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• W4328089961 abstract "Abstract Persistent viruses include members of the family Endornavirus that cause no apparent disease and are transmitted exclusively via seed or pollen. It is speculated that these RNA viruses may be mutualists that enhance plant resilience to biotic and abiotic stresses. Using reverse transcription coupled polymerase chain reactions, we investigated if common bean ( Phaseolus vulgaris L.) varieties popular in east Africa were hosts for Phaseolus vulgaris endornavirus (PvEV) 1, 2 or 3. Out of 26 bean varieties examined, four were infected with PvEV1, three were infected with both PvEV1 and PvEV2 and three had infections of all three (PvEV) 1, 2 and 3. Notably, this was the first identification of PvEV3 in common bean from Africa. Using high throughput sequencing of two east African bean varieties (KK022 and KK072), we confirmed the presence of these viruses and generated their genomes. Intra- and inter-species sequence comparisons of these genomes with comparator sequences from GenBank revealed clear species demarcation. In addition, phylogenetic analyses based on sequences generated from the helicase domains showed that geographical distribution does not correlate to genetic relatedness or the occurrence of endornaviruses. These findings are an important first step toward future investigations to determine if these viruses engender positive effects in common bean, a vital crop in east Africa." @default.
• W4328089961 created "2023-03-22" @default.
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• W4328089961 date "2023-03-21" @default.
• W4328089961 modified "2023-09-27" @default.
• W4328089961 title "Identification and Characterisation of Phaseolus vulgaris endornavirus 1, 2 and 3 in Common Bean Cultivars of East Africa" @default.
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• W4328089961 doi "https://doi.org/10.21203/rs.3.rs-2697506/v1" @default.
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