Matches in SemOpenAlex for { <https://semopenalex.org/work/W4328102465> ?p ?o ?g. }
- W4328102465 endingPage "237" @default.
- W4328102465 startingPage "231" @default.
- W4328102465 abstract "Antimicrobial-resistant bacteria of the order Enterobacterales are emerging threats to global public and animal health, leading to morbidity and mortality. The emergence of antimicrobial-resistant, livestock-associated pathogens is a great public health concern. The genera Enterobacter and Lelliottia are ubiquitous, facultatively anaerobic, motile, non-spore-forming, rod-shaped Gram-negative bacteria belonging to the Enterobacteriaceae family and include pathogens of public health importance. Here, we report the first draft genome sequences of a rare Lelliottia nimipressuralis strain MEZLN61 and two Enterobacter kobei strains MEZEK193 and MEZEK194 in Africa. The bacteria were isolated from environmental wastewater samples. Bacteria were cultured on nutrient agar, and the pure cultures were subjected to whole-genome sequencing. Genomic DNA was sequenced using an Illumina MiSeq platform. Generated reads were trimmed and subjected to de novo assembly. The assembled contigs were analysed for virulence genes, antimicrobial resistance genes, and extra-chromosomal plasmids, and multilocus sequence typing was performed. To compare the sequenced strains with other, previously sequenced E. kobei and L. nimipressuralis strains, available raw read sequences were downloaded, and all sequence files were treated identically to generate core genome bootstrapped maximum likelihood phylogenetic trees. Whole-genome sequencing analyses identified strain MEZLN61 as L. nimipressuralis and strains MEZEK193 and MEZEK194 as E. kobei. MEZEK193 and MEZEK194 carried genes encoding resistance to fosfomycin (fosA), beta-lactam antibiotics (blaACT-9), and colistin (mcr-9). Additionally, MEZEK193 harboured nine different virulence genes, while MEZEK194 harboured eleven different virulence genes. The phenotypic analysis showed that L. nimipressuralis strain MEZLN61 was susceptible to colistin (2 μg/mL), while E. kobei MEZEK193 (64 μg/mL) and MEZEK194 (32 μg/mL) were resistant to colistin. The genome sequences of strains L. nimipressuralis MEZLN6, E. kobei MEZEK193, and E. kobei MEZEK194 will serve as a reference point for molecular epidemiological studies of L. nimipressuralis and E. kobei in Africa. In addition, this study provides an in-depth analysis of the genomic structure and offers important information that helps clarify the pathogenesis and antimicrobial resistance of L. nimipressuralis and E. kobei. The detection of mcr-9, which is associated with very low-level colistin resistance in Enterobacter species, is alarming and may indicate the undetected dissemination of mcr genes in bacteria of the order Enterobacterales. Continuous monitoring and surveillance of the prevalence of mcr genes and their associated phenotypic changes in clinically important pathogens and environmentally associated bacteria is necessary to control and prevent the spread of colistin resistance." @default.
- W4328102465 created "2023-03-22" @default.
- W4328102465 creator A5012557095 @default.
- W4328102465 creator A5042544367 @default.
- W4328102465 creator A5079253731 @default.
- W4328102465 creator A5088256857 @default.
- W4328102465 date "2023-06-01" @default.
- W4328102465 modified "2023-10-01" @default.
- W4328102465 title "Draft genome sequences of rare Lelliottia nimipressuralis strain MEZLN61 and two Enterobacter kobei strains MEZEK193 and MEZEK194 carrying mobile colistin resistance gene mcr-9 isolated from wastewater in South Africa" @default.
- W4328102465 cites W1982865332 @default.
- W4328102465 cites W2041215841 @default.
- W4328102465 cites W2044457348 @default.
- W4328102465 cites W2101139915 @default.
- W4328102465 cites W2105486091 @default.
- W4328102465 cites W2118442768 @default.
- W4328102465 cites W2121055398 @default.
- W4328102465 cites W2122049746 @default.
- W4328102465 cites W2137494442 @default.
- W4328102465 cites W2223644615 @default.
- W4328102465 cites W2582567232 @default.
- W4328102465 cites W2809484127 @default.
- W4328102465 cites W2946204549 @default.
- W4328102465 cites W2956936996 @default.
- W4328102465 cites W2964670036 @default.
- W4328102465 cites W2968397537 @default.
