Matches in SemOpenAlex for { <https://semopenalex.org/work/W4327909969> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W4327909969 abstract "Abstract Polymerase Chain Reaction (PCR) is one of the important techniques in molecular biology for many diagnostics and research applications. In this current theoretical PCR optimization research, out of its seven recipe reagents, two of the important constituents e.g., DNA which might be very rare in forensic cases and polymerase enzyme due to its high cost were mathematically modeled and computationally simulated using MATLAB programming platform. Chemical equations of PCR stages were established using the law of mass action followed by differential equations derivations from each of the DNA amplification phases. Firstly, a mathematical model of Taq polymerase enzyme with its variable concentrations ranging from 0.05-0.35 IU / μL was formulated which showed the best PCR product of 0.61 ng/μL obtained with 0.2 IU/μL of polymerase. Similarly, by fixing the optimal polymerase concentration obtained in the previous simulation, the prime-DNA variable concentrations ranging from 0.5-3.5 ng/μL were further simulated which showed the best PCR product of 0.94 ng/μL at 2.0 ng/μL of DNA. PCR wet-lab experimentation and gel-electrophoresis visualization validated mathematically simulated optimized concentrations of the aforementioned two reagents which vary the PCR product considerably. Current findings indicate that mathematically optimized concentrations of Taq -polymerase and template-DNA may reduce the PCR cost, time and energy, but other factors of target-sequence specificity, purity/concentrations of the other reagents, working hygiene, technical intricacies, biosafety and biosecurity factors may also contribute the desired yield of the amplicon." @default.
- W4327909969 created "2023-03-21" @default.
- W4327909969 creator A5010749699 @default.
- W4327909969 creator A5054617990 @default.
- W4327909969 creator A5070230738 @default.
- W4327909969 creator A5085855469 @default.
- W4327909969 date "2023-03-20" @default.
- W4327909969 modified "2023-09-27" @default.
- W4327909969 title "Mathematical Modeling of PCR with Variable Concentrations of DNA and<i>Taq</i>Polymerase" @default.
- W4327909969 cites W1968159228 @default.
- W4327909969 cites W2033793049 @default.
- W4327909969 cites W2039146500 @default.
- W4327909969 cites W2047375868 @default.
- W4327909969 cites W2100869063 @default.
- W4327909969 cites W2152177178 @default.
- W4327909969 cites W2154314575 @default.
- W4327909969 cites W2168870480 @default.
- W4327909969 cites W2255475584 @default.
- W4327909969 cites W2606646153 @default.
- W4327909969 doi "https://doi.org/10.1101/2023.03.16.532908" @default.
- W4327909969 hasPublicationYear "2023" @default.
- W4327909969 type Work @default.
- W4327909969 citedByCount "0" @default.
- W4327909969 crossrefType "posted-content" @default.
- W4327909969 hasAuthorship W4327909969A5010749699 @default.
- W4327909969 hasAuthorship W4327909969A5054617990 @default.
- W4327909969 hasAuthorship W4327909969A5070230738 @default.
- W4327909969 hasAuthorship W4327909969A5085855469 @default.
- W4327909969 hasBestOaLocation W43279099691 @default.
- W4327909969 hasConcept C104317684 @default.
- W4327909969 hasConcept C153911025 @default.
- W4327909969 hasConcept C157431215 @default.
- W4327909969 hasConcept C185592680 @default.
- W4327909969 hasConcept C186008079 @default.
- W4327909969 hasConcept C2756471 @default.
- W4327909969 hasConcept C2779186596 @default.
- W4327909969 hasConcept C2781440490 @default.
- W4327909969 hasConcept C28134486 @default.
- W4327909969 hasConcept C48023723 @default.
- W4327909969 hasConcept C49105822 @default.
- W4327909969 hasConcept C552990157 @default.
- W4327909969 hasConcept C55493867 @default.
- W4327909969 hasConcept C70721500 @default.
- W4327909969 hasConcept C8185291 @default.
- W4327909969 hasConcept C82381507 @default.
- W4327909969 hasConcept C86803240 @default.
- W4327909969 hasConcept C90583042 @default.
- W4327909969 hasConceptScore W4327909969C104317684 @default.
- W4327909969 hasConceptScore W4327909969C153911025 @default.
- W4327909969 hasConceptScore W4327909969C157431215 @default.
- W4327909969 hasConceptScore W4327909969C185592680 @default.
- W4327909969 hasConceptScore W4327909969C186008079 @default.
- W4327909969 hasConceptScore W4327909969C2756471 @default.
- W4327909969 hasConceptScore W4327909969C2779186596 @default.
- W4327909969 hasConceptScore W4327909969C2781440490 @default.
- W4327909969 hasConceptScore W4327909969C28134486 @default.
- W4327909969 hasConceptScore W4327909969C48023723 @default.
- W4327909969 hasConceptScore W4327909969C49105822 @default.
- W4327909969 hasConceptScore W4327909969C552990157 @default.
- W4327909969 hasConceptScore W4327909969C55493867 @default.
- W4327909969 hasConceptScore W4327909969C70721500 @default.
- W4327909969 hasConceptScore W4327909969C8185291 @default.
- W4327909969 hasConceptScore W4327909969C82381507 @default.
- W4327909969 hasConceptScore W4327909969C86803240 @default.
- W4327909969 hasConceptScore W4327909969C90583042 @default.
- W4327909969 hasLocation W43279099691 @default.
- W4327909969 hasOpenAccess W4327909969 @default.
- W4327909969 hasPrimaryLocation W43279099691 @default.
- W4327909969 hasRelatedWork W1989695866 @default.
- W4327909969 hasRelatedWork W1999554970 @default.
- W4327909969 hasRelatedWork W2081860452 @default.
- W4327909969 hasRelatedWork W2164131361 @default.
- W4327909969 hasRelatedWork W2364228712 @default.
- W4327909969 hasRelatedWork W2400789298 @default.
- W4327909969 hasRelatedWork W2414933257 @default.
- W4327909969 hasRelatedWork W3147299065 @default.
- W4327909969 hasRelatedWork W4327909969 @default.
- W4327909969 hasRelatedWork W2954313502 @default.
- W4327909969 isParatext "false" @default.
- W4327909969 isRetracted "false" @default.
- W4327909969 workType "article" @default.