FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4328102528> ?p ?o ?g. }

• W4328102528 endingPage "1687" @default.
• W4328102528 startingPage "1675" @default.
• W4328102528 abstract "CRISPR-Cas13-mediated viral genome targeting is a novel strategy for defending against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants. Here, we generated mRNA-encoded Cas13b targeting the open reading frame 1b (ORF1b) region to effectively degrade the RNA-dependent RNA polymerase gene. Of the 12 designed CRISPR RNAs (crRNAs), those targeting the pseudoknot site upstream of ORF1b were found to be the most effective in suppressing SARS-CoV-2 propagation. Pseudoknot-targeting Cas13b reduced expression of the spike protein and attenuated viral replication by 99%. It also inhibited the replication of multiple SARS-CoV-2 variants, exhibiting broad potency. We validated the therapeutic efficacy of this system in SARS-CoV-2-infected hACE2 transgenic mice, demonstrating that crRNA treatment significantly reduced viral titers. Our findings suggest that the pseudoknot region is a strategic site for targeted genomic degradation of SARS-CoV-2. Hence, pseudoknot-targeting Cas13b could be a breakthrough therapy for overcoming infections by SARS-CoV-2 or other RNA viruses. CRISPR-Cas13-mediated viral genome targeting is a novel strategy for defending against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants. Here, we generated mRNA-encoded Cas13b targeting the open reading frame 1b (ORF1b) region to effectively degrade the RNA-dependent RNA polymerase gene. Of the 12 designed CRISPR RNAs (crRNAs), those targeting the pseudoknot site upstream of ORF1b were found to be the most effective in suppressing SARS-CoV-2 propagation. Pseudoknot-targeting Cas13b reduced expression of the spike protein and attenuated viral replication by 99%. It also inhibited the replication of multiple SARS-CoV-2 variants, exhibiting broad potency. We validated the therapeutic efficacy of this system in SARS-CoV-2-infected hACE2 transgenic mice, demonstrating that crRNA treatment significantly reduced viral titers. Our findings suggest that the pseudoknot region is a strategic site for targeted genomic degradation of SARS-CoV-2. Hence, pseudoknot-targeting Cas13b could be a breakthrough therapy for overcoming infections by SARS-CoV-2 or other RNA viruses." @default.
• W4328102528 created "2023-03-22" @default.
• W4328102528 creator A5001750008 @default.
• W4328102528 creator A5022066826 @default.
• W4328102528 creator A5027684539 @default.
• W4328102528 creator A5029171282 @default.
• W4328102528 creator A5037869563 @default.
• W4328102528 creator A5038676593 @default.
• W4328102528 creator A5042266270 @default.
• W4328102528 creator A5059383401 @default.
• W4328102528 creator A5075839571 @default.
• W4328102528 creator A5085248909 @default.
• W4328102528 creator A5089863464 @default.
• W4328102528 date "2023-06-01" @default.
• W4328102528 modified "2023-10-18" @default.
• W4328102528 title "Pseudoknot-targeting Cas13b combats SARS-CoV-2 infection by suppressing viral replication" @default.
• W4328102528 cites W2763042654 @default.
• W4328102528 cites W2765351619 @default.
• W4328102528 cites W2979487934 @default.
• W4328102528 cites W3003400025 @default.
• W4328102528 cites W3008142620 @default.
• W4328102528 cites W3013525829 @default.
• W4328102528 cites W3015608194 @default.
• W4328102528 cites W3016048690 @default.
• W4328102528 cites W3022285953 @default.
• W4328102528 cites W3029223018 @default.
• W4328102528 cites W3029682964 @default.
• W4328102528 cites W3034184621 @default.
• W4328102528 cites W3036482641 @default.
• W4328102528 cites W3081447334 @default.
• W4328102528 cites W3092403777 @default.
• W4328102528 cites W3094968263 @default.
• W4328102528 cites W3123712752 @default.
