" @default.
• W4327898714 abstract "Abstract The integration of a transgene expression construct into the host genome is the initial step for the generation of recombinant cell lines used for biopharmaceutical production. The stability and level of recombinant gene expression in Chinese hamster ovary (CHO) can be correlated to the copy number, its integration site as well as the epigenetic context of the transgene vector. Also, undesired integration events, such as concatemers, truncated, and inverted vector repeats, are impacting the stability of recombinant cell lines. Thus, to characterize cell clones and to isolate the most promising candidates, it is crucial to obtain information on the site of integration, the structure of integrated sequence and the epigenetic status. Current sequencing techniques allow to gather this information separately but do not offer a comprehensive and simultaneous resolution. In this study, we present a fast and robust nanopore Cas9‐targeted sequencing (nCats) pipeline to identify integration sites, the composition of the integrated sequence as well as its DNA methylation status in CHO cells that can be obtained simultaneously from the same sequencing run. A Cas9‐enrichment step during library preparation enables targeted and directional nanopore sequencing with up to 724× median on‐target coverage and up to 153 kb long reads. The data generated by nCats provides sensitive, detailed, and correct information on the transgene integration sites and the expression vector structure, which could only be partly produced by traditional Targeted Locus Amplification‐seq data. Moreover, with nCats the DNA methylation status can be analyzed from the same raw data without prior DNA amplification." @default.
• W4327898714 created "2023-03-21" @default.
• W4327898714 creator A5011188986 @default.
• W4327898714 creator A5078670673 @default.
• W4327898714 creator A5079363697 @default.
• W4327898714 creator A5080556798 @default.
• W4327898714 date "2023-04-14" @default.
• W4327898714 modified "2023-10-14" @default.
• W4327898714 title "Nanopore Cas9‐targeted sequencing enables accurate and simultaneous identification of transgene integration sites, their structure and epigenetic status in recombinant Chinese hamster ovary cells" @default.
• W4327898714 cites W1996423252 @default.
• W4327898714 cites W2021341670 @default.
• W4327898714 cites W2036176224 @default.
• W4327898714 cites W2049974202 @default.
• W4327898714 cites W2055043387 @default.
• W4327898714 cites W2099259774 @default.
• W4327898714 cites W2102619694 @default.
• W4327898714 cites W2108234281 @default.
• W4327898714 cites W2115571948 @default.
• W4327898714 cites W2138270253 @default.
• W4327898714 cites W2150936392 @default.
• W4327898714 cites W2340837533 @default.
• W4327898714 cites W2388714865 @default.
• W4327898714 cites W2575750030 @default.
• W4327898714 cites W2591367881 @default.
• W4327898714 cites W2738247066 @default.
• W4327898714 cites W2760954512 @default.
• W4327898714 cites W2788228074 @default.
• W4327898714 cites W2789843538 @default.
• W4327898714 cites W2800755544 @default.
• W4327898714 cites W2803479625 @default.
• W4327898714 cites W2803643875 @default.
• W4327898714 cites W2804352314 @default.
• W4327898714 cites W2836977427 @default.
• W4327898714 cites W2887092751 @default.
• W4327898714 cites W2888734161 @default.
• W4327898714 cites W2901438556 @default.
• W4327898714 cites W2902516983 @default.
• W4327898714 cites W2916937267 @default.
• W4327898714 cites W2953981450 @default.
• W4327898714 cites W3004807164 @default.
• W4327898714 cites W3022783334 @default.
• W4327898714 cites W3024127014 @default.
• W4327898714 cites W3029256690 @default.
• W4327898714 cites W3042615841 @default.
• W4327898714 cites W3081088497 @default.
• W4327898714 cites W3103674725 @default.
• W4327898714 cites W3110462241 @default.
• W4327898714 cites W3112304822 @default.
• W4327898714 cites W3122603135 @default.
• W4327898714 cites W3131055609 @default.
• W4327898714 cites W3162567805 @default.
• W4327898714 cites W3170621487 @default.
• W4327898714 cites W4200360858 @default.
• W4327898714 cites W4205234050 @default.
• W4327898714 cites W4210835070 @default.
• W4327898714 cites W4220776860 @default.
• W4327898714 cites W4220999759 @default.
• W4327898714 cites W4225908069 @default.
• W4327898714 cites W4226000574 @default.
• W4327898714 cites W4304822964 @default.
• W4327898714 cites W4315619277 @default.
• W4327898714 cites W4324135855 @default.
• W4327898714 doi "https://doi.org/10.1002/bit.28382" @default.
• W4327898714 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36938677" @default.
• W4327898714 hasPublicationYear "2023" @default.
• W4327898714 type Work @default.
• W4327898714 citedByCount "1" @default.
• W4327898714 countsByYear W43278987142023 @default.
• W4327898714 crossrefType "journal-article" @default.
• W4327898714 hasAuthorship W4327898714A5011188986 @default.
• W4327898714 hasAuthorship W4327898714A5078670673 @default.
• W4327898714 hasAuthorship W4327898714A5079363697 @default.
• W4327898714 hasAuthorship W4327898714A5080556798 @default.
• W4327898714 hasBestOaLocation W43278987141 @default.
• W4327898714 hasConcept C102230213 @default.
• W4327898714 hasConcept C104317684 @default.
• W4327898714 hasConcept C126513998 @default.
• W4327898714 hasConcept C132455925 @default.
• W4327898714 hasConcept C150194340 @default.
• W4327898714 hasConcept C175656101 @default.
• W4327898714 hasConcept C190727270 @default.
• W4327898714 hasConcept C41091548 @default.
• W4327898714 hasConcept C51679486 @default.
• W4327898714 hasConcept C54355233 @default.
• W4327898714 hasConcept C70721500 @default.
• W4327898714 hasConcept C81885089 @default.
• W4327898714 hasConcept C86803240 @default.
• W4327898714 hasConcept C98108389 @default.
• W4327898714 hasConceptScore W4327898714C102230213 @default.
• W4327898714 hasConceptScore W4327898714C104317684 @default.
• W4327898714 hasConceptScore W4327898714C126513998 @default.
• W4327898714 hasConceptScore W4327898714C132455925 @default.
• W4327898714 hasConceptScore W4327898714C150194340 @default.
• W4327898714 hasConceptScore W4327898714C175656101 @default.
• W4327898714 hasConceptScore W4327898714C190727270 @default.
• W4327898714 hasConceptScore W4327898714C41091548 @default.
• W4327898714 hasConceptScore W4327898714C51679486 @default.
• W4327898714 hasConceptScore W4327898714C54355233 @default.

• next