Matches in SemOpenAlex for { <https://semopenalex.org/work/W4327967069> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W4327967069 abstract "Designing and selecting cell culture media and their feeding are a key strategy to maximize culture performance in industrial biopharmaceutical processes. However, mammalian cells are very sensitive to their culture environment, requiring specific nutritional needs to grow and produce high-quality proteins such as antibodies, depending on cell lines and operational conditions. In this regard, previously we developed data-driven and in-silico model-guided systematic framework to investigate the effect of growth media on Chinese hamster ovary (CHO) cell culture performance, allowing us to design and reformulate basal media. To expand our exploration for media development research further, we evaluated two chemically defined feed media, A and B, in ambr15 bioreactor runs using a monoclonal antibody-producing CHO K1 cell line. We observed a significant impact of feed media on cell growth, longevity, viability, productivity and toxic metabolites production. Specifically, concentrated feed A was not sufficient to support prolonged cell culture and high titer compared to feed B. The framework systematically characterized the major metabolic bottlenecks in the TCA cycle and its related amino acid transferase reactions, thereby identifying key design components, such as asparagine, aspartate, and glutamate, which are needed for highly productive cell cultures. Based on our results, we subsequently reformulated the feeds by adjusting the amounts of those amino acids and successfully validated their effectiveness in promoting cell growth and/or titer." @default.
- W4327967069 created "2023-03-21" @default.
- W4327967069 creator A5006629240 @default.
- W4327967069 creator A5009094679 @default.
- W4327967069 creator A5011206845 @default.
- W4327967069 creator A5014656954 @default.
- W4327967069 creator A5029819149 @default.
- W4327967069 creator A5043271815 @default.
- W4327967069 creator A5051206694 @default.
- W4327967069 creator A5089375264 @default.
- W4327967069 date "2023-03-20" @default.
- W4327967069 modified "2023-09-30" @default.
- W4327967069 title "Debottlenecking and reformulating feed media for improved CHO cell growth and titer by data-driven and model-guided analyses" @default.
- W4327967069 doi "https://doi.org/10.22541/au.167930331.11784279/v1" @default.
- W4327967069 hasPublicationYear "2023" @default.
- W4327967069 type Work @default.
- W4327967069 citedByCount "0" @default.
- W4327967069 crossrefType "posted-content" @default.
- W4327967069 hasAuthorship W4327967069A5006629240 @default.
- W4327967069 hasAuthorship W4327967069A5009094679 @default.
- W4327967069 hasAuthorship W4327967069A5011206845 @default.
- W4327967069 hasAuthorship W4327967069A5014656954 @default.
- W4327967069 hasAuthorship W4327967069A5029819149 @default.
- W4327967069 hasAuthorship W4327967069A5043271815 @default.
- W4327967069 hasAuthorship W4327967069A5051206694 @default.
- W4327967069 hasAuthorship W4327967069A5089375264 @default.
- W4327967069 hasBestOaLocation W43279670691 @default.
- W4327967069 hasConcept C104317684 @default.
- W4327967069 hasConcept C150903083 @default.
- W4327967069 hasConcept C159654299 @default.
- W4327967069 hasConcept C175656101 @default.
- W4327967069 hasConcept C202751555 @default.
- W4327967069 hasConcept C203014093 @default.
- W4327967069 hasConcept C2775895851 @default.
- W4327967069 hasConcept C2775905019 @default.
- W4327967069 hasConcept C2779349466 @default.
- W4327967069 hasConcept C32611913 @default.
- W4327967069 hasConcept C515207424 @default.
- W4327967069 hasConcept C54355233 @default.
- W4327967069 hasConcept C55493867 @default.
- W4327967069 hasConcept C62112901 @default.
- W4327967069 hasConcept C70721500 @default.
- W4327967069 hasConcept C81885089 @default.
- W4327967069 hasConcept C86803240 @default.
- W4327967069 hasConcept C98648569 @default.
- W4327967069 hasConceptScore W4327967069C104317684 @default.
- W4327967069 hasConceptScore W4327967069C150903083 @default.
- W4327967069 hasConceptScore W4327967069C159654299 @default.
- W4327967069 hasConceptScore W4327967069C175656101 @default.
- W4327967069 hasConceptScore W4327967069C202751555 @default.
- W4327967069 hasConceptScore W4327967069C203014093 @default.
- W4327967069 hasConceptScore W4327967069C2775895851 @default.
- W4327967069 hasConceptScore W4327967069C2775905019 @default.
- W4327967069 hasConceptScore W4327967069C2779349466 @default.
- W4327967069 hasConceptScore W4327967069C32611913 @default.
- W4327967069 hasConceptScore W4327967069C515207424 @default.
- W4327967069 hasConceptScore W4327967069C54355233 @default.
- W4327967069 hasConceptScore W4327967069C55493867 @default.
- W4327967069 hasConceptScore W4327967069C62112901 @default.
- W4327967069 hasConceptScore W4327967069C70721500 @default.
- W4327967069 hasConceptScore W4327967069C81885089 @default.
- W4327967069 hasConceptScore W4327967069C86803240 @default.
- W4327967069 hasConceptScore W4327967069C98648569 @default.
- W4327967069 hasLocation W43279670691 @default.
- W4327967069 hasLocation W43279670692 @default.
- W4327967069 hasOpenAccess W4327967069 @default.
- W4327967069 hasPrimaryLocation W43279670691 @default.
- W4327967069 hasRelatedWork W1989560554 @default.
- W4327967069 hasRelatedWork W2008582915 @default.
- W4327967069 hasRelatedWork W2014288004 @default.
- W4327967069 hasRelatedWork W2044257351 @default.
- W4327967069 hasRelatedWork W2060255783 @default.
- W4327967069 hasRelatedWork W2065122081 @default.
- W4327967069 hasRelatedWork W2090290655 @default.
- W4327967069 hasRelatedWork W2150377117 @default.
- W4327967069 hasRelatedWork W2182839848 @default.
- W4327967069 hasRelatedWork W4230001872 @default.
- W4327967069 isParatext "false" @default.
- W4327967069 isRetracted "false" @default.
- W4327967069 workType "article" @default.