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• W4328101222 endingPage "124089" @default.
• W4328101222 startingPage "124089" @default.
• W4328101222 abstract "Biomolecular interactions underpin most processes inside the cell. Hence, a precise and quantitative understanding of molecular association and dissociation events is crucial, not only from a fundamental perspective, but also for the rational design of biomolecular platforms for state-of-the-art biomedical and industrial applications. In this context, atomic force microscopy (AFM) appears as an invaluable experimental technique, allowing the measurement of the mechanical strength of biomolecular complexes to provide a quantitative characterization of their interaction properties from a single molecule perspective. In the present review, the most recent methodological advances in this field are presented with special focus on bioconjugation, immobilization and AFM tip functionalization, dynamic force spectroscopy measurements, molecular recognition imaging and theoretical modeling. We expect this work to significantly aid in grasping the principles of AFM-based force spectroscopy (AFM-FS) technique and provide the necessary tools to acquaint the type of data that can be achieved from this type of experiments. Furthermore, a critical assessment is done with other nanotechnology techniques to better visualize the future prospects of AFM-FS." @default.
• W4328101222 created "2023-03-22" @default.
• W4328101222 creator A5004292161 @default.
• W4328101222 creator A5013200498 @default.
• W4328101222 creator A5040689044 @default.
• W4328101222 creator A5045335216 @default.
• W4328101222 creator A5086054933 @default.
• W4328101222 date "2023-05-01" @default.
• W4328101222 modified "2023-10-16" @default.
• W4328101222 title "Recent advances in sensing the inter-biomolecular interactions at the nanoscale – A comprehensive review of AFM-based force spectroscopy" @default.
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