Matches in SemOpenAlex for { <https://semopenalex.org/work/W4328049325> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W4328049325 abstract "Bone mass declines with age and its maintenance is tightly linked to osteoblasts (crucial bone-building cells). Although disruption of the peripheral circadian clock is involved in various pathologies including aging-related diseases, evidence regarding how the peripheral clock regulates bone mass remains elusive. In the present study, we aimed to elucidate the effects of Bmal1 (the key activator of the peripheral circadian clock system) knockdown by lentivirus-mediated shRNA on osteoblast differentiation and its related mechanisms. We found that the expression of osteogenic markers, alkaline phosphatase activity, and mineralization were decreased, whereas apoptosis and inflammatory response were increased in Bmal1 knockdown osteoblasts. In addition, Bmal1 knockdown promoted ERK and JNK phosphorylation, as well as mTOR activity, whereas mTOR inhibition by rapamycin abrogated Bmal1 knockdown-mediated effects on osteoblast differentiation and mineralization capacity. Remarkably, Bmal1 knockdown in osteoblasts inhibited GSK3β/β-catenin signaling with decreased β-catenin expression and GSK-3β phosphorylation at serine 9, while GSK3β inhibition with TDZD-8, but not WNT3a or SKL2001, rescued Bmal1 knockdown-induced defects in osteoblast differentiation. Moreover, rapamycin partly nullified the suppression of Bmal1 knockdown on β-catenin expression and GSK-3β phosphorylation. Collectively, overall data indicated that circadian gene Bmal1 regulated osteoblast differentiation and inflammatory response in an mTOR/GSK3β/β-catenin-dependent manner, and thereby may contribute to the mineralization process and bone modeling/remodeling." @default.
- W4328049325 created "2023-03-22" @default.
- W4328049325 creator A5018067699 @default.
- W4328049325 creator A5024798204 @default.
- W4328049325 creator A5035873669 @default.
- W4328049325 creator A5044927159 @default.
- W4328049325 creator A5068009721 @default.
- W4328049325 creator A5071931173 @default.
- W4328049325 creator A5075054944 @default.
- W4328049325 date "2023-03-21" @default.
- W4328049325 modified "2023-10-18" @default.
- W4328049325 title "BMAL1 regulates osteoblast differentiation through mTOR/GSK3β/β-catenin pathway" @default.
- W4328049325 doi "https://doi.org/10.1530/jme-22-0181" @default.
- W4328049325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36942818" @default.
- W4328049325 hasPublicationYear "2023" @default.
- W4328049325 type Work @default.
- W4328049325 citedByCount "0" @default.
- W4328049325 crossrefType "journal-article" @default.
- W4328049325 hasAuthorship W4328049325A5018067699 @default.
- W4328049325 hasAuthorship W4328049325A5024798204 @default.
- W4328049325 hasAuthorship W4328049325A5035873669 @default.
- W4328049325 hasAuthorship W4328049325A5044927159 @default.
- W4328049325 hasAuthorship W4328049325A5068009721 @default.
- W4328049325 hasAuthorship W4328049325A5071931173 @default.
- W4328049325 hasAuthorship W4328049325A5075054944 @default.
- W4328049325 hasConcept C11960822 @default.
- W4328049325 hasConcept C134018914 @default.
- W4328049325 hasConcept C173396325 @default.
- W4328049325 hasConcept C185592680 @default.
- W4328049325 hasConcept C190283241 @default.
- W4328049325 hasConcept C202751555 @default.
- W4328049325 hasConcept C2778260815 @default.
- W4328049325 hasConcept C55493867 @default.
- W4328049325 hasConcept C62478195 @default.
- W4328049325 hasConcept C86554907 @default.
- W4328049325 hasConcept C86803240 @default.
- W4328049325 hasConcept C95444343 @default.
- W4328049325 hasConceptScore W4328049325C11960822 @default.
- W4328049325 hasConceptScore W4328049325C134018914 @default.
- W4328049325 hasConceptScore W4328049325C173396325 @default.
- W4328049325 hasConceptScore W4328049325C185592680 @default.
- W4328049325 hasConceptScore W4328049325C190283241 @default.
- W4328049325 hasConceptScore W4328049325C202751555 @default.
- W4328049325 hasConceptScore W4328049325C2778260815 @default.
- W4328049325 hasConceptScore W4328049325C55493867 @default.
- W4328049325 hasConceptScore W4328049325C62478195 @default.
- W4328049325 hasConceptScore W4328049325C86554907 @default.
- W4328049325 hasConceptScore W4328049325C86803240 @default.
- W4328049325 hasConceptScore W4328049325C95444343 @default.
- W4328049325 hasIssue "4" @default.
- W4328049325 hasLocation W43280493251 @default.
- W4328049325 hasLocation W43280493252 @default.
- W4328049325 hasOpenAccess W4328049325 @default.
- W4328049325 hasPrimaryLocation W43280493251 @default.
- W4328049325 hasRelatedWork W1570274999 @default.
- W4328049325 hasRelatedWork W1997721198 @default.
- W4328049325 hasRelatedWork W2045005486 @default.
- W4328049325 hasRelatedWork W2055763907 @default.
- W4328049325 hasRelatedWork W2135050229 @default.
- W4328049325 hasRelatedWork W2282386682 @default.
- W4328049325 hasRelatedWork W2990912547 @default.
- W4328049325 hasRelatedWork W2995191453 @default.
- W4328049325 hasRelatedWork W3021411624 @default.
- W4328049325 hasRelatedWork W817347 @default.
- W4328049325 hasVolume "70" @default.
- W4328049325 isParatext "false" @default.
- W4328049325 isRetracted "false" @default.
- W4328049325 workType "article" @default.