- W4328102465 cites W3112457372 @default.
- W4328102465 cites W3114338642 @default.
- W4328102465 cites W3120086529 @default.
- W4328102465 cites W3156458251 @default.
- W4328102465 cites W3157990845 @default.
- W4328102465 cites W3172592458 @default.
- W4328102465 cites W3184606332 @default.
- W4328102465 cites W3194870248 @default.
- W4328102465 cites W3199883598 @default.
- W4328102465 cites W4249457105 @default.
- W4328102465 cites W4294204007 @default.
- W4328102465 doi "https://doi.org/10.1016/j.jgar.2023.03.007" @default.
- W4328102465 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36948496" @default.
- W4328102465 hasPublicationYear "2023" @default.
- W4328102465 type Work @default.
- W4328102465 citedByCount "0" @default.
- W4328102465 crossrefType "journal-article" @default.
- W4328102465 hasAuthorship W4328102465A5012557095 @default.
- W4328102465 hasAuthorship W4328102465A5042544367 @default.
- W4328102465 hasAuthorship W4328102465A5079253731 @default.
- W4328102465 hasAuthorship W4328102465A5088256857 @default.
- W4328102465 hasBestOaLocation W43281024651 @default.
- W4328102465 hasConcept C104317684 @default.
- W4328102465 hasConcept C132533263 @default.
- W4328102465 hasConcept C135763542 @default.
- W4328102465 hasConcept C141231307 @default.
- W4328102465 hasConcept C22744801 @default.
- W4328102465 hasConcept C24432333 @default.
- W4328102465 hasConcept C2776666601 @default.
- W4328102465 hasConcept C2778983983 @default.
- W4328102465 hasConcept C2779259085 @default.
- W4328102465 hasConcept C4937899 @default.
- W4328102465 hasConcept C523546767 @default.
- W4328102465 hasConcept C54355233 @default.
- W4328102465 hasConcept C547475151 @default.
- W4328102465 hasConcept C60987743 @default.
- W4328102465 hasConcept C77434933 @default.
- W4328102465 hasConcept C86803240 @default.
- W4328102465 hasConcept C89423630 @default.
- W4328102465 hasConcept C94665300 @default.
- W4328102465 hasConceptScore W4328102465C104317684 @default.
- W4328102465 hasConceptScore W4328102465C132533263 @default.
- W4328102465 hasConceptScore W4328102465C135763542 @default.
- W4328102465 hasConceptScore W4328102465C141231307 @default.
- W4328102465 hasConceptScore W4328102465C22744801 @default.
- W4328102465 hasConceptScore W4328102465C24432333 @default.
- W4328102465 hasConceptScore W4328102465C2776666601 @default.
- W4328102465 hasConceptScore W4328102465C2778983983 @default.
- W4328102465 hasConceptScore W4328102465C2779259085 @default.
- W4328102465 hasConceptScore W4328102465C4937899 @default.
- W4328102465 hasConceptScore W4328102465C523546767 @default.
- W4328102465 hasConceptScore W4328102465C54355233 @default.
- W4328102465 hasConceptScore W4328102465C547475151 @default.
- W4328102465 hasConceptScore W4328102465C60987743 @default.
- W4328102465 hasConceptScore W4328102465C77434933 @default.
- W4328102465 hasConceptScore W4328102465C86803240 @default.
- W4328102465 hasConceptScore W4328102465C89423630 @default.
- W4328102465 hasConceptScore W4328102465C94665300 @default.
- W4328102465 hasLocation W43281024651 @default.
- W4328102465 hasLocation W43281024652 @default.
- W4328102465 hasOpenAccess W4328102465 @default.
- W4328102465 hasPrimaryLocation W43281024651 @default.
- W4328102465 hasRelatedWork W1932581149 @default.
- W4328102465 hasRelatedWork W2228167218 @default.
- W4328102465 hasRelatedWork W2318106903 @default.
- W4328102465 hasRelatedWork W2413199710 @default.
- W4328102465 hasRelatedWork W2524850911 @default.
- W4328102465 hasRelatedWork W2617083140 @default.
- W4328102465 hasRelatedWork W3105924399 @default.
- W4328102465 hasRelatedWork W3162070194 @default.
- W4328102465 hasRelatedWork W4328102465 @default.
- W4328102465 hasRelatedWork W4380362593 @default.
- W4328102465 hasVolume "33" @default.