• W4328102528 cites W3128627243 @default.
• W4328102528 cites W3139106222 @default.
• W4328102528 cites W3158499862 @default.
• W4328102528 cites W3162879212 @default.
• W4328102528 cites W3168084797 @default.
• W4328102528 cites W3173547614 @default.
• W4328102528 cites W3181579162 @default.
• W4328102528 cites W3199849883 @default.
• W4328102528 cites W4200059599 @default.
• W4328102528 cites W4280605463 @default.
• W4328102528 doi "https://doi.org/10.1016/j.ymthe.2023.03.018" @default.
• W4328102528 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36945774" @default.
• W4328102528 hasPublicationYear "2023" @default.
• W4328102528 type Work @default.
• W4328102528 citedByCount "1" @default.
• W4328102528 countsByYear W43281025282023 @default.
• W4328102528 crossrefType "journal-article" @default.
• W4328102528 hasAuthorship W4328102528A5001750008 @default.
• W4328102528 hasAuthorship W4328102528A5022066826 @default.
• W4328102528 hasAuthorship W4328102528A5027684539 @default.
• W4328102528 hasAuthorship W4328102528A5029171282 @default.
• W4328102528 hasAuthorship W4328102528A5037869563 @default.
• W4328102528 hasAuthorship W4328102528A5038676593 @default.
• W4328102528 hasAuthorship W4328102528A5042266270 @default.
• W4328102528 hasAuthorship W4328102528A5059383401 @default.
• W4328102528 hasAuthorship W4328102528A5075839571 @default.
• W4328102528 hasAuthorship W4328102528A5085248909 @default.
• W4328102528 hasAuthorship W4328102528A5089863464 @default.
• W4328102528 hasBestOaLocation W43281025281 @default.
• W4328102528 hasConcept C104317684 @default.
• W4328102528 hasConcept C105130999 @default.
• W4328102528 hasConcept C132455925 @default.
• W4328102528 hasConcept C140704245 @default.
• W4328102528 hasConcept C142724271 @default.
• W4328102528 hasConcept C159047783 @default.
• W4328102528 hasConcept C167625842 @default.
• W4328102528 hasConcept C2522874641 @default.
• W4328102528 hasConcept C2777648638 @default.
• W4328102528 hasConcept C2779134260 @default.
• W4328102528 hasConcept C3008058167 @default.
• W4328102528 hasConcept C41258723 @default.
• W4328102528 hasConcept C47289529 @default.
• W4328102528 hasConcept C524204448 @default.
• W4328102528 hasConcept C54355233 @default.
• W4328102528 hasConcept C67705224 @default.
• W4328102528 hasConcept C70721500 @default.
• W4328102528 hasConcept C71924100 @default.
• W4328102528 hasConcept C84356384 @default.
• W4328102528 hasConcept C86803240 @default.
• W4328102528 hasConcept C98108389 @default.
• W4328102528 hasConceptScore W4328102528C104317684 @default.
• W4328102528 hasConceptScore W4328102528C105130999 @default.
• W4328102528 hasConceptScore W4328102528C132455925 @default.
• W4328102528 hasConceptScore W4328102528C140704245 @default.
• W4328102528 hasConceptScore W4328102528C142724271 @default.
• W4328102528 hasConceptScore W4328102528C159047783 @default.
• W4328102528 hasConceptScore W4328102528C167625842 @default.
• W4328102528 hasConceptScore W4328102528C2522874641 @default.
• W4328102528 hasConceptScore W4328102528C2777648638 @default.
• W4328102528 hasConceptScore W4328102528C2779134260 @default.
• W4328102528 hasConceptScore W4328102528C3008058167 @default.
• W4328102528 hasConceptScore W4328102528C41258723 @default.
• W4328102528 hasConceptScore W4328102528C47289529 @default.
• W4328102528 hasConceptScore W4328102528C524204448 @default.
• W4328102528 hasConceptScore W4328102528C54355233 @default.

